{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T16:31:09Z","timestamp":1760977869865},"reference-count":48,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2000,4,15]],"date-time":"2000-04-15T00:00:00Z","timestamp":955756800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2000,4,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Haemophilus ducreyi<\/jats:italic>\n            is the etiologic agent of chancroid, a genital ulcer disease. The lipooligosaccharide (LOS) is considered to be a major virulence determinant and has been implicated in the adherence of\n            <jats:italic>H. ducreyi<\/jats:italic>\n            to keratinocytes. Strain A77, an isolate from the Paris collection, is serum sensitive, poorly adherent to fibroblasts, and deficient in microcolony formation. Structural analysis indicates that the LOS of strain A77 lacks the galactose residue found in the\n            <jats:italic>N<\/jats:italic>\n            -acetyllactosamine portion of the strain 35000HP LOS as well as the sialic acid substitution. From an\n            <jats:italic>H. ducreyi<\/jats:italic>\n            35000HP genomic DNA library, a clone complementing the defect in A77 was identified by immunologic screening with monoclonal antibody (MAb) 3F11, a MAb which recognizes the\n            <jats:italic>N<\/jats:italic>\n            -acetyllactosamine portion of strain 35000HP LOS. The clone contained a 4-kb insert that was sequenced. One open reading frame which encodes a protein with a molecular weight of 33,400 was identified. This protein has homology to glycosyltransferases of\n            <jats:italic>Haemophilus influenzae<\/jats:italic>\n            ,\n            <jats:italic>Haemophilus somnus<\/jats:italic>\n            ,\n            <jats:italic>Neisseria<\/jats:italic>\n            species, and\n            <jats:italic>Pasteurella haemolytica<\/jats:italic>\n            . The putative\n            <jats:italic>H. ducreyi<\/jats:italic>\n            glycosyltransferase gene was insertionally inactivated, and an isogenic mutant of strain 35000HP was constructed. The most complex LOS glycoform produced by the mutant has a mobility on sodium dodecyl sulfate-polyacrylamide gel identical to that of the LOS of strain A77 and lacks the 3F11-binding epitope. Structural studies confirm that the most complex glycoform of the LOS isolated from the mutant lacks the galactose residue found in the\n            <jats:italic>N<\/jats:italic>\n            -acetyllactosamine portion of the strain 35000HP LOS. Although previously published data suggested that the serum-sensitive phenotype of A77 was due to the LOS mutation, we observed that the complemented A77 strain retained its serum-sensitive phenotype and that the galactosyltransferase mutant retained its serum-resistant phenotype. Thus, the serum sensitivity of strain A77 cannot be attributed to the galactosyltransferase mutation in strain A77.\n          <\/jats:p>","DOI":"10.1128\/jb.182.8.2292-2298.2000","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T10:01:09Z","timestamp":1027764069000},"page":"2292-2298","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":22,"title":["Cloning and Characterization of the Lipooligosaccharide Galactosyltransferase II Gene of\n            <i>Haemophilus ducreyi<\/i>"],"prefix":"10.1128","volume":"182","author":[{"given":"Shuhua","family":"Sun","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Children's Research Institute,1"},{"name":"<!--label omitted: 2-->Department of Molecular Virology, Immunology and Medical Genetics,2The Ohio State University, Columbus, Ohio 43205-2696, and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Birgit","family":"Schilling","sequence":"additional","affiliation":[{"name":"<!--label omitted: 3-->Department of Pharmaceutical Chemistry, University of California, San Francisco, California 94143-04463"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laurie","family":"Tarantino","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Children's Research Institute,1"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael V.","family":"Tullius","sequence":"additional","affiliation":[{"name":"<!--label omitted: 3-->Department of Pharmaceutical Chemistry, University of California, San Francisco, California 94143-04463"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bradford W.","family":"Gibson","sequence":"additional","affiliation":[{"name":"<!--label omitted: 3-->Department of Pharmaceutical Chemistry, University of California, San Francisco, California 94143-04463"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr.","given":"Robert S.","family":"Munson","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Children's Research Institute,1"},{"name":"<!--label omitted: 4-->Department of Pediatrics,4 and"},{"name":"<!--label omitted: 2-->Department of Molecular Virology, Immunology and Medical Genetics,2The Ohio State University, Columbus, Ohio 43205-2696, and"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1006\/mpat.1996.0089","article-title":"Attachment of Haemophilus ducreyi to human foreskin fibroblasts involves LOS and fibronectin","volume":"22","author":"Alfa M. 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