{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T12:37:59Z","timestamp":1790426279681,"version":"4.1.0"},"reference-count":28,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2000,2,1]],"date-time":"2000-02-01T00:00:00Z","timestamp":949363200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2000,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The enterobacterial\n                    <jats:italic>flhDC<\/jats:italic>\n                    master operon activates expression of the flagellar biogenesis gene hierarchy and also represses cell division. During\n                    <jats:italic>Proteus mirabilis<\/jats:italic>\n                    differentiation into elongated hyperflagellated swarm cells,\n                    <jats:italic>flhDC<\/jats:italic>\n                    transcription is strongly but transiently increased. We show that concentration of the FlhD and FlhC proteins is also tightly controlled at the posttranslational level. This is achieved by protein degradation, which is most severe after differentiation when the half-life of both proteins is ca. 2 min. Degradation is energy dependent and putatively involves the Lon protease.\n                  <\/jats:p>","DOI":"10.1128\/jb.182.3.833-836.2000","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T05:58:18Z","timestamp":1027749498000},"page":"833-836","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":63,"title":["Rapid Turnover of FlhD and FlhC, the Flagellar Regulon Transcriptional Activator Proteins, during\n                    <i>Proteus<\/i>\n                    Swarming"],"prefix":"10.1128","volume":"182","author":[{"given":"Laurent","family":"Claret","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Department of Pathology, University of Cambridge, Cambridge CB2 1QP, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Colin","family":"Hughes","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Pathology, University of Cambridge, Cambridge CB2 1QP, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1016\/0022-2836(76)90119-4","article-title":"Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage \u03bb and Mu","volume":"104","author":"Casadaban M. J.","year":"1976","unstructured":"Casadaban M. J. Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage \u03bb and Mu.J. Mol. Biol.1041976541555","journal-title":"J. Mol. Biol."},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.172.12.7098-7103.1990"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1998.00967.x"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.178.2.554-559.1996"},{"key":"e_1_3_2_6_2","doi-asserted-by":"crossref","first-page":"630","DOI":"10.1016\/S1369-5274(99)00033-8","article-title":"Swarming motility","volume":"2","author":"Fraser G. M.","year":"1999","unstructured":"Fraser G. M. Hughes C. Swarming motility.Curr Opin. Microbiol.21999630635","journal-title":"Curr Opin. Microbiol."},{"key":"e_1_3_2_7_2","unstructured":"Furness\nR. B.\nPh.D. thesis.\n1999\nUniversity of Cambridge\nCambridge United Kingdom"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.179.17.5585-5588.1997"},{"key":"e_1_3_2_9_2","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1146\/annurev.genet.30.1.465","article-title":"Proteases and their targets in Escherichia coli","volume":"30","author":"Gottesman S.","year":"1996","unstructured":"Gottesman S. Proteases and their targets in Escherichia coli.Annu. Rev. Genet.301996465506","journal-title":"Annu. Rev. Genet."},{"key":"e_1_3_2_10_2","doi-asserted-by":"crossref","first-page":"22618","DOI":"10.1016\/S0021-9258(18)41573-6","article-title":"ClpX, an alternative subunit for the ATP-dependent Clp protease of Escherichia coli","volume":"268","author":"Gottesman S.","year":"1993","unstructured":"Gottesman S. Clark W. P. de Crecy-Lagard V. Maurizi M. R. ClpX, an alternative subunit for the ATP-dependent Clp protease of Escherichia coli.J. Biol. Chem.26819932261822626","journal-title":"J. Biol. Chem."},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/mr.56.4.592-621.1992"},{"key":"e_1_3_2_12_2","doi-asserted-by":"crossref","first-page":"4121","DOI":"10.1128\/jb.177.14.4121-4130.1995","article-title":"Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter","volume":"177","author":"Guzman L.-M.","year":"1996","unstructured":"Guzman L.-M. Belin D. Carson M. J. Beckwith J. Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter.J. Bacteriol.177199641214130","journal-title":"J. Bacteriol."