{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T20:27:46Z","timestamp":1791232066562,"version":"4.1.0"},"reference-count":34,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2001,1,1]],"date-time":"2001-01-01T00:00:00Z","timestamp":978307200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2001,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Previous studies have demonstrated that a proportion of\n            <jats:italic>Staphylococcus aureus<\/jats:italic>\n            isolates from bovine mastitis coproduce toxic shock syndrome toxin (TSST) and staphylococcal enterotoxin C (SEC). In this study, molecular genetic analysis of one such strain, RF122, revealed the presence of a 15,891-bp putative pathogenicity island (SaPIbov) encoding the genes for TSST (\n            <jats:italic>tst<\/jats:italic>\n            ), the SEC bovine variant (\n            <jats:italic>sec<\/jats:italic>\n            -bovine), and a gene (\n            <jats:italic>sel<\/jats:italic>\n            ) which encodes an enterotoxin-like protein. The island contains 21 open reading frames specifying hypothetical proteins longer than 60 amino acids including an integrase-like gene. The element is bordered by 74-bp direct repeats at the left and right junctions, and the integration site lies adjacent to the 3\u2032 end of the GMP synthase gene (\n            <jats:italic>gmps<\/jats:italic>\n            ) in the\n            <jats:italic>S. aureus<\/jats:italic>\n            chromosome. SaPIbov contains a central region of sequence identity with the previously characterized\n            <jats:italic>tst<\/jats:italic>\n            pathogenicity island SaPI1 (J. A. Lindsay et al., Mol. Microbiol. 29:527\u2013543, 1998). A closely related strain, RF120, of the same multilocus enzyme electrophoretic type, random amplified polymorphic DNA type, and ribotype, does not contain the island, implying that the element is mobile and that a recent insertion\/deletion event has taken place. TSST and TSST\/SEC-deficient mutants of\n            <jats:italic>S. aureus<\/jats:italic>\n            strain RF122 were constructed by allele replacement. In vitro bovine V\u03b2-specific lymphocyte expansion analysis by culture supernatants of wild-type strains and of\n            <jats:italic>tst<\/jats:italic>\n            and\n            <jats:italic>sec<\/jats:italic>\n            -bovine allele replacement mutants revealed that TSST stimulates BTB13-specific T cells whereas SEC-bovine stimulates BTB93-specific T cells. This suggests that the presence of SaPIbov may contribute to modulation of the bovine immune response.\n          <\/jats:p>","DOI":"10.1128\/jb.183.1.63-70.2001","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T10:01:09Z","timestamp":1027764069000},"page":"63-70","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":252,"title":["Characterization of a Putative Pathogenicity Island from Bovine\n            <i>Staphylococcus aureus<\/i>\n            Encoding Multiple Superantigens"],"prefix":"10.1128","volume":"183","author":[{"given":"J. Ross","family":"Fitzgerald","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Department of Microbiology, Moyne Institute of Preventive Medicine,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven R.","family":"Monday","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Department of Microbiology, Molecular Biology, and Biochemistry, University of Idaho, Moscow, Idaho 838442"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy J.","family":"Foster","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Microbiology, Moyne Institute of Preventive Medicine,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregory A.","family":"Bohach","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Department of Microbiology, Molecular Biology, and Biochemistry, University of Idaho, Moscow, Idaho 838442"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick J.","family":"Hartigan","sequence":"additional","affiliation":[{"name":"<!--label omitted: 3-->Department of Physiology,3 Trinity College, Dublin 2, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William J.","family":"Meaney","sequence":"additional","affiliation":[{"name":"<!--label omitted: 4-->Dairy Production Research Centre, Teagasc, Moorepark, Fermoy, County Cork,4 Republic of Ireland, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cyril J.","family":"Smyth","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Microbiology, Moyne Institute of Preventive Medicine,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"3389","DOI":"10.1093\/nar\/25.17.3389","article-title":"Gapped BLAST and PSI-BLAST: a new generation of protein search programs","volume":"25","author":"Altschul S. 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