{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T01:40:41Z","timestamp":1790905241405,"version":"4.1.0"},"reference-count":38,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2002,2,1]],"date-time":"2002-02-01T00:00:00Z","timestamp":1012521600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2002,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            This study describes the characterization of\n            <jats:italic>orf358<\/jats:italic>\n            , an open reading frame of previously unidentified function, in the purple bacterium\n            <jats:italic>Rubrivivax gelatinosus<\/jats:italic>\n            . A strain in which\n            <jats:italic>orf358<\/jats:italic>\n            was disrupted exhibited a phenotype similar to the wild type under photosynthesis or low-aeration respiratory growth conditions. In contrast, under highly aerated respiratory growth conditions, the wild type still produced bacteriochlorophyll\n            <jats:italic>a<\/jats:italic>\n            (Bchl\n            <jats:italic>a<\/jats:italic>\n            ), while the disrupted strain accumulated a compound that had the same absorption and fluorescence emission spectra as Mg-protoporphyrin but was less polar, suggesting that it was Mg-protoporphyrin monomethylester (MgPMe). These data indicated a blockage in Bchl\n            <jats:italic>a<\/jats:italic>\n            synthesis at the oxidative cyclization stage and implied the coexistence of two different mechanisms for MgPMe cyclization in\n            <jats:italic>R. gelatinosus<\/jats:italic>\n            , an anaerobic mechanism active under photosynthesis or low oxygenation and an aerobic mechanism active under high-oxygenation growth conditions. Based on these results as well as on sequence analysis indicating the presence of conserved putative binuclear-iron-cluster binding motifs, the designation of\n            <jats:italic>orf358<\/jats:italic>\n            as\n            <jats:italic>acsF<\/jats:italic>\n            (for aerobic cyclization system Fe-containing subunit) is proposed. Several homologs of AcsF were found in a wide range of photosynthetic organisms, including\n            <jats:italic>Chlamydonomas reinhardtii<\/jats:italic>\n            Crd1 and\n            <jats:italic>Pharbitis nil<\/jats:italic>\n            PNZIP, suggesting that this aerobic oxidative cyclization mechanism is conserved from bacteria to plants.\n          <\/jats:p>","DOI":"10.1128\/jb.184.3.746-753.2002","type":"journal-article","created":{"date-parts":[[2009,4,23]],"date-time":"2009-04-23T18:32:58Z","timestamp":1240511578000},"page":"746-753","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":109,"title":["<i>Rubrivivax gelatinosus acsF<\/i>\n            (Previously\n            <i>orf358<\/i>\n            ) Codes for a Conserved, Putative Binuclear-Iron-Cluster-Containing Protein Involved in Aerobic Oxidative Cyclization of Mg-Protoporphyrin IX Monomethylester"],"prefix":"10.1128","volume":"184","author":[{"given":"Violaine","family":"Pinta","sequence":"first","affiliation":[{"name":"Laboratoire de Ge\u0301ne\u0301tique des Bacte\u0301ries Photosynthe\u0301tiques, Centre de Ge\u0301ne\u0301tique Mole\u0301culaire, UPR2167 associe\u0301e a\u0300 l\u2019Universite\u0301 Pierre-et-Marie-Curie, CNRS, 91198 Gif-sur-Yvette Cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martine","family":"Picaud","sequence":"additional","affiliation":[{"name":"Laboratoire de Ge\u0301ne\u0301tique des Bacte\u0301ries Photosynthe\u0301tiques, Centre de Ge\u0301ne\u0301tique Mole\u0301culaire, UPR2167 associe\u0301e a\u0300 l\u2019Universite\u0301 Pierre-et-Marie-Curie, CNRS, 91198 Gif-sur-Yvette Cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franc\u0327oise","family":"Reiss-Husson","sequence":"additional","affiliation":[{"name":"Laboratoire de Ge\u0301ne\u0301tique des Bacte\u0301ries Photosynthe\u0301tiques, Centre de Ge\u0301ne\u0301tique Mole\u0301culaire, UPR2167 associe\u0301e a\u0300 l\u2019Universite\u0301 Pierre-et-Marie-Curie, CNRS, 91198 Gif-sur-Yvette Cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chantal","family":"Astier","sequence":"additional","affiliation":[{"name":"Laboratoire de Ge\u0301ne\u0301tique des Bacte\u0301ries Photosynthe\u0301tiques, Centre de Ge\u0301ne\u0301tique Mole\u0301culaire, UPR2167 associe\u0301e a\u0300 l\u2019Universite\u0301 Pierre-et-Marie-Curie, CNRS, 91198 Gif-sur-Yvette Cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1007\/BF00034855","volume":"23","year":"1990","unstructured":"Agalidis, I., E. 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