{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T05:46:28Z","timestamp":1789364788356,"version":"build-2803163510"},"reference-count":54,"publisher":"American Society for Microbiology","issue":"13","license":[{"start":{"date-parts":[[2003,7,1]],"date-time":"2003-07-01T00:00:00Z","timestamp":1057017600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2003,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Some genes present in only certain strains of the genetically diverse gastric pathogen<jats:italic>Helicobacter pylori<\/jats:italic>may affect its phenotype and\/or evolutionary potential. Here we describe a new 16.3-kb segment, 7 of whose 16 open reading frames are homologs of type IV secretion genes (<jats:italic>virB4<\/jats:italic>,<jats:italic>virB7<\/jats:italic>to<jats:italic>virB11<\/jats:italic>, and<jats:italic>virD4<\/jats:italic>), the third such putative secretion gene cluster found in<jats:italic>H. pylori<\/jats:italic>. This segment, to be called<jats:italic>tfs3<\/jats:italic>, was discovered by subtractive hybridization and chromosome walking. Full-length and truncated<jats:italic>tfs3<\/jats:italic>elements were found in 20 and 19%, respectively, of 94 strains tested, which were from Spain, Peru, India, and Japan. A<jats:italic>tfs3<\/jats:italic>remnant (6 kb) was found in an archived stock of reference strain J99, although it was not included in this strain's published genome sequence. PCR and DNA sequence analyses indicated the following. (i)<jats:italic>tfs3<\/jats:italic>'s ends are conserved. (ii) Right-end insertion occurred at one specific site in a chromosomal region that is varied in gene content and arrangement, the \u201cplasticity zone.\u201d (iii) Left-end insertion occurred at different sites in each of nine strains studied. (iv) Sequences next to the right-end target in<jats:italic>tfs3<\/jats:italic>-free strains were absent from most strains carrying full-length<jats:italic>tfs3<\/jats:italic>elements. These patterns suggested insertion by a transposition-like event, but one