{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T03:25:17Z","timestamp":1791343517949,"version":"4.3.1"},"reference-count":47,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2001,1,15]],"date-time":"2001-01-15T00:00:00Z","timestamp":979516800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2001,1,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Analysis of a<jats:italic>Brucella suis<\/jats:italic>1330 gene fused to a<jats:italic>gfp<\/jats:italic>reporter, and identified as being induced in J774 murine macrophage-like cells, allowed the isolation of a gene homologous to<jats:italic>nikA<\/jats:italic>, the first gene of the<jats:italic>Escherichia coli<\/jats:italic>operon encoding the specific transport system for nickel. DNA sequence analysis of the corresponding<jats:italic>B. suis nik<\/jats:italic>locus showed that it was highly similar to that of<jats:italic>E. coli<\/jats:italic>except for localization of the<jats:italic>nikR<\/jats:italic>regulatory gene, which lies upstream from the structural<jats:italic>nikABCDE<\/jats:italic>genes and in the opposite orientation. Protein sequence comparisons suggested that the deduced<jats:italic>nikABCDE<\/jats:italic>gene products belong to a periplasmic binding protein-dependent transport system. The<jats:italic>nikA<\/jats:italic>promoter-<jats:italic>gfp<\/jats:italic>fusion was activated in vitro by low oxygen tension and metal ion deficiency and was repressed by NiCl<jats:sub>2<\/jats:sub>excess. Insertional inactivation of<jats:italic>nikA<\/jats:italic>strongly reduced the activity of the nickel metalloenzyme urease, which was restored by addition of a nickel excess. Moreover, the<jats:italic>nikA<\/jats:italic>mutant of<jats:italic>B. suis<\/jats:italic>was functionally complemented with the<jats:italic>E. coli nik<\/jats:italic>gene cluster, leading to the recovery of urease activity. Reciprocally, an<jats:italic>E. coli<\/jats:italic>strain harboring a deleted<jats:italic>nik<\/jats:italic>operon recovered hydrogenase activity by heterologous complementation with the<jats:italic>B. suis nik<\/jats:italic>locus. Taking into account these results, we propose that the<jats:italic>nik<\/jats:italic>locus of<jats:italic>B. suis<\/jats:italic>encodes a nickel transport system. The results further suggest that nickel could enter<jats:italic>B. suis<\/jats:italic>via other transport systems. Intracellular growth rates of the<jats:italic>B. suis<\/jats:italic>wild-type and<jats:italic>nikA<\/jats:italic>mutant strains in human monocytes were similar, indicating that<jats:italic>nikA<\/jats:italic>was not essential for this step of infection. We discuss a possible role of nickel transport in maintaining enzymatic activities which could be crucial for survival of the bacteria under the environmental conditions encountered within the host.<\/jats:p>","DOI":"10.1128\/jb.183.2.426-434.2001","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T10:01:09Z","timestamp":1027764069000},"page":"426-434","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":56,"title":["Identification of the<i>nik<\/i>Gene Cluster of<i>Brucella suis<\/i>: Regulation and Contribution to Urease Activity"],"prefix":"10.1128","volume":"183","author":[{"given":"Ve\u0301ronique","family":"Jubier-Maurin","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale U-431, Institut E. Bataillon, Universite\u0301Montpellier II, 34095 Montpellier,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agne\u0300s","family":"Rodrigue","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Unite\u0301 de Microbiologie et Ge\u0301ne\u0301tique CNRS ERS 2009, INSA, 69621 Villeurbanne Cedex,2France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Safia","family":"Ouahrani-Bettache","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale U-431, Institut E. Bataillon, Universite\u0301Montpellier II, 34095 Montpellier,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marion","family":"Layssac","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale U-431, Institut E. Bataillon, Universite\u0301Montpellier II, 34095 Montpellier,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie-Andre\u0301e","family":"Mandrand-Berthelot","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Unite\u0301 