{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T05:01:35Z","timestamp":1791262895188,"version":"4.1.0"},"reference-count":53,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2002,2,1]],"date-time":"2002-02-01T00:00:00Z","timestamp":1012521600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2002,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Escherichia coli<\/jats:italic>\n            nucleoid-associated H-NS protein interacts with the Hha protein, a member of a new family of global modulators that also includes the YmoA protein from\n            <jats:italic>Yersinia enterocolitica<\/jats:italic>\n            . This interaction has been found to be involved in the regulation of the expression of the toxin \u03b1-hemolysin. In this study, we further characterize the interaction between H-NS and Hha. We show that the presence of DNA in preparations of copurified His-Hha and H-NS is not directly implicated in the interaction between the proteins. The precise molecular mass of the H-NS protein retained by Hha, obtained by mass spectrometry analysis, does not show any posttranslational modification other than removal of the N-terminal Met residue. We constructed an H-NS-His recombinant protein and found that, as expected, it interacts with Hha. We used a Ni\n            <jats:sup>2+<\/jats:sup>\n            -nitrilotriacetic acid agarose method for affinity chromatography copurification of proteins to identify the H-NS protein of\n            <jats:italic>Y. enterocolitica<\/jats:italic>\n            . We constructed a six-His-YmoA recombinant protein derived from YmoA, the homologue of Hha in\n            <jats:italic>Y. enterocolitica<\/jats:italic>\n            , and found that it interacts with\n            <jats:italic>Y. enterocolitica<\/jats:italic>\n            H-NS. We also cloned and sequenced the\n            <jats:italic>hns<\/jats:italic>\n            gene of this microorganism. In the course of these experiments we found that His-YmoA can also retain H-NS from\n            <jats:italic>E. coli<\/jats:italic>\n            . We also found that the\n            <jats:italic>hns<\/jats:italic>\n            gene of\n            <jats:italic>Y. enterocolitica<\/jats:italic>\n            can complement an\n            <jats:italic>hns<\/jats:italic>\n            mutation of\n            <jats:italic>E. coli<\/jats:italic>\n            . Finally, we describe for the first time systematic characterization of missense mutant alleles of\n            <jats:italic>hha<\/jats:italic>\n            and truncated Hha\u2032 proteins, and we report a striking and previously unnoticed similarity of the Hha family of proteins to the oligomerization domain of the H-NS proteins.\n          <\/jats:p>","DOI":"10.1128\/jb.184.3.629-635.2002","type":"journal-article","created":{"date-parts":[[2009,4,23]],"date-time":"2009-04-23T18:32:58Z","timestamp":1240511578000},"page":"629-635","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":98,"title":["Evidence for Direct Protein-Protein Interaction between Members of the Enterobacterial Hha\/YmoA and H-NS Families of Proteins"],"prefix":"10.1128","volume":"184","author":[{"given":"J. M.","family":"Nieto","sequence":"first","affiliation":[{"name":"Departament de Microbiologia, Facultat de Biologia, Universitat de Barcelona, 08028 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Madrid","sequence":"additional","affiliation":[{"name":"Departament de Microbiologia, Facultat de Biologia, Universitat de Barcelona, 08028 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Miquelay","sequence":"additional","affiliation":[{"name":"Departament de Microbiologia, Facultat de Biologia, Universitat de Barcelona, 08028 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. L.","family":"Parra","sequence":"additional","affiliation":[{"name":"Departament de Microbiologia, Facultat de Biologia, Universitat de Barcelona, 08028 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Rodri\u0301guez","sequence":"additional","affiliation":[{"name":"Departament de Microbiologia, Facultat de Biologia, Universitat de Barcelona, 08028 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Jua\u0301rez","sequence":"additional","affiliation":[{"name":"Departament de Microbiologia, Facultat de Biologia, Universitat de Barcelona, 08028 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1046\/j.1365-2958.1997.3151679.x","volume":"24","year":"1997","unstructured":"Atlung, T., and H. Ingmer. 1997. H-NS: a modulator of environmentally regulated gene expression. Mol. Microbiol.24:7\u201317.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.181.10.3018-3024.1999"},{"key":"e_1_3_2_4_2","doi-asserted-by":"crossref","first-page":"1841","DOI":"10.1099\/13500872-142-7-1841","volume":"142","year":"1996","unstructured":"Balsalobre, C., A. Ju\u00e1rez, C. Madrid, M. Mouri\u00f1o, A. Prenafeta, and F. Mu\u00f1oa. 1996. Complementation of the hha mutation in Escherichia coli by the ymoA gene from Yersinia enterocolitica: dependence on the gene dosage. Microbiology142:1841\u20131846.","journal-title":"Microbiology"},{"key":"e_1_3_2_5_2","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1046\/j.1365-2958.1999.01176.x","volume":"31","year":"1999","unstructured":"Bertin, P., N. Benhabiles, E. Krin, C. Laurent-Winter, C. Tendeng, E. Turlin, A. Thomas, A. Danchin, and R. Brasseur. 1999. 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