{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T21:59:41Z","timestamp":1791064781101,"version":"4.1.0"},"reference-count":39,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2001,3,15]],"date-time":"2001-03-15T00:00:00Z","timestamp":984614400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2001,3,15]]},"abstract":"ABSTRACT<\/jats:title>\n \n \n rsmB\n Ecc<\/jats:sub>\n <\/jats:italic>\n specifies a nontranslatable RNA regulator that controls exoprotein production and pathogenicity in soft rot-causing\n Erwinia carotovora<\/jats:italic>\n subsp.\n carotovora<\/jats:italic>\n . This effect of\n \n rsmB\n Ecc<\/jats:sub>\n <\/jats:italic>\n RNA is mediated mostly by neutralizing the function of RsmA\n Ecc<\/jats:sub>\n , an RNA-binding protein of\n E. carotovora<\/jats:italic>\n subsp.\n carotovora<\/jats:italic>\n , which acts as a global negative regulator. To determine the occurrence of functional homologs of\n \n rsmB\n Ecc<\/jats:sub>\n <\/jats:italic>\n in non-soft-rot-causing\n Erwinia<\/jats:italic>\n species, we cloned the\n rsmB<\/jats:italic>\n genes of\n E. amylovora<\/jats:italic>\n (\n \n rsmB\n Ea<\/jats:sub>\n <\/jats:italic>\n ) and\n E. herbicola<\/jats:italic>\n pv. gypsophilae (\n \n rsmB\n Ehg<\/jats:sub>\n <\/jats:italic>\n ). We show that\n \n rsmB\n Ea<\/jats:sub>\n <\/jats:italic>\n in\n E. amylovora<\/jats:italic>\n positively regulates extracellular polysaccharide (EPS) production, motility, and pathogenicity. In\n E. herbicola<\/jats:italic>\n pv. gypsophilae,\n \n rsmB\n Ehg<\/jats:sub>\n <\/jats:italic>\n elevates the levels of transcripts of a cytokinin (\n etz<\/jats:italic>\n ) gene and stimulates the production of EPS and yellow pigment as well as motility. RsmA\n Ea<\/jats:sub>\n and RsmA\n Ehg<\/jats:sub>\n have more than 93% identity to RsmA\n Ecc<\/jats:sub>\n and, like the latter, function as negative regulators by affecting the transcript stability of the target gene. The\n rsmB<\/jats:italic>\n genes reverse the negative effects of RsmA\n Ea<\/jats:sub>\n , RsmA\n Ehg<\/jats:sub>\n , and RsmA\n Ecc<\/jats:sub>\n , but the extent of reversal is highest with homologous combinations of\n rsm<\/jats:italic>\n genes. These observations and findings that\n \n rsmB\n Ea<\/jats:sub>\n <\/jats:italic>\n and\n \n rsmB\n Ehg<\/jats:sub>\n <\/jats:italic>\n RNA bind RsmA\n Ecc<\/jats:sub>\n indicate that the\n rsmB<\/jats:italic>\n effect is channeled via RsmA. Additional support for this conclusion comes from the observation that the\n rsmB<\/jats:italic>\n genes are much more effective as positive regulators in a RsmA\n +<\/jats:sup>\n strain of\n E. carotovora<\/jats:italic>\n subsp.\n carotovora<\/jats:italic>\n than in its RsmA\n \u2212<\/jats:sup>\n derivative.\n E. herbicola<\/jats:italic>\n pv. gypsophilae produces a 290-base\n rsmB<\/jats:italic>\n transcript that is not subject to processing. By contrast,\n E. amylovora<\/jats:italic>\n produces 430- and 300-base\n rsmB<\/jats:italic>\n transcripts, the latter presumably derived by processing of the primary transcript as previously noted with the transcripts of\n \n rsmB\n Ecc<\/jats:sub>\n <\/jats:italic>\n . Southern blot hybridizations revealed the presence of\n rsmB<\/jats:italic>\n homologs in\n E. carotovora, E. chrysanthemi, E. amylovora, E. herbicola, E. stewartii<\/jats:italic>\n and\n E. rhapontici<\/jats:italic>\n , as well as in other enterobacteria such as\n Escherichia coli, Salmonella enterica<\/jats:italic>\n serovar Typhimurium,\n Serratia marcescens, Shigella flexneri, Enterobacter aerogenes, Klebsiella pneumoniae, Yersinia enterocolitica<\/jats:italic>\n , and\n Y. pseudotuberculosis<\/jats:italic>\n . A comparison of\n rsmB<\/jats:italic>\n sequences from several of these enterobacterial species revealed a highly conserved 34-mer region which is predicted to play a role in positive regulation by\n rsmB<\/jats:italic>\n RNA.\n <\/jats:p>","DOI":"10.1128\/jb.183.6.1870-1880.2001","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T10:01:09Z","timestamp":1027764069000},"page":"1870-1880","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":41,"title":["Molecular Characterization of Global Regulatory RNA Species That Control Pathogenicity Factors in\n Erwinia amylovora<\/i>\n and\n Erwinia herbicola<\/i>\n pv. gypsophilae"],"prefix":"10.1128","volume":"183","author":[{"given":"Weilei","family":"Ma","sequence":"first","affiliation":[{"name":"Department of Plant Microbiology and Pathology, Plant Sciences Unit, University of Missouri, Columbia, Missouri 65211"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaya","family":"Cui","sequence":"additional","affiliation":[{"name":"Department of Plant Microbiology and Pathology, Plant Sciences Unit, University of Missouri, Columbia, Missouri 65211"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Plant Microbiology and Pathology, Plant Sciences Unit, University of Missouri, Columbia, Missouri 65211"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. Korsi","family":"Dumenyo","sequence":"additional","affiliation":[{"name":"Department of Plant Microbiology and Pathology, Plant Sciences Unit, University of Missouri, Columbia, Missouri 65211"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asita","family":"Mukherjee","sequence":"additional","affiliation":[{"name":"Department of Plant Microbiology and Pathology, Plant Sciences Unit, University of Missouri, Columbia, Missouri 65211"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arun K.","family":"Chatterjee","sequence":"additional","affiliation":[{"name":"Department of Plant Microbiology and Pathology, Plant Sciences Unit, University of Missouri, Columbia, Missouri 65211"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"111905","DOI":"10.1016\/S0021-9258(19)68491-7","article-title":"Evidence for two functional gal promoters in intact Escherichia coli","volume":"256","author":"Aiba H.","year":"1981","unstructured":"Aiba H. 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