de Microbiologie et Ge\u0301ne\u0301tique CNRS ERS 2009, INSA, 69621 Villeurbanne Cedex,2France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephan","family":"Ko\u0308hler","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale U-431, Institut E. Bataillon, Universite\u0301Montpellier II, 34095 Montpellier,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Pierre","family":"Liautard","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale U-431, Institut E. Bataillon, Universite\u0301Montpellier II, 34095 Montpellier,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"e_1_3_2_3_2","unstructured":"Ausubel F. M. Brent R. Kingston R. E. Moore D. D. Seidman J. G. Smith J. A. Struhl K. Current protocols in molecular biology. 1989 John Wiley & Sons New York N.Y"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.64.7.2877-2880.1996"},{"key":"e_1_3_2_5_2","doi-asserted-by":"crossref","first-page":"2885","DOI":"10.4049\/jimmunol.156.8.2885","article-title":"Brucella species release a specific, protease-sensitive, inhibitor of TNF-\u03b1 expression, active on human macrophage-like cells","volume":"156","author":"Caron E.","year":"1996","unstructured":"Caron E. Gross A. Liautard J.-P. Dornand J. Brucella species release a specific, protease-sensitive, inhibitor of TNF-\u03b1 expression, active on human macrophage-like cells.J. Immunol.156199628852893","journal-title":"J. Immunol."},{"key":"e_1_3_2_6_2","doi-asserted-by":"crossref","first-page":"2494","DOI":"10.1110\/ps.8.11.2494","article-title":"NikR is a ribbon-helix-helix DNA-binding protein","volume":"8","author":"Chivers P. T.","year":"1999","unstructured":"Chivers P. T. Sauer R. T. NikR is a ribbon-helix-helix DNA-binding protein.Protein Sci.8199924942500","journal-title":"Protein Sci."},{"key":"e_1_3_2_7_2","doi-asserted-by":"crossref","first-page":"19735","DOI":"10.1074\/jbc.M002232200","article-title":"Regulation of high affinity nickel uptake in bacteria. Ni2+-dependent interaction of NikR with wild-type and mutant operator sites","volume":"275","author":"Chivers P. T.","year":"2000","unstructured":"Chivers P. T. Sauer R. T. Regulation of high affinity nickel uptake in bacteria. Ni2+-dependent interaction of NikR with wild-type and mutant operator sites.J. Biol. Chem.27520001973519741","journal-title":"J. Biol. Chem."},{"key":"e_1_3_2_8_2","first-page":"339","article-title":"Brucella","volume":"2","author":"Corbel M. J.","year":"1990","unstructured":"Corbel M. J. Brucella Principles of bacteriology, virology, and immunity Parker M. T. Collier L. H. 2. E 1990 339 353 Arnold London, England","journal-title":"Principles of bacteriology, virology, and immunity"},{"key":"e_1_3_2_9_2","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/0003-2697(91)90041-Q","article-title":"\u201cPocket blotting\u201d: a method for transferring nucleic acids onto nylon membranes","volume":"193","author":"Cuny G.","year":"1991","unstructured":"Cuny G. Veas F. Roiz\u00e8s G. \u201cPocket blotting\u201d: a method for transferring nucleic acids onto nylon membranes.Anal. Biochem.19319914548","journal-title":"Anal. Biochem."},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.181.2.670-674.1999"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.59.12.4310-4317.1991"},{"key":"e_1_3_2_12_2","doi-asserted-by":"crossref","first-page":"1605","DOI":"10.1099\/00221287-146-7-1605","article-title":"Functional and analysis of the ClpATPase ClpA of Brucella suis, and persistence of a knockout mutant in BALB\/c mice","volume":"146","author":"Ekaza E.","year":"2000","unstructured":"Ekaza E. Guilloteau L. Teyssier J. Liautard J.-P. K\u00f6hler S. Functional and analysis of the ClpATPase ClpA of Brucella suis, and persistence of a knockout mutant in BALB\/c mice.Microbiology146200016051616","journal-title":"Microbiology"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.62.10.4135-4139.1994"},{"key":"e_1_3_2_14_2","doi-asserted-by":"crossref","first-page":"1297","DOI":"10.1128\/IAI.68.3.1297-1303.2000","article-title":"Identification of Brucella suis genes affecting intracellular survival in an in vitro human macrophage infection model by signature-tagged transposon mutagenesis","volume":"68","author":"Foulongne V.","year":"2000","unstructured":"Foulongne V. Bourg G. Cazevieille C. Michaux-Charachon S. O'Callaghan D. Identification of Brucella suis genes affecting intracellular survival in an in vitro human macrophage infection model by signature-tagged transposon mutagenesis.Infect. Immun.68200012971303","journal-title":"Infect. Immun."