{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T06:10:24Z","timestamp":1770358224775,"version":"3.49.0"},"reference-count":27,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2002,2,15]],"date-time":"2002-02-15T00:00:00Z","timestamp":1013731200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2002,2,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Subgenomic DNA microarrays were employed to evaluate the expression of the accessory gene regulator (\n            <jats:italic>agr<\/jats:italic>\n            locus) as well as multiple virulence-associated genes in\n            <jats:italic>Staphylococcus aureus<\/jats:italic>\n            . Gene expression was examined during growth of\n            <jats:italic>S. aureus<\/jats:italic>\n            in vitro in standard laboratory medium and rabbit serum and in vivo in subcutaneous chambers implanted in either nonimmune rabbits or rabbits immunized with staphylococcal enterotoxin B. Expression of RNAIII, the effector molecule of the\n            <jats:italic>agr<\/jats:italic>\n            locus, was dramatically repressed in serum and in vivo, despite the increased expression of secreted virulence factors sufficient to cause toxic shock syndrome (TSS) in the animals. Statistical analysis and clustering of virulence genes based on their expression profiles in the various experimental conditions demonstrated no positive correlation between the expression of\n            <jats:italic>agr<\/jats:italic>\n            and any staphylococcal virulence factors examined. Disruption of the\n            <jats:italic>agr<\/jats:italic>\n            locus had only a minimal effect on the expression in vivo of the virulence factors examined. An effect of immunization on the expression of\n            <jats:italic>agr<\/jats:italic>\n            and virulence factors was also observed. These results suggest that\n            <jats:italic>agr<\/jats:italic>\n            activation is not necessary for development of staphylococcal TSS and that regulatory circuits responding to the in vivo environment override\n            <jats:italic>agr<\/jats:italic>\n            activity.\n          <\/jats:p>","DOI":"10.1128\/jb.184.4.1095-1101.2002","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:55:53Z","timestamp":1027763753000},"page":"1095-1101","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":101,"title":["Repression of the\n            <i>Staphylococcus aureus<\/i>\n            Accessory Gene Regulator in Serum and In Vivo"],"prefix":"10.1128","volume":"184","author":[{"given":"Jeremy M.","family":"Yarwood","sequence":"first","affiliation":[{"name":"Department of Microbiology, Medical School, University of Minnesota, Minneapolis"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John K.","family":"McCormick","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Medical School, University of Minnesota, Minneapolis"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael L.","family":"Paustian","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathobiology and Biomedical Genomics Center, University of Minnesota, St. Paul, Minnesota"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vivek","family":"Kapur","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathobiology and Biomedical Genomics Center, University of Minnesota, St. Paul, Minnesota"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrick M.","family":"Schlievert","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Medical School, University of Minnesota, Minneapolis"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"2469","DOI":"10.1099\/00221287-144-9-2469","volume":"144","year":"1998","unstructured":"Chan, P. F., and S. J. Foster. 1998. The role of environmental factors in the regulation of virulence-determinant expression in Staphylococcus aureus 8325-4. Microbiology144:2469-2479.","journal-title":"Microbiology"},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"1815","DOI":"10.1172\/JCI117530","volume":"94","year":"1994","unstructured":"Cheung, A. L., K. J. Eberhardt, E. Chung, M. R. Yeaman, P. M. Sullam, M. Ramos, and A. S. Bayer. 1994. Diminished virulence of a sar\u2212\/agr\u2212 mutant of Staphylococcus aureus in the rabbit model of endocarditis. J. Clin. Investig.94:1815-1822.","journal-title":"J. Clin. Investig."},{"key":"e_1_3_2_4_2","first-page":"422","year":"2000","unstructured":"Collins, L. V., and A. Tarkowski. 2000. Animal models of experimental Staphylococcus aureus infection, p. 422-430. In V. A. Fischetti, R. P. Novick, J. J. Ferretti, D. A. Portnoy, and J. I. Rood (ed.), Gram-positive pathogens. ASM Press, Washington, D.C.","journal-title":"Gram-positive pathogens"},{"key":"e_1_3_2_5_2","doi-asserted-by":"crossref","first-page":"1301","DOI":"10.1099\/00221287-139-6-1301","volume":"139","year":"1993","unstructured":"Dassy, B., T. Hogan, T. J. Foster, and J. M. Fournier. 1993. Involvement of the accessory gene regulator (agr) in expression of type 5 capsular polysaccharide by Staphylococcus aureus. J. Gen. Microbiol.139:1301-1306.","journal-title":"J. Gen. Microbiol."},{"key":"e_1_3_2_6_2","first-page":"167","volume":"229","year":"1998","unstructured":"Dinges, M. M., J. Jessurun, and P. M. Schlievert. 1998. Comparisons of mouse and rabbit models of toxic shock syndrome. Int. Congr. Symp. Ser.229:167-168.","journal-title":"Int. Congr. Symp. Ser."},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.13.1.16"},{"key":"e_1_3_2_8_2","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1076\/ceyr.18.3.177.5370","volume":"18","year":"1999","unstructured":"Giese, M. J., J. A. Berliner, A. Riesner, E. A. Wagar, and B. J. Mondino. 