{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T00:01:47Z","timestamp":1791331307138,"version":"4.3.1"},"reference-count":65,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2002,2,15]],"date-time":"2002-02-15T00:00:00Z","timestamp":1013731200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2002,2,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Many surface proteins which are covalently linked to the cell wall of gram-positive bacteria have a consensus C-terminal motif, Leu-Pro-X-Thr-Gly (LPXTG). This sequence is cleaved, and the processed protein is attached to an amino group of a cross-bridge in the peptideglycan by a specific enzyme called sortase. Using the type strain of\n            <jats:italic>Streptococcus suis<\/jats:italic>\n            , NCTC 10234, we found five genes encoding proteins that were homologous to sortases of other bacteria and determined the nucleotide sequences of the genetic regions. One gene, designated\n            <jats:italic>srtA<\/jats:italic>\n            , was linked to\n            <jats:italic>gyrA<\/jats:italic>\n            , as were the sortase and sortase-like genes of other streptococci. Three genes, designated\n            <jats:italic>srtB<\/jats:italic>\n            ,\n            <jats:italic>srtC<\/jats:italic>\n            , and\n            <jats:italic>srtD<\/jats:italic>\n            , were tandemly clustered in a different location, where there were three segments of directly repeated sequences of approximately 110 bp in close vicinity. The remaining gene, designated\n            <jats:italic>srtE<\/jats:italic>\n            , was located separately on the chromosome with a pseudogene which may encode a transposase. The deduced amino acid sequences of the five Srt proteins showed 18 to 31% identity with the sortases of\n            <jats:italic>Streptococcus gordonii<\/jats:italic>\n            and\n            <jats:italic>Staphylococcus aureus<\/jats:italic>\n            , except that SrtA of\n            <jats:italic>S. suis<\/jats:italic>\n            had 65% identity with that of\n            <jats:italic>S. gordonii<\/jats:italic>\n            . Isogenic mutants deficient for\n            <jats:italic>srtA<\/jats:italic>\n            ,\n            <jats:italic>srtBCD<\/jats:italic>\n            , or\n            <jats:italic>srtE<\/jats:italic>\n            were generated by allelic exchanges. The protein fraction which was released from partially purified cell walls by digestion with\n            <jats:italic>N<\/jats:italic>\n            -acetylmuramidase was profiled by two-dimensional gel electrophoresis. More than 15 of the protein spots were missing in the profile of the\n            <jats:italic>srtA<\/jats:italic>\n            mutant compared with that of the parent strain, and this phenotype was completely complemented by\n            <jats:italic>srtA<\/jats:italic>\n            cloned from\n            <jats:italic>S. suis<\/jats:italic>\n            . Four genes encoding proteins corresponding to such spots were identified and sequenced. The deduced translational products of the four genes possessed the LPXTG motif in their C-terminal regions. On the other hand, the protein spots that were missing in the\n            <jats:italic>srtA<\/jats:italic>\n            mutant appeared in the profiles of the\n            <jats:italic>srtBCD<\/jats:italic>\n            and\n            <jats:italic>srtE<\/jats:italic>\n            mutants. These results provide evidence that the cell wall sorting system involving\n            <jats:italic>srtA<\/jats:italic>\n            is also present in\n            <jats:italic>S. suis<\/jats:italic>\n            .\n          <\/jats:p>","DOI":"10.1128\/jb.184.4.971-982.2002","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:55:53Z","timestamp":1027763753000},"page":"971-982","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":91,"title":["Characterization of\n            <i>Streptococcus suis<\/i>\n            Genes Encoding Proteins Homologous to Sortase of Gram-Positive Bacteria"],"prefix":"10.1128","volume":"184","author":[{"given":"Makoto","family":"Osaki","sequence":"first","affiliation":[{"name":"National Institute of Animal Health, Tsukuba, Ibaraki, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daisuke","family":"Takamatsu","sequence":"additional","affiliation":[{"name":"National Institute of Animal Health, Tsukuba, Ibaraki, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshihiro","family":"Shimoji","sequence":"additional","affiliation":[{"name":"National Institute of Animal Health, Tsukuba, Ibaraki, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tsutomu","family":"Sekizaki","sequence":"additional","affiliation":[{"name":"National Institute of Animal Health, Tsukuba, Ibaraki, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"381","year":"1993","unstructured":"Archibald, A. 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