},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.164.2.918-921.1985"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.153.3.1424-1431.1983"},{"key":"e_1_3_2_17_2","first-page":"681","article-title":"SOPMA: significant improvement in protein secondary structure prediction by consensus prediction from multiple alignments","volume":"11","author":"Geourjon C.","year":"1995","unstructured":"Geourjon C. Del\u00e9age G. SOPMA: significant improvement in protein secondary structure prediction by consensus prediction from multiple alignments.Comput. Appl. Biosci.111995681684","journal-title":"Comput. Appl. Biosci."},{"key":"e_1_3_2_18_2","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1016\/0167-4781(92)90476-G","article-title":"Cloning and nucleotide sequence of the Brucella abortus groE operon","volume":"1130","author":"Gor D.","year":"1992","unstructured":"Gor D. Mayfield J. E. Cloning and nucleotide sequence of the Brucella abortus groE operon.Biochim. Biophys. Acta11301992120122","journal-title":"Biochim. Biophys. Acta"},{"key":"e_1_3_2_19_2","doi-asserted-by":"crossref","first-page":"942","DOI":"10.1128\/IAI.68.2.942-947.2000","article-title":"Construction of urease-negative mutants of Yersinia enterocolitica serotypes O:3 and O:8: role of urease in virulence and arthritogenicity","volume":"68","author":"Gripenberg-Lerche C.","year":"2000","unstructured":"Gripenberg-Lerche C. Zhang L. Ahtonen P. Toivanen P. Skurnik M. Construction of urease-negative mutants of Yersinia enterocolitica serotypes O:3 and O:8: role of urease in virulence and arthritogenicity.Infect. Immun.682000942947","journal-title":"Infect. Immun."},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.4.1309-1316.1998"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/mr.51.1.22-42.1987"},{"key":"e_1_3_2_22_2","doi-asserted-by":"crossref","first-page":"18729","DOI":"10.1016\/S0021-9258(17)30569-0","article-title":"Transcriptional regulation of hydrogenase synthesis by nickel in Bradyrhizobium japonicum","volume":"265","author":"Kim H.","year":"1990","unstructured":"Kim H. Maier R. J. Transcriptional regulation of hydrogenase synthesis by nickel in Bradyrhizobium japonicum.J. Biol. Chem.26519901872918732","journal-title":"J. Biol. Chem."},{"key":"e_1_3_2_23_2","doi-asserted-by":"crossref","first-page":"6695","DOI":"10.1128\/IAI.67.12.6695-6697.1999","article-title":"Constitutive and inducible expression of green fluorescent protein in Brucella suis","volume":"67","author":"K\u00f6hler S.","year":"1999","unstructured":"K\u00f6hler S. Ouahrani-Bettache S. Layssac M. Teyssier J. Liautard J.-P. Constitutive and inducible expression of green fluorescent protein in Brucella suis.Infect. Immun.67199966956697","journal-title":"Infect. Immun."},{"key":"e_1_3_2_24_2","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1111\/j.1365-2958.1996.tb02510.x","article-title":"Participation of the molecular chaperone DnaK in intracellular growth of Brucella suis within U937-derived phagocytes","volume":"20","author":"K\u00f6hler S.","year":"1996","unstructured":"K\u00f6hler S. Teyssier J. Cloeckaert A. Rouot B. Liautard J.-P. Participation of the molecular chaperone DnaK in intracellular growth of Brucella suis within U937-derived phagocytes.Mol. Microbiol.201996701712","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_25_2","first-page":"800","article-title":"pBBR1MCS: a broad-host-range cloning vector","volume":"16","author":"Kovach M. E.","year":"1994","unstructured":"Kovach M. E. Phillips R. W. Elzer P. H. Roop R. M. II Peterson K. M. pBBR1MCS: a broad-host-range cloning vector.BioTechniques161994800802","journal-title":"BioTechniques"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0923-2508(97)87645-0"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.60.6.2425-2431.1992"},{"key":"e_1_3_2_28_2","doi-asserted-by":"crossref","first-page":"539","DOI":"10.1007\/BF00285277","article-title":"A 