{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T08:06:45Z","timestamp":1793261205292,"version":"4.5.0"},"reference-count":44,"publisher":"American Society for Microbiology","issue":"18","license":[{"start":{"date-parts":[[2000,9,15]],"date-time":"2000-09-15T00:00:00Z","timestamp":968976000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2000,9,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            It was recently discovered that the\n            <jats:italic>aarF<\/jats:italic>\n            gene in\n            <jats:italic>Providencia stuartii<\/jats:italic>\n            is required for coenzyme Q (CoQ) biosynthesis. Here we report that\n            <jats:italic>yigR<\/jats:italic>\n            , the\n            <jats:italic>Escherichia coli<\/jats:italic>\n            homologue of\n            <jats:italic>aarF<\/jats:italic>\n            , is\n            <jats:italic>ubiB<\/jats:italic>\n            , a gene required for the first monooxygenase step in CoQ biosynthesis. Both the\n            <jats:italic>P. stuartii aarF<\/jats:italic>\n            and\n            <jats:italic>E. coli ubiB<\/jats:italic>\n            (\n            <jats:italic>yigR<\/jats:italic>\n            ) disruption mutant strains lack CoQ and accumulate octaprenylphenol. Octaprenylphenol is the CoQ biosynthetic intermediate found to accumulate in the\n            <jats:italic>E. coli<\/jats:italic>\n            strain AN59, which contains the\n            <jats:italic>ubiB409<\/jats:italic>\n            mutant allele. Analysis of the mutation in the\n            <jats:italic>E. coli<\/jats:italic>\n            strain AN59 reveals no mutations within the\n            <jats:italic>ubiB<\/jats:italic>\n            gene, but instead shows the presence of an IS\n            <jats:italic>1<\/jats:italic>\n            element at position +516 of the\n            <jats:italic>ubiE<\/jats:italic>\n            gene. The\n            <jats:italic>ubiE<\/jats:italic>\n            gene encodes a\n            <jats:italic>C<\/jats:italic>\n            -methyltransferase required for the synthesis of both CoQ and menaquinone, and it is the 5\u2032 gene in an operon containing\n            <jats:italic>ubiE<\/jats:italic>\n            ,\n            <jats:italic>yigP<\/jats:italic>\n            , and\n            <jats:italic>ubiB<\/jats:italic>\n            . The data indicate that octaprenylphenol accumulates in AN59 as a result of a polar effect of the\n            <jats:italic>ubiE<\/jats:italic>\n            ::IS\n            <jats:italic>1<\/jats:italic>\n            mutation on the downstream\n            <jats:italic>ubiB<\/jats:italic>\n            gene. AN59 is complemented by a DNA segment containing the contiguous\n            <jats:italic>ubiE<\/jats:italic>\n            ,\n            <jats:italic>yigP<\/jats:italic>\n            , and\n            <jats:italic>ubiB<\/jats:italic>\n            genes. Although transformation of AN59 with a DNA segment containing the\n            <jats:italic>ubiB<\/jats:italic>\n            coding region fails to restore CoQ biosynthesis, transformation with the\n            <jats:italic>ubiE<\/jats:italic>\n            coding region results in a low-frequency but significant rescue attributed to homologous recombination. In addition, the\n            <jats:italic>fre<\/jats:italic>\n            gene, previously considered to correspond to\n            <jats:italic>ubiB<\/jats:italic>\n            , was found not to be involved in CoQ biosynthesis. The\n            <jats:italic>ubiB<\/jats:italic>\n            gene is a member of a predicted protein kinase family of which the\n            <jats:italic>Saccharomyces cerevisiae ABC1<\/jats:italic>\n            gene is the prototypic member. The possible protein kinase function of UbiB and Abc1 and the role these polypeptides may play in CoQ biosynthesis are discussed.\n          <\/jats:p>","DOI":"10.1128\/jb.182.18.5139-5146.2000","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T10:03:59Z","timestamp":1027764239000},"page":"5139-5146","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":133,"title":["Identification of\n            <i>Escherichia coli ubiB<\/i>\n            , a Gene Required for the First Monooxygenase Step in Ubiquinone Biosynthesis"],"prefix":"10.1128","volume":"182","author":[{"given":"Wayne W.","family":"Poon","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Department of Chemistry and Biochemistry and the Molecular Biology Institute, University of California, Los Angeles, California 90095,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diana E.","family":"Davis","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Chemistry and Biochemistry and the Molecular Biology Institute, University of California, Los Angeles, California 90095,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huan T.","family":"Ha","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Chemistry and Biochemistry and the Molecular Biology Institute, University of California, Los Angeles, California 90095,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tanya","family":"Jonassen","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Chemistry and Biochemistry and the Molecular Biology Institute, University of California, Los Angeles, California 90095,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip N.","family":"Rather","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Departments of Medicine and Molecular Biology and Microbiology, Case Western Reserve University School of Medicine, Cleveland, Ohio 441062"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine F.","family":"Clarke","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Chemistry and Biochemistry and the Molecular Biology Institute, University of California, Los Angeles, California 90095,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"4750","DOI":"10.1021\/bi00615a024","article-title":"Alternative hydroxylases for the aerobic and anaerobic biosynthesis of ubiquinone in Escherichia coli","volume":"17","author":"Alexander K.","year":"1978","unstructured":"Alexander K. 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