1999. A comparison of the early inflammatory effects of an agr\u2212\/sar\u2212 versus a wild type strain of Staphylococcus aureus in a rat model of endophthalmitis. Curr. Eye Res.18:177-185.","journal-title":"Curr. Eye Res."},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.63.9.3373-3380.1995"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.3.1304-1311.2000"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2001.02494.x"},{"key":"e_1_3_2_12_2","doi-asserted-by":"crossref","first-page":"2027","DOI":"10.1126\/science.276.5321.2027","volume":"276","year":"1997","unstructured":"Ji, G., R. Beavis, and R. P. Novick. 1997. Bacterial interference caused by autoinducing peptide variants. Science276:2027-2030.","journal-title":"Science"},{"key":"e_1_3_2_13_2","doi-asserted-by":"crossref","first-page":"812","DOI":"10.1093\/infdis\/155.4.812","volume":"155","year":"1987","unstructured":"Kass, E. H., M. I. Kendrick, Y. C. Tsai, and J. Parsonnet. 1987. Interaction of magnesium ion, oxygen tension, and temperature in the production of toxic-shock-syndrome toxin-1 by Staphylococcus aureus. J. Infect. Dis.155:812-815.","journal-title":"J. Infect. Dis."},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/305709a0"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.176.17.5534-5536.1994"},{"key":"e_1_3_2_16_2","first-page":"392","year":"2000","unstructured":"Novick, R. P. 2000. Pathogenicity factors and their regulation, p. 392-407. In V. A. Fischetti, R. P. Novick, J. J. Ferretti, D. A. Portnoy, and J. I. Rood (ed.), Gram-positive pathogens. ASM Press, Washington, D.C.","journal-title":"Gram-positive pathogens"},{"key":"e_1_3_2_17_2","first-page":"360","volume":"69","year":"2001","unstructured":"Orwin, P. M., D. Y. Leung, H. L. Donahue, R. P. Novick, and P. M. Schlievert. 2001. Biochemical and biological properties of staphylococcal enterotoxin K. Infect. Immun.69:360-366.","journal-title":"Biochemical and biological properties of staphylococcal enterotoxin K. Infect. Immun."},{"key":"e_1_3_2_18_2","first-page":"1070","volume":"55","year":"1987","unstructured":"Parsonnet, J., Z. A. Gillis, A. G. Richter, and G. B. Pier. 1987. A rabbit model of toxic shock syndrome that uses a constant, subcutaneous infusion of toxic shock syndrome toxin 1. Infect. Immun.55:1070-1076.","journal-title":"A rabbit model of toxic shock syndrome that uses a constant, subcutaneous infusion of toxic shock syndrome toxin 1. Infect. Immun."},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.170.9.4365-4372.1988"},{"key":"e_1_3_2_20_2","first-page":"55","year":"1997","unstructured":"Projan, S. J., and R. P. Novick. 1997. The molecular basis of pathogenicity, p. 55-81. In K. B. Crossley and G. L. Archer (ed.), The staphylococci in human disease. Churchill Livingstone Inc., New York, N.Y.","journal-title":"The staphylococci in human disease"},{"key":"e_1_3_2_21_2","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1007\/BF00330517","volume":"202","year":"1986","unstructured":"Recsei, P., B. Kreiswirth, M. O'Reilly, P. Schlievert, A. Gruss, and R. P. Novick. 1986. Regulation of exoprotein gene expression in Staphylococcus aureus by agr. Mol. Gen. Genet.202:58-61.","journal-title":"Mol. Gen. Genet."},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.9.5205-5209.2000"},{"key":"e_1_3_2_23_2","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1099\/00222615-24-1-75","volume":"24","year":"1987","unstructured":"Sarafian, S. K., and S. A. Morse. 1987. Environmental factors affecting toxic shock syndrome toxin-1 (TSST-1) synthesis. J. Med. Microbiol.24:75-81.","journal-title":"J. Med. Microbiol."},{"key":"e_1_3_2_24_2","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1093\/infdis\/147.2.236","volume":"147","year":"1983","unstructured":"Schlievert, P. M., and D. A. Blomster. 1983. Production of staphylococcal pyrogenic exotoxin type C: influence of physical and chemical factors. J. Infect. Dis.147:236-242.","journal-title":"J. Infect. Dis."},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.39.1.383-387.1983"},{"key":"e_1_3_2_26_2","first-page":"414","year":"2000","unstructured":"Tenover, F. C., and R. P. Gaynes. 2000. The epidemiology of Staphylococcus aureus infections, p. 414-421. In V. A. Fischetti, R. P. Novick, J. J. Ferretti, D. A. Portnoy, and J. I. Rood (ed.), Gram-positive pathogens. ASM Press, Washington, D.C.","journal-title":"Gram-positive pathogens"},{"key":"e_1_3_2_27_2","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1093\/infdis\/155.4.673","volume":"155","year":"1987","unstructured":"Todd, J. K., B. H. Todd, A. Franco-Buff, C. M. Smith, and D. W. Lawellin. 1987. Influence of focal growth conditions on the pathogenesis of toxic shock syndrome. J. Infect. Dis.155:673-681.","journal-title":"J. Infect. Dis."},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.5.1797-1803.2000"}],"container-title":["Journal of Bacteriology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jb.184.4.1095-1101.2002","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jb.184.4.1095-1101.2002","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T18:07:22Z","timestamp":1627582042000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/jb.184.4.1095-1101.2002"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,2,15]]},"references-count":27,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2002,2,15]]}},"alternative-id":["10.1128\/jb.184.4.1095-1101.2002"],"URL":"https:\/\/doi.org\/10.1128\/jb.184.4.1095-1101.2002","relation":{},"ISSN":["0021-9193","1098-5530"],"issn-type":[{"value":"0021-9193","type":"print"},{"value":"1098-5530","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,2,15]]},"assertion":[{"value":"2001-09-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2001-11-08","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-20","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}