{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T20:33:05Z","timestamp":1790973185581,"version":"4.1.0"},"reference-count":40,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2000,4,1]],"date-time":"2000-04-01T00:00:00Z","timestamp":954547200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2000,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The biphenyl and salicylate metabolic pathways in\n            <jats:italic>Pseudomonas putida<\/jats:italic>\n            KF715 are chromosomally encoded. The\n            <jats:italic>bph<\/jats:italic>\n            gene cluster coding for the conversion of biphenyl to benzoic acid and the\n            <jats:italic>sal<\/jats:italic>\n            gene cluster coding for the salicylate\n            <jats:italic>meta<\/jats:italic>\n            -pathway were obtained from the KF715 genomic cosmid libraries. These two gene clusters were separated by 10-kb DNA and were highly prone to deletion when KF715 was grown in nutrient medium. Two types of deletions took place at the region including only the\n            <jats:italic>bph<\/jats:italic>\n            genes (ca. 40 kb) or at the region including both the\n            <jats:italic>bph<\/jats:italic>\n            and\n            <jats:italic>sal<\/jats:italic>\n            genes (ca. 70 kb). A 90-kb DNA region, including both the\n            <jats:italic>bph<\/jats:italic>\n            and\n            <jats:italic>sal<\/jats:italic>\n            genes (termed the\n            <jats:italic>bph-sal<\/jats:italic>\n            element), was transferred by conjugation from KF715 to\n            <jats:italic>P. putida<\/jats:italic>\n            AC30. Such transconjugants gained the ability to grow on biphenyl and salicylate as the sole sources of carbon. The\n            <jats:italic>bph<\/jats:italic>\n            and\n            <jats:italic>sal<\/jats:italic>\n            element was located on the chromosome of the recipient. The\n            <jats:italic>bph-sal<\/jats:italic>\n            element in strain AC30 was also highly prone to deletion; however, it could be mobilized to the chromosome of\n            <jats:italic>P. putida<\/jats:italic>\n            KT2440 and the two deletion mutants of KF715.\n          <\/jats:p>","DOI":"10.1128\/jb.182.7.1949-1955.2000","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T10:01:09Z","timestamp":1027764069000},"page":"1949-1955","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":64,"title":["A 90-Kilobase Conjugative Chromosomal Element Coding for Biphenyl and Salicylate Catabolism in\n            <i>Pseudomonas putida<\/i>\n            KF715"],"prefix":"10.1128","volume":"182","author":[{"given":"Akito","family":"Nishi","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Division of Bioresource and Bioenvironmental Sciences, Graduate School, Kyushu University, Hakozaki, Fukuoka 812-8581, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kiyomi","family":"Tominaga","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Division of Bioresource and Bioenvironmental Sciences, Graduate School, Kyushu University, Hakozaki, Fukuoka 812-8581, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kensuke","family":"Furukawa","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Division of Bioresource and Bioenvironmental Sciences, Graduate School, Kyushu University, Hakozaki, Fukuoka 812-8581, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"241","DOI":"10.3109\/07388559009038210","article-title":"Aerobic and anaerobic biodegradation of PCBs: a review","volume":"10","author":"Abramowicz D. 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