{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T22:35:43Z","timestamp":1791153343484,"version":"4.1.0"},"reference-count":20,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2003,6,1]],"date-time":"2003-06-01T00:00:00Z","timestamp":1054425600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2003,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The transcription of\n            <jats:italic>mecA<\/jats:italic>\n            , the gene required for oxacillin resistance in staphylococci, was quantified in a collection of 65 geographically and genetically diverse clinical and 8 defined laboratory\n            <jats:italic>Staphylococcus aureus<\/jats:italic>\n            isolates.\n            <jats:italic>mecA<\/jats:italic>\n            transcription was measured by real-time reverse transcription-PCR, confirmed by Northern blot analysis, and correlated with the presence and DNA sequence of the two\n            <jats:italic>mecA<\/jats:italic>\n            repressors,\n            <jats:italic>mecI<\/jats:italic>\n            and\n            <jats:italic>blaI<\/jats:italic>\n            . Isolates were first examined that contained\n            <jats:italic>mecI<\/jats:italic>\n            and\/or\n            <jats:italic>blaI<\/jats:italic>\n            with wild-type sequence. BlaI provided significantly more repression of\n            <jats:italic>mecA<\/jats:italic>\n            transcription than did MecI, unrelated to\n            <jats:italic>blaI<\/jats:italic>\n            genetic location. Both together repressed\n            <jats:italic>mecA<\/jats:italic>\n            better than either one alone. In clinical isolates containing only wild-type\n            <jats:italic>mecI<\/jats:italic>\n            ,\n            <jats:italic>mecA<\/jats:italic>\n            transcription repression was 10- to 25-fold less effective than that seen in previously studied constructs derived from strain N315. There was a difference in the\n            <jats:italic>mecI<\/jats:italic>\n            ribosomal binding site (RBS) between the clinical isolates (GGAA) and N315 (GGAG). The GGAA RBS was associated with 5.5- to 7.3-fold less\n            <jats:italic>mecA<\/jats:italic>\n            repression than GGAG in isogenic constructs. The values generated for wild-type repressors were compared to those in 26 isolates containing\n            <jats:italic>mecI<\/jats:italic>\n            mutations.\n            <jats:italic>mecA<\/jats:italic>\n            transcription appeared to be repressed only by BlaI in isolates with\n            <jats:italic>mecI<\/jats:italic>\n            nonsense and frameshift mutations. In contrast,\n            <jats:italic>mecI<\/jats:italic>\n            repression seemed to be partially or fully retained in many of the isolates with\n            <jats:italic>mecI<\/jats:italic>\n            and one isolate with\n            <jats:italic>blaI<\/jats:italic>\n            missense mutations, providing structure-function correlates with the site and type of mutation. We conclude that\n            <jats:italic>mecA<\/jats:italic>\n            repressor activity is highly variable in clinical\n            <jats:italic>S. aureus<\/jats:italic>\n            isolates due to\n            <jats:italic>mecI<\/jats:italic>\n            mutations, RBS polymorphisms, and unidentified genomic adaptations.\n          <\/jats:p>","DOI":"10.1128\/jb.185.11.3446-3452.2003","type":"journal-article","created":{"date-parts":[[2003,5,16]],"date-time":"2003-05-16T23:10:49Z","timestamp":1053126649000},"page":"3446-3452","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":27,"title":["Quantitation of\n            <i>mecA<\/i>\n            Transcription in Oxacillin-Resistant\n            <i>Staphylococcus aureus<\/i>\n            Clinical Isolates"],"prefix":"10.1128","volume":"185","author":[{"given":"Adriana E.","family":"Rosato","sequence":"first","affiliation":[{"name":"Departments of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William A.","family":"Craig","sequence":"additional","affiliation":[{"name":"Departments of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gordon L.","family":"Archer","sequence":"additional","affiliation":[{"name":"Departments of Medicine"},{"name":"Microbiology\/Immunology, Medical College of Virginia Campus of Virginia Commonwealth University, Richmond, Virginia 23298-0049"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.38.3.447"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.40.4.924"},{"key":"e_1_3_2_4_2","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1172\/JCI111965","volume":"76","year":"1985","unstructured":"Chambers, H. 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The penicillin sensory transducer, BlaR, involved in the inducibility of beta-lactamase synthesis in Bacillus licheniformis is embedded in the plasma membrane via a four-alpha-helix bundle. Mol. Microbiol.23:935-944.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_11_2","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/0014-5793(92)80039-J","volume":"298","year":"1992","unstructured":"Hiramatsu, K., K. Asada, E. Suzuki, K. Okonogi, and T. Yokota. 1992. Molecular cloning and nucleotide sequence determination of the regulator region of mecA gene in methicillin-resistant Staphylococcus aureus (MRSA). FEBS Lett.298:133-136.","journal-title":"FEBS Lett."},{"key":"e_1_3_2_12_2","doi-asserted-by":"crossref","unstructured":"Joris B. K. Hardt and J. M. Ghuysen. 1994. Induction of B-lactamase and low affinity penicillin binding protein 2\u2032 synthesis in gram positive bacteria p. 505-515. In J. M. Ghuysen and R. Hakenbeck (ed.) Bacterial cell wall. 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Science291:1962-1965.","journal-title":"Science"}],"container-title":["Journal of Bacteriology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JB.185.11.3446-3452.2003","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JB.185.11.3446-3452.2003","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T18:08:35Z","timestamp":1627582115000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JB.185.11.3446-3452.2003"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,6]]},"references-count":20,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2003,6]]}},"alternative-id":["10.1128\/JB.185.11.3446-3452.2003"],"URL":"https:\/\/doi.org\/10.1128\/jb.185.11.3446-3452.2003","relation":{},"ISSN":["0021-9193","1098-5530"],"issn-type":[{"value":"0021-9193","type":"print"},{"value":"1098-5530","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,6]]},"assertion":[{"value":"2003-01-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2003-03-12","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-20","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}