{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T16:19:11Z","timestamp":1790698751261,"version":"4.1.0"},"reference-count":24,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2000,3,1]],"date-time":"2000-03-01T00:00:00Z","timestamp":951868800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2000,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Growth of a wall-less, L-form of\n            <jats:italic>Escherichia coli<\/jats:italic>\n            specifically requires calcium, and in its absence, cells ceased dividing, became spherical, swelled, developed large vacuoles, and eventually lysed. The key cell division protein, FtsZ, was present in the L-form at a concentration five times less than that in the parental strain. One interpretation of these results is that the L-form possesses an enzoskeleton partly regulated by calcium.\n          <\/jats:p>","DOI":"10.1128\/jb.182.5.1419-1422.2000","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T10:01:09Z","timestamp":1027764069000},"page":"1419-1422","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":62,"title":["Effects of Calcium and Calcium Chelators on Growth and Morphology of\n            <i>Escherichia coli<\/i>\n            L-Form NC-7"],"prefix":"10.1128","volume":"182","author":[{"given":"T.","family":"Onoda","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Department of Biological Science, Faculty of Life and Environmental Science, Shimane University, Matsue 690, Japan1;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Enokizono","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Biological Science, Faculty of Life and Environmental Science, Shimane University, Matsue 690, Japan1;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Kaya","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Biological Science, Faculty of Life and Environmental Science, Shimane University, Matsue 690, Japan1;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Oshima","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Biological Science, Faculty of Life and Environmental Science, Shimane University, Matsue 690, Japan1;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Freestone","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Department of Microbiology and Immunology, University of Leicester, Leicester LE1 9HN, United Kingdom2; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.","family":"Norris","sequence":"additional","affiliation":[{"name":"<!--label omitted: 3-->IFR \u2018Syste\u0300mes Inte\u0301gre\u0301s\u2019, Laboratoire de Microbiologie, Faculte des Sciences et Techniques de Rouen, F76821 Mont Saint Aignan Cedex, France3"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"1173","DOI":"10.1046\/j.1365-2958.1999.01426.x","article-title":"Transcription- and translation-dependent changes in membrane dynamics in bacteria: testing the transertion model for domain formation","volume":"32","author":"Binenbaum Z.","year":"1999","unstructured":"Binenbaum Z. Parola A. H. Zaritsky A. Fishov I. Transcription- and translation-dependent changes in membrane dynamics in bacteria: testing the transertion model for domain formation.Mol. Microbiol.32199911731182","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.180.23.6107-6116.1998"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.127.3.1564-1567.1976"},{"key":"e_1_3_2_5_2","doi-asserted-by":"crossref","first-page":"3289","DOI":"10.1099\/00221287-144-12-3289","article-title":"Protein phosphorylation in Escherichia coli L-form NC-7","volume":"144","author":"Freestone P.","year":"1998","unstructured":"Freestone P. Grant S. Trinei M. Onoda T. Norris V. Protein phosphorylation in Escherichia coli L-form NC-7.Microbiology144199832893295","journal-title":"Microbiology"},{"key":"e_1_3_2_6_2","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1111\/j.1365-2818.1986.tb02713.x","article-title":"A method for observing intracellular structures of free cells by scanning electron microscopy","volume":"141","author":"Fukudome H.","year":"1986","unstructured":"Fukudome H. Tanaka K. A method for observing intracellular structures of free cells by scanning electron microscopy.J. Micros.1411986171178","journal-title":"J. Micros."