4.6 kb DNA region of Rhizobium meliloti involved in determining urease and hydrogenase activities carries the structural genes for urease (ureaA, ureB, ureC) interrupted by other open reading frames","volume":"242","author":"Miksch G.","year":"1994","unstructured":"Miksch G. Arnold W. Lentzsch P. Priefer U. B. P\u00fchler A. A 4.6 kb DNA region of Rhizobium meliloti involved in determining urease and hydrogenase activities carries the structural genes for urease (ureaA, ureB, ureC) interrupted by other open reading frames.Mol. Gen. Genet.2421994539550","journal-title":"Mol. Gen. Genet."},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/mr.59.3.451-480.1995"},{"key":"e_1_3_2_30_2","first-page":"11","article-title":"Optimal alignments in linear space","volume":"4","author":"Myers E. W.","year":"1988","unstructured":"Myers E. W. Miller W. Optimal alignments in linear space.Comput. Appl. Biosci.419881117","journal-title":"Comput. Appl. Biosci."},{"key":"e_1_3_2_31_2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1574-6968.1997.tb12744.x","article-title":"Adaptation of Bacillus subtilis to oxygen limitation","volume":"157","author":"Nakano M. M.","year":"1997","unstructured":"Nakano M. M. Hulett F. M. Adaptation of Bacillus subtilis to oxygen limitation.FEMS Lett.157199717","journal-title":"FEMS Lett."},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.178.13.3796-3802.1996"},{"key":"e_1_3_2_33_2","unstructured":"Nash P. Krenz M. M. Culture media Manual of clinical microbiology 5th ed. Balows A. 1991 1280 American Society for Microbiology Washington D.C."},{"key":"e_1_3_2_34_2","doi-asserted-by":"crossref","first-page":"1181","DOI":"10.1111\/j.1365-2958.1993.tb01247.x","article-title":"The nik operon of Escherichia coli encodes a periplasmic binding-protein-dependent transport system for nickel","volume":"9","author":"Navarro C.","year":"1993","unstructured":"Navarro C. Wu L.-F. Mandrand-Berthelot M.-A. The nik operon of Escherichia coli encodes a periplasmic binding-protein-dependent transport system for nickel.Mol. Microbiol.9199311811191","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1999.01569.x"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.5.2387-2392.1998"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.8.4041-4047.1999"},{"key":"e_1_3_2_38_2","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1046\/j.1365-2958.2000.01726.x","article-title":"The Brucella abortus Lon functions as a generalized stress response protease and is required for wild-type virulence in BALB\/c mice","volume":"35","author":"Robertson G. T.","year":"2000","unstructured":"Robertson G. T. Kovach M. E. Allen C. A. Ficht T. A. Roop R. M. II. The Brucella abortus Lon functions as a generalized stress response protease and is required for wild-type virulence in BALB\/c mice.Mol. Microbiol.352000577588","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_39_2","doi-asserted-by":"crossref","first-page":"690","DOI":"10.1046\/j.1365-2958.1999.01629.x","article-title":"The Brucella abortus host factor I (HF-I) protein contributes to stress resistance during stationary phase and is a major determinant of virulence in mice","volume":"34","author":"Robertson G. T.","year":"1999","unstructured":"Robertson G. T. Roop R. M. II. The Brucella abortus host factor I (HF-I) protein contributes to stress resistance during stationary phase and is a major determinant of virulence in mice.Mol. Microbiol.341999690700","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_40_2","unstructured":"Sambrook J. Fritsch E. F. Maniatis T. Molecular cloning: a laboratory manual 2nd ed. 1989 Cold Spring Harbor Laboratory Cold Spring Harbor N.Y"},{"key":"e_1_3_2_41_2","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1016\/S1369-5274(99)80032-0","article-title":"The aerobic\/anaerobic interface","volume":"2","author":"Sawers G.","year":"1999","unstructured":"Sawers G. The aerobic\/anaerobic interface.Curr. Opin. Microbiol.21999181187","journal-title":"Curr. Opin. Microbiol."