{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T07:15:26Z","timestamp":1792653326048,"version":"4.5.0"},"reference-count":39,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2002,2,15]],"date-time":"2002-02-15T00:00:00Z","timestamp":1013731200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2002,2,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A new member of the IS\n            <jats:italic>605<\/jats:italic>\n            transposable element family, designated IS\n            <jats:italic>Hp608<\/jats:italic>\n            , was found by subtractive hybridization in\n            <jats:italic>Helicobacter pylori<\/jats:italic>\n            . Like the three other insertion sequences (ISs) known in this gastric pathogen, it contains two open reading frames (\n            <jats:italic>orfA<\/jats:italic>\n            and\n            <jats:italic>orfB<\/jats:italic>\n            ), each related to putative transposase genes of simpler (one-gene) elements in other prokaryotes;\n            <jats:italic>orfB<\/jats:italic>\n            is also related to the\n            <jats:italic>Salmonella<\/jats:italic>\n            virulence gene\n            <jats:italic>gipA<\/jats:italic>\n            . PCR and hybridization tests showed that IS\n            <jats:italic>Hp608<\/jats:italic>\n            is nonrandomly distributed geographically: it was found in 21% of 194 European and African strains, 14% of 175 Bengali strains, 43% of 131 strains from native Peruvians and Alaska natives, but just 1% of 223 East Asian strains. IS\n            <jats:italic>Hp608<\/jats:italic>\n            also seemed more abundant in Peruvian gastric cancer strains than gastritis strains (9 of 14 versus 15 of 45, respectively;\n            <jats:italic>P<\/jats:italic>\n            = 0.04). Two IS\n            <jats:italic>Hp608<\/jats:italic>\n            types differing by \u223c11% in DNA sequence were identified: one was widely distributed geographically, and the other was found only in Peruvian and Alaskan strains. Isolates of a given type differed by \u22642% in DNA sequence, but several recombinant elements were also found. IS\n            <jats:italic>Hp608<\/jats:italic>\n            marked with a resistance gene was found to (i) transpose in\n            <jats:italic>Escherichia coli<\/jats:italic>\n            ; (ii) generate simple insertions during transposition, not cointegrates; (iii) insert downstream of the motif 5\"-TTAC without duplicating target sequences; and (iv) require\n            <jats:italic>orfA<\/jats:italic>\n            but not\n            <jats:italic>orfB<\/jats:italic>\n            for its transposition. IS\n            <jats:italic>Hp608<\/jats:italic>\n            represents a widespread family of novel chimeric mobile DNA elements whose further analysis should provide new insights into transposition mechanisms and into microbial population genetic structure and genome evolution.\n          <\/jats:p>","DOI":"10.1128\/jb.184.4.992-1002.2002","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:55:53Z","timestamp":1027763753000},"page":"992-1002","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":81,"title":["Transposable Element IS\n            <i>Hp608<\/i>\n            of\n            <i>Helicobacter pylori<\/i>\n            : Nonrandom Geographic Distribution, Functional Organization, and Insertion Specificity"],"prefix":"10.1128","volume":"184","author":[{"given":"Dangeruta","family":"Kersulyte","sequence":"first","affiliation":[{"name":"Departments of Molecular Microbiology and Genetics, Washington University School of Medicine, St. Louis, Missouri 63110"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Billie","family":"Velapatin\u0303o","sequence":"additional","affiliation":[{"name":"Departments of Molecular Microbiology and Genetics, Washington University School of Medicine, St. Louis, Missouri 63110"},{"name":"Department of Pathology, Universidad Peruana Cayetano Heredia, Lima, Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giedrius","family":"Dailide","sequence":"additional","affiliation":[{"name":"Departments of Molecular Microbiology and Genetics, Washington University School of Medicine, St. Louis, Missouri 63110"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asish K.","family":"Mukhopadhyay","sequence":"additional","affiliation":[{"name":"Departments of Molecular Microbiology and Genetics, Washington University School of Medicine, St. Louis, Missouri 63110"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshiyuki","family":"Ito","sequence":"additional","affiliation":[{"name":"Departments of Molecular Microbiology and Genetics, Washington University School of Medicine, St. Louis, Missouri 63110"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lizbeth","family":"Cahuayme","sequence":"additional","affiliation":[{"name":"Department of Pathology, Universidad Peruana Cayetano Heredia, Lima, Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alan J.","family":"Parkinson","sequence":"additional","affiliation":[{"name":"Arctic Investigations Program, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Anchorage, Alaska"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert H.","family":"Gilman","sequence":"additional","affiliation":[{"name":"Department of Pathology, Universidad Peruana Cayetano Heredia, Lima, Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Douglas E.","family":"Berg","sequence":"additional","affiliation":[{"name":"Departments of Molecular Microbiology and Genetics, Washington University School of Medicine, St. Louis, Missouri 63110"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1046\/j.1365-2958.1999.01382.x","volume":"32","year":"1999","unstructured":"Achtman, M., T. 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