{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T19:32:58Z","timestamp":1791142378249,"version":"4.1.0"},"reference-count":22,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2001,3,15]],"date-time":"2001-03-15T00:00:00Z","timestamp":984614400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2001,3,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Protective antigen (PA), a component of anthrax toxin, binds receptors on mammalian cells and is activated by a cell surface protease. The resulting active fragment, PA\n            <jats:sub>63<\/jats:sub>\n            , forms ring-shaped heptamers, binds the enzymic moieties of the toxin, and translocates them to the cytosol. Of the four crystallographic domains of PA, domain 1 has been implicated in binding the enzymic moieties; domain 2 is involved in membrane insertion and oligomerization; and domain 4 binds receptor. To determine the function of domain 3, we developed a screen that allowed us to isolate random mutations that cause defects in the activity of PA. We identified several mutations in domain 3 that affect monomer-monomer interactions in the PA\n            <jats:sub>63<\/jats:sub>\n            heptamer, indicating that this may be the primary function of this domain.\n          <\/jats:p>","DOI":"10.1128\/jb.183.6.2111-2116.2001","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T10:01:09Z","timestamp":1027764069000},"page":"2111-2116","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":74,"title":["Involvement of Domain 3 in Oligomerization by the Protective Antigen Moiety of Anthrax Toxin"],"prefix":"10.1128","volume":"183","author":[{"given":"Jeremy","family":"Mogridge","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Mourez","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. John","family":"Collier","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"3941","DOI":"10.1021\/bi972657b","article-title":"Identification of residues lining the anthrax protective antigen channel","volume":"37","author":"Benson E. L.","year":"1998","unstructured":"Benson E. L. Huynh P. D. Finkelstein A. Collier R. J. Identification of residues lining the anthrax protective antigen channel.Biochemistry37199839413948","journal-title":"Biochemistry"},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"2209","DOI":"10.1073\/pnas.86.7.2209","article-title":"Anthrax toxin: channel-forming activity of protective antigen in planar phospholipid bilayers","volume":"86","author":"Blaustein R. O.","year":"1989","unstructured":"Blaustein R. O. Koehler T. M. Collier R. J. Finkelstein A. Anthrax toxin: channel-forming activity of protective antigen in planar phospholipid bilayers.Proc. Natl. Acad. Sci. USA86198922092213","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"e_1_3_2_4_2","doi-asserted-by":"crossref","first-page":"964","DOI":"10.1128\/IAI.67.2.964-967.1999","article-title":"Functional analysis of the carboxy-terminal domain of Bacillus anthracis protective antigen","volume":"67","author":"Brossier F.","year":"1999","unstructured":"Brossier F. Sirard J. Guidi-Rontani C. Duflot E. Mock M. Functional analysis of the carboxy-terminal domain of Bacillus anthracis protective antigen.Infect. Immun.671999964967","journal-title":"Infect. 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