{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T23:40:08Z","timestamp":1772667608413,"version":"3.50.1"},"reference-count":37,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2001,3,1]],"date-time":"2001-03-01T00:00:00Z","timestamp":983404800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2001,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The\n            <jats:italic>psbAI<\/jats:italic>\n            gene of the cyanobacterium\n            <jats:italic>Synechococcus elongatus<\/jats:italic>\n            PCC 7942 is one of three\n            <jats:italic>psbA<\/jats:italic>\n            genes that encode a critical photosystem II reaction center protein, D1. Regulation of the gene family in response to changes in the light environment is complex, occurs at transcriptional and posttranscriptional levels, and results in an interchange of two different forms of D1 in the membrane. Expression of\n            <jats:italic>psbAI<\/jats:italic>\n            is downregulated under high-intensity light (high light) in contrast to induction of the other two family members. We show that, in addition to a known accelerated degradation of the\n            <jats:italic>psbAI<\/jats:italic>\n            message, promoter activity decreases upon exposure to high light. Unlike the other\n            <jats:italic>psbA<\/jats:italic>\n            genes, additional sequences upstream of the\n            <jats:italic>psbAI<\/jats:italic>\n            \u221235 element are required for expression. Mutagenizing the atypical\n            <jats:italic>psbAI<\/jats:italic>\n            \u221210 element from TCTCCT to TATAAT increased the magnitude of expression from both\n            <jats:italic>psbAI<\/jats:italic>\n            ::\n            <jats:italic>lacZ<\/jats:italic>\n            and\n            <jats:italic>psbAI<\/jats:italic>\n            ::\n            <jats:italic>luxAB<\/jats:italic>\n            fusions but did not affect downregulation under high light. Inactivation of group 2 sigma factor genes\n            <jats:italic>rpoD2<\/jats:italic>\n            and\n            <jats:italic>sigC<\/jats:italic>\n            , in both wild-type and \u221210-element mutagenized backgrounds, resulted in elevated\n            <jats:italic>psbAI<\/jats:italic>\n            ::\n            <jats:italic>luxAB<\/jats:italic>\n            expression but did not alter the response to high light. The results are consistent with redundancy of promoter recognition among cyanobacterial group 2 sigma factors. Electrophoretic mobility shift assays showed that the DNA sequence corresponding to the untranslated leader of the\n            <jats:italic>psbAI<\/jats:italic>\n            message binds one or more proteins from an\n            <jats:italic>S. elongatus<\/jats:italic>\n            extract. The corresponding region of\n            <jats:italic>psbAII<\/jats:italic>\n            efficiently competed for this binding activity, suggesting a shared regulatory factor among these disparately regulated genes.\n          <\/jats:p>","DOI":"10.1128\/jb.183.5.1740-1747.2001","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T10:01:09Z","timestamp":1027764069000},"page":"1740-1747","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":46,"title":["Functional Elements of the Strong\n            <i>psbAI<\/i>\n            Promoter of\n            <i>Synechococcus elongatus<\/i>\n            PCC 7942"],"prefix":"10.1128","volume":"183","author":[{"given":"Usha","family":"Nair","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Department of Biology, Texas A&amp;M University, College Station, Texas 77843-3258"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Colleen","family":"Thomas","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Biology, Texas A&amp;M University, College Station, Texas 77843-3258"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susan S.","family":"Golden","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Biology, Texas A&amp;M University, College Station, Texas 77843-3258"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1016\/S0076-6879(00)05511-7","article-title":"Application of bioluminescence to the study of circadian rhythms in cyanobacteria","volume":"305","author":"Andersson C. 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