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Among the different<jats:italic>Brucella<\/jats:italic>species,<jats:italic>Brucella melitensis<\/jats:italic>is considered the most virulent. Despite successful use in animals, the vaccine strains remain infectious for humans. To understand the mechanism of virulence in<jats:italic>B. melitensis<\/jats:italic>, the proteome of vaccine strain Rev 1 was analyzed by two-dimensional gel electrophoresis and compared to that of virulent strain 16M. The two strains were grown under identical laboratory conditions. Computer-assisted analysis of the two<jats:italic>B. melitensis<\/jats:italic>proteomes revealed proteins expressed in either 16M or Rev 1, as well as up- or down-regulation of proteins specific for each of these strains. These proteins were identified by peptide mass fingerprinting. It was found that certain metabolic pathways may be deregulated in Rev 1. Expression of an immunogenic 31-kDa outer membrane protein, proteins utilized for iron acquisition, and those that play a role in sugar binding, lipid degradation, and amino acid binding was altered in Rev 1.<\/jats:p>","DOI":"10.1128\/jb.184.18.4962-4970.2002","type":"journal-article","created":{"date-parts":[[2002,10,1]],"date-time":"2002-10-01T19:36:19Z","timestamp":1033500979000},"page":"4962-4970","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":56,"title":["Comparative Proteome Analysis of<i>Brucella melitensis<\/i>Vaccine Strain Rev 1 and a Virulent Strain, 16M"],"prefix":"10.1128","volume":"184","author":[{"given":"Michel","family":"Eschenbrenner","sequence":"first","affiliation":[{"name":"Institute of Molecular Biology and Medicine, The University of Scranton, Scranton, Pennsylvania 18510"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary Ann","family":"Wagner","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biology and Medicine, The University of Scranton, Scranton, Pennsylvania 18510"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Troy A.","family":"Horn","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biology and Medicine, The University of Scranton, Scranton, Pennsylvania 18510"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo Ann","family":"Kraycer","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biology and Medicine, The University of Scranton, Scranton, Pennsylvania 18510"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cesar V.","family":"Mujer","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biology and Medicine, The University of Scranton, Scranton, Pennsylvania 18510"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sue","family":"Hagius","sequence":"additional","affiliation":[{"name":"Department of Veterinary Science, Louisiana State University AgCenter, Baton Rouge, Louisiana 70803"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip","family":"Elzer","sequence":"additional","affiliation":[{"name":"Department of Veterinary Science, Louisiana State University AgCenter, Baton Rouge, Louisiana 70803"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vito G.","family":"DelVecchio","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biology and Medicine, The University of Scranton, Scranton, Pennsylvania 18510"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.8.4816-4822.2001"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.10.6264-6270.2001"},{"key":"e_1_3_2_4_2","unstructured":"Alton G. 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