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Recent studies have characterized the circular plasmid regions that confer autonomous replication, but the genetic elements necessary for linear plasmid maintenance have not been experimentally identified. Two vectors derived from linear plasmids lp25 and lp28-1 were constructed and shown to replicate autonomously in<jats:italic>B. burgdorferi<\/jats:italic>. These vectors identify internal regions of linear plasmids necessary for autonomous replication in<jats:italic>B. burgdorferi<\/jats:italic>. Although derived from linear plasmids, the vectors are maintained in circular form in<jats:italic>B. burgdorferi<\/jats:italic>, indicating that plasmid maintenance functions are conserved, regardless of DNA form. Finally, derivatives of these vectors indicate that paralogous gene family 49 is apparently not required for either circular or linear plasmid replication.<\/jats:p>","DOI":"10.1128\/jb.185.10.3202-3209.2003","type":"journal-article","created":{"date-parts":[[2003,5,2]],"date-time":"2003-05-02T16:23:48Z","timestamp":1051892628000},"page":"3202-3209","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":44,"title":["Conservation of Plasmid Maintenance Functions between Linear and Circular Plasmids in<i>Borrelia burgdorferi<\/i>"],"prefix":"10.1128","volume":"185","author":[{"given":"Philip E.","family":"Stewart","sequence":"first","affiliation":[{"name":"Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George","family":"Chaconas","sequence":"additional","affiliation":[{"name":"Department of Biochemistry &amp; Molecular Biology, University of Calgary, Calgary, Alberta T2N 4N1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia","family":"Rosa","sequence":"additional","affiliation":[{"name":"Laboratory of Human Bacterial Pathogenesis, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.7043737"},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1016\/S1369-5274(99)00012-0","volume":"2","year":"1999","unstructured":"Casjens, S. 1999. 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