},{"key":"e_1_3_2_42_2","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1046\/j.1365-2958.1998.00913.x","article-title":"A two-component regulatory system playing a critical role in plant pathogens and endosymbionts is present in Brucella abortus and controls cell invasion and virulence","volume":"29","author":"Sola-Landa A.","year":"1998","unstructured":"Sola-Landa A. Pizarro-Cerda J. Grillo M.-J. Moreno E. Moriyon I. Blasco J.-M. Gorvel J.-P. Lopez-Goni I. A two-component regulatory system playing a critical role in plant pathogens and endosymbionts is present in Brucella abortus and controls cell invasion and virulence.Mol. Microbiol.291998125138","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_43_2","unstructured":"Sonnhammer E. L. L. von Heijne G. Krogh A. A hidden Markov model for predicting transmembrane helices in protein sequences Proceedings of the Sixth International Conference on Intelligent Systems for Molecular Biology. Glasgow J. Littlejohn T. Major F. Lathrop R. Sankoff D. Sensen C. 1998 175 182 AAAI Press Menlo Park Calif"},{"key":"e_1_3_2_44_2","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1006\/jmbi.1998.2107","article-title":"Principles governing amino acid composition of integral membrane proteins: application to topology prediction","volume":"283","author":"Tusn\u00e1dy G. E.","year":"1998","unstructured":"Tusn\u00e1dy G. E. Simon I. Principles governing amino acid composition of integral membrane proteins: application to topology prediction.J. Mol. Biol.2831998489506","journal-title":"J. Mol. Biol."},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1982.tb01276.x"},{"key":"e_1_3_2_46_2","doi-asserted-by":"crossref","first-page":"9218","DOI":"10.1073\/pnas.91.20.9218","article-title":"Coaxial stacking of helixes enhances binding of oligoribonucleotides and improves predictions of RNA folding","volume":"91","author":"Walter A. E.","year":"1994","unstructured":"Walter A. E. Turner D. H. Kim J. Lyttle M. H. Muller P. Mathews D. H. Zuker M. Coaxial stacking of helixes enhances binding of oligoribonucleotides and improves predictions of RNA folding.Proc. Natl. Acad. Sci. USA91199492189222","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"e_1_3_2_47_2","doi-asserted-by":"crossref","first-page":"1709","DOI":"10.1111\/j.1365-2958.1989.tb00156.x","article-title":"Nickel deficiency gives rise to the defective hydrogenase phenotype of hydC and fnr mutants in Escherichia coli","volume":"3","author":"Wu L.-F.","year":"1989","unstructured":"Wu L.-F. Mandrand-Berthelot M.-A. Waugh R. Edmonds C. J. Holt S. E. Boxer D. H. Nickel deficiency gives rise to the defective hydrogenase phenotype of hydC and fnr mutants in Escherichia coli.Mol. Microbiol.3198917091718","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_48_2","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/0378-1119(91)90294-L","article-title":"The hydC region contains a multi-cistronic operon (nik) involved in nickel transport in Escherichia coli","volume":"107","author":"Wu L.-F.","year":"1991","unstructured":"Wu L.-F. Navarro C. Mandrand-Berthelot M.-A. The hydC region contains a multi-cistronic operon (nik) involved in nickel transport in Escherichia coli.Gene10719913742","journal-title":"Gene"}],"container-title":["Journal of Bacteriology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JB.183.2.426-434.2001","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JB.183.2.426-434.2001","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,4,23]],"date-time":"2023-04-23T15:22:57Z","timestamp":1682263377000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JB.183.2.426-434.2001"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,1,15]]},"references-count":47,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2001,1,15]]}},"alternative-id":["10.1128\/JB.183.2.426-434.2001"],"URL":"https:\/\/doi.org\/10.1128\/jb.183.2.426-434.2001","relation":{},"ISSN":["0021-9193","1098-5530"],"issn-type":[{"value":"0021-9193","type":"print"},{"value":"1098-5530","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,1,15]]},"assertion":[{"value":"2000-07-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2000-10-19","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-20","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}