},{"key":"e_1_3_2_7_2","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1002\/jobm.3630231004","article-title":"Ultrastructural characterization of core structures and paracrystalline inclusion bodies in L-form cells of streptomycetes","volume":"23","author":"Gumpert J.","year":"1983","unstructured":"Gumpert J. Ultrastructural characterization of core structures and paracrystalline inclusion bodies in L-form cells of streptomycetes.Z. Allg. Mikrobiol.231983625633","journal-title":"Z. Allg. Mikrobiol."},{"key":"e_1_3_2_8_2","doi-asserted-by":"crossref","unstructured":"Gumpert\nJ.\nCellular growth without a murein sacculus\u2014the nucleoid-associated compartmentation concept\nBacterial growth and lysis. Metabolism and structure of the bacterial sacculus.\nde Pedro\nM. A.\nHoltje\nJ.-V.\nLoffelhardt\nW.\n1992\n453\n463\nPlenum Press\nNew York N.Y","DOI":"10.1007\/978-1-4757-9359-8_52"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.75.2.143-160.1958"},{"key":"e_1_3_2_10_2","doi-asserted-by":"crossref","first-page":"915","DOI":"10.1016\/S0300-9084(99)00203-5","article-title":"Hypothesis: hyperstructures regulate bacterial structure and the cell cycle","volume":"81","author":"Norris V.","year":"1999","unstructured":"Norris V. Alexandre S. Bouligand Y. Cellier D. Demarty M. Grehan G. Gouesbet G. Guespin J. Insinna E. Le Sceller L. Maheu B. Monnier C. Grant N. Onoda T. Orange N. Oshima A. Picton L. Polaert H. Ripoll C. Thellier M. Valleton J.-M. Verdus M.-C. Vincent J.-C. White G. Wiggins P. Hypothesis: hyperstructures regulate bacterial structure and the cell cycle.Biochimie811999915920","journal-title":"Biochimie"},{"key":"e_1_3_2_11_2","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1111\/j.1365-2958.1991.tb00748.x","article-title":"Calcium in bacteria: a solution to which problem?","volume":"5","author":"Norris V.","year":"1991","unstructured":"Norris V. Chen M. Goldberg M. Voskuil J. McGurk M. Holland I. B. Calcium in bacteria: a solution to which problem? Mol. Microbiol. 5 1991 775 778","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_12_2","doi-asserted-by":"crossref","first-page":"1592","DOI":"10.1046\/j.1365-2958.1999.01275.x","article-title":"Metabolite-induced metabolons: the activation of transporter-enzyme complexes by substrate binding","volume":"31","author":"Norris V.","year":"1999","unstructured":"Norris V. Gascuel P. Guespin-Michel J. Ripoll C. Saier M. H. Jr. Metabolite-induced metabolons: the activation of transporter-enzyme complexes by substrate binding.Mol. Microbiol.31199915921595","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.178.13.3677-3682.1996"},{"key":"e_1_3_2_14_2","first-page":"71","article-title":"The mechanical origins of life","volume":"49","author":"Norris V.","year":"1999","unstructured":"Norris V. Onoda T. Pollaert H. Grehan G. The mechanical origins of life.Biosystems4919997178","journal-title":"Biosystems"},{"key":"e_1_3_2_15_2","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1046\/j.1365-2958.1996.373899.x","article-title":"The Escherichia coli enzoskeleton","volume":"19","author":"Norris V.","year":"1996","unstructured":"Norris V. Turnock G. Sigee D. The Escherichia coli enzoskeleton.Mol. Microbiol.191996197204","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_16_2","first-page":"3071","article-title":"Effects of Ca2+ and a protonophore on growth of an Escherichia coli L-form","volume":"134","author":"Onoda T.","year":"1988","unstructured":"Onoda T. Oshima A. Effects of Ca2+ and a protonophore on growth of an Escherichia coli L-form.J. Gen. Microbiol.134198830713077","journal-title":"J. Gen. Microbiol."},{"key":"e_1_3_2_17_2","first-page":"527","article-title":"Morphology, growth and reversion in a stable L-form of Escherichia coli K12","volume":"133","author":"Onoda T.","year":"1987","unstructured":"Onoda T. Oshima A. Nakano S. Matsuno A. Morphology, growth and reversion in a stable L-form of Escherichia coli K12.J. Gen. Microbiol.1331987527534","journal-title":"J. Gen. Microbiol."},{"key":"e_1_3_2_18_2","first-page":"69","article-title":"Cation\/proton antiport systems in Escherichia coli K12, L-form NC-7","volume":"20","author":"Onoda T.","year":"1989","unstructured":"Onoda T. Shinjou H. Oshima A. Cation\/proton antiport systems in Escherichia coli K12, L-form NC-7.Mem. Fac. Sci. Shimane Univ.2019896976","journal-title":"Mem. Fac. Sci. Shimane Univ."