{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T03:27:26Z","timestamp":1772162846783,"version":"3.50.1"},"reference-count":28,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2002,5,15]],"date-time":"2002-05-15T00:00:00Z","timestamp":1021420800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2002,5,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The RcsCB His-Asp phosphorelay system regulates the expression of several genes of\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    , but the molecular nature of the inducing signal is still unknown. We show here that treatment of an exponentially growing culture of\n                    <jats:italic>E. coli<\/jats:italic>\n                    with the cationic amphipathic compound chlorpromazine (CPZ) stimulates expression of a set of genes positively regulated by the RcsCB system. This induction is abolished in\n                    <jats:italic>rcsB<\/jats:italic>\n                    or\n                    <jats:italic>rcsC<\/jats:italic>\n                    mutant strains. In addition, treatment with CPZ inhibits growth. The wild-type strain is able to recover from this inhibition and resume growth after a period of adaptation. In contrast, strains deficient in the RcsCB His-Asp phosphorelay system are hypersensitive to CPZ. These results suggest that cells must express specific RcsCB-regulated genes in order to cope with the CPZ-induced stress. This is the first report of the essential role of the RcsCB system in a stress situation. These results also strengthen the notion that alterations of the cell envelope induce a signal recognized by the RcsC sensor.\n                  <\/jats:p>","DOI":"10.1128\/jb.184.10.2850-2853.2002","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T06:09:29Z","timestamp":1027750169000},"page":"2850-2853","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":42,"title":["The RcsCB His-Asp Phosphorelay System Is Essential To Overcome Chlorpromazine-Induced Stress in\n                    <i>Escherichia coli<\/i>"],"prefix":"10.1128","volume":"184","author":[{"given":"Annie","family":"Conter","sequence":"first","affiliation":[{"name":"Laboratoire de Microbiologie et de Ge\u0301ne\u0301tique Mole\u0301culaire, Centre National de la Recherche Scientifique, 31062 Toulouse, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rachel","family":"Sturny","sequence":"additional","affiliation":[{"name":"Laboratoire de Microbiologie et de Ge\u0301ne\u0301tique Mole\u0301culaire, Centre National de la Recherche Scientifique, 31062 Toulouse, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claude","family":"Gutierrez","sequence":"additional","affiliation":[{"name":"Laboratoire de Microbiologie et de Ge\u0301ne\u0301tique Mole\u0301culaire, Centre National de la Recherche Scientifique, 31062 Toulouse, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaymeuang","family":"Cam","sequence":"additional","affiliation":[{"name":"Laboratoire de Microbiologie et de Ge\u0301ne\u0301tique Mole\u0301culaire, Centre National de la Recherche Scientifique, 31062 Toulouse, France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"19","volume":"1","year":"1988","unstructured":"Allen, P. 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