},{"key":"e_1_3_2_13_2","doi-asserted-by":"crossref","first-page":"761","DOI":"10.1111\/j.1365-2958.1995.tb02383.x","article-title":"Requirement for FlhA in flagella assembly and swarm cell differentiation by Proteus mirabilis","volume":"15","author":"Gygi D.","year":"1995","unstructured":"Gygi D. Bailey M. J. Allison C. Hughes Requirement for FlhA in flagella assembly and swarm cell differentiation by Proteus mirabilis.Mol. Microbiol.151995761769","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.179.15.4741-4746.1997"},{"key":"e_1_3_2_15_2","doi-asserted-by":"crossref","first-page":"10861","DOI":"10.1073\/pnas.90.22.10861","article-title":"Cell growth and \u03bb phage development controlled by the same essential Escherichia coli gene, ftsH\/HflB","volume":"90","author":"Herman C. T.","year":"1993","unstructured":"Herman C. T. Ogura T. Tomoyasu T. Hiraga S. Akiyama Y. Ito K. Thomas R. D'ari R. Bouloc P. Cell growth and \u03bb phage development controlled by the same essential Escherichia coli gene, ftsH\/HflB.Proc. Natl. Acad. Sci. USA9019931086110865","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"e_1_3_2_16_2","doi-asserted-by":"crossref","first-page":"3516","DOI":"10.1073\/pnas.92.8.3516","article-title":"Degradation of \u03c232, the heat shock regulator in Escherichia coli, is governed by HflB","volume":"92","author":"Herman C. T.","year":"1995","unstructured":"Herman C. T. Thevenet D. D'Ari R. Bouloc P. Degradation of \u03c232, the heat shock regulator in Escherichia coli, is governed by HflB.Proc. Natl. Acad. Sci. USA92199535163520","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"e_1_3_2_17_2","doi-asserted-by":"crossref","first-page":"5658","DOI":"10.1093\/emboj\/17.19.5658","article-title":"An essential protease involved in bacterial cell-cycle control","volume":"17","author":"Jenal U.","year":"1998","unstructured":"Jenal U. Fuchs T. An essential protease involved in bacterial cell-cycle control.EMBO J.17199856585669","journal-title":"EMBO J."},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.169.4.1509-1515.1987"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.180.6.1573-1577.1998"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.176.23.7345-7351.1994"},{"key":"e_1_3_2_21_2","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1146\/annurev.ge.26.120192.001023","article-title":"Genetics and biogenesis of bacterial flagella","volume":"26","author":"Macnab R. M.","year":"1992","unstructured":"Macnab R. M. Genetics and biogenesis of bacterial flagella.Annu. Rev. Genet.261992131158","journal-title":"Annu. Rev. Genet."},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.164.3.1124-1135.1985"},{"key":"e_1_3_2_23_2","doi-asserted-by":"crossref","first-page":"8227","DOI":"10.1073\/pnas.87.21.8227","article-title":"Azide resistant mutants of Escherichia coli alter the SecA protein, an azide-sensitive component of the protein export machinery","volume":"87","author":"Oliver D. B.","year":"1990","unstructured":"Oliver D. B. Cabelli R. J. Dolan K. M. Jarosik G. P. Azide resistant mutants of Escherichia coli alter the SecA protein, an azide-sensitive component of the protein export machinery.Proc. Natl. Acad. Sci. USA87199082278231","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.178.3.668-674.1996"},{"key":"e_1_3_2_25_2","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/S0092-8674(00)80995-2","article-title":"Cell cycle control of an essential bacterial two-component signal transduction protein","volume":"84","author":"Quon K. C.","year":"1996","unstructured":"Quon K. C. Marczynski G. T. Shapiro L. Cell cycle control of an essential bacterial two-component signal transduction protein.Cell8419968393","journal-title":"Cell"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.173.14.4254-4262.1991"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.179.1.107-114.1997"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(86)90385-2"},{"key":"e_1_3_2_29_2","doi-asserted-by":"crossref","first-page":"1532","DOI":"10.1101\/gad.10.12.1532","article-title":"Caulobacter Lon protease has a critical role in cell-cycle control of DNA methylation","volume":"10","author":"Wright R.","year":"1996","unstructured":"Wright R. Stephens C. Zweiger G. Shapiro L. Alley M. R. Caulobacter Lon protease has a critical role in cell-cycle control of DNA methylation.Genes Dev.10199615321542","journal-title":"Genes Dev."}],"container-title":["Journal of Bacteriology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JB.182.3.833-836.2000","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JB.182.3.833-836.2000","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T13:56:10Z","timestamp":1627566970000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JB.182.3.833-836.2000"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,2]]},"references-count":28,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2000,2]]}},"alternative-id":["10.1128\/JB.182.3.833-836.2000"],"URL":"https:\/\/doi.org\/10.1128\/jb.182.3.833-836.2000","relation":{},"ISSN":["0021-9193","1098-5530"],"issn-type":[{"value":"0021-9193","type":"print"},{"value":"1098-5530","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,2]]},"assertion":[{"value":"1999-09-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"1999-11-11","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-20","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}