},{"key":"e_1_3_2_19_2","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1111\/j.1365-2672.1987.tb05161.x","article-title":"L-forms: evolution or revolution?","volume":"63","author":"Paton A. M.","year":"1987","unstructured":"Paton A. M. L-forms: evolution or revolution? J. App. Bacteriol. 63 1987 365 371","journal-title":"J. App. Bacteriol."},{"key":"e_1_3_2_20_2","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/S0065-2911(08)60144-7","article-title":"Calcium and bacteria","volume":"37","author":"Smith R. J.","year":"1995","unstructured":"Smith R. J. Calcium and bacteria.Adv. Microb. Physiol.37199583103","journal-title":"Adv. Microb. Physiol."},{"key":"e_1_3_2_21_2","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1111\/j.1574-6968.1995.tb07462.x","article-title":"Identification of novel phosphoproteins in Escherichia coli using the gene-protein database","volume":"127","author":"Sweeney S. T.","year":"1995","unstructured":"Sweeney S. T. Freestone P. Norris V. Identification of novel phosphoproteins in Escherichia coli using the gene-protein database.FEMS Microbiol. Lett.1271995133138","journal-title":"FEMS Microbiol. Lett."},{"key":"e_1_3_2_22_2","first-page":"66","article-title":"OsO4-tannin-OsO4 fixation and staining of biological specimens for electron microscopy","volume":"27","author":"Takahashi G.","year":"1978","unstructured":"Takahashi G. OsO4-tannin-OsO4 fixation and staining of biological specimens for electron microscopy.J. Electron Microsc.27197866","journal-title":"J. Electron Microsc."},{"key":"e_1_3_2_23_2","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1111\/j.1472-765X.1996.tb01157.x","article-title":"CCD detection of lux-marked Pseudomonas syringae pv. phaseolicola L-forms associated with Chinese cabbage and the resulting disease protection against Xanthomonas campestris","volume":"22","author":"Waterhouse R. N.","year":"1996","unstructured":"Waterhouse R. N. Buhariwalla H. Bourn D. Rattray E. J. Glover L. A. CCD detection of lux-marked Pseudomonas syringae pv. phaseolicola L-forms associated with Chinese cabbage and the resulting disease protection against Xanthomonas campestris.Lett. App. Microbiol.221996262266","journal-title":"Lett. App. Microbiol."},{"key":"e_1_3_2_24_2","first-page":"171","article-title":"The toxicity of metal chelate complexes of EGTA precludes the use of EGTA buffered media for the fungi Allomyces and Achlya","volume":"79","author":"Youatt J.","year":"1994","unstructured":"Youatt J. The toxicity of metal chelate complexes of EGTA precludes the use of EGTA buffered media for the fungi Allomyces and Achlya.Microbios791994171185","journal-title":"Microbios"},{"key":"e_1_3_2_25_2","doi-asserted-by":"crossref","first-page":"5455","DOI":"10.1093\/emboj\/16.17.5455","article-title":"Ca2+-mediated GTP-dependent assembly of bacterial cell division protein FtsZ into asters and polymer networks in vitro","volume":"16","author":"Yu X.-C.","year":"1997","unstructured":"Yu X.-C. Margolin W. Ca2+-mediated GTP-dependent assembly of bacterial cell division protein FtsZ into asters and polymer networks in vitro.EMBO J.16199754555463","journal-title":"EMBO J."}],"container-title":["Journal of Bacteriology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JB.182.5.1419-1422.2000","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JB.182.5.1419-1422.2000","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T17:56:37Z","timestamp":1627581397000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JB.182.5.1419-1422.2000"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,3]]},"references-count":24,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2000,3]]}},"alternative-id":["10.1128\/JB.182.5.1419-1422.2000"],"URL":"https:\/\/doi.org\/10.1128\/jb.182.5.1419-1422.2000","relation":{},"ISSN":["0021-9193","1098-5530"],"issn-type":[{"value":"0021-9193","type":"print"},{"value":"1098-5530","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,3]]},"assertion":[{"value":"1999-07-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"1999-11-18","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-20","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}