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In the attempt to characterize the stress response in\n            <jats:italic>Brucella suis<\/jats:italic>\n            , a gene highly homologous to\n            <jats:italic>Escherichia coli clpB<\/jats:italic>\n            was isolated from\n            <jats:italic>Brucella suis<\/jats:italic>\n            , and the deduced amino acid sequence showed features typical of the ClpB ATPase family of stress response proteins. Under high-temperature stress conditions, ClpB of\n            <jats:italic>B. suis<\/jats:italic>\n            was induced, and an isogenic\n            <jats:italic>B. suis clpB<\/jats:italic>\n            mutant showed increased sensitivity to high temperature, but also to ethanol stress and acid pH. The effects were reversible by complementation. Simultaneous inactivation of\n            <jats:italic>clpA<\/jats:italic>\n            and\n            <jats:italic>clpB<\/jats:italic>\n            resulted in a mutant that was sensitive to oxidative stress. In\n            <jats:italic>B. suis<\/jats:italic>\n            expressing\n            <jats:italic>gfp<\/jats:italic>\n            , ClpA but not ClpB participated in degradation of the green fluorescent protein at 42\u00b0C. We concluded that ClpB was responsible for tolerance to several stresses and that the lethality caused by harsh environmental conditions may have similar molecular origins.\n          <\/jats:p>","DOI":"10.1128\/jb.183.8.2677-2681.2001","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T10:01:09Z","timestamp":1027764069000},"page":"2677-2681","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":43,"title":["Characterization of\n            <i>Brucella suis clpB<\/i>\n            and\n            <i>clpAB<\/i>\n            Mutants and Participation of the Genes in Stress Responses"],"prefix":"10.1128","volume":"183","author":[{"given":"Euloge","family":"Ekaza","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale U-431, Universite\u0301 Montpellier II, F-34095 Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacques","family":"Teyssier","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale U-431, Universite\u0301 Montpellier II, F-34095 Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Safia","family":"Ouahrani-Bettache","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale U-431, Universite\u0301 Montpellier II, F-34095 Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Pierre","family":"Liautard","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale U-431, Universite\u0301 Montpellier II, F-34095 Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephan","family":"Ko\u0308hler","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Institut National de la Sante\u0301 et de la Recherche Me\u0301dicale U-431, Universite\u0301 Montpellier II, F-34095 Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.180.2.426-429.1998"},{"key":"e_1_3_2_3_2","first-page":"339","article-title":"Brucella","volume":"2","author":"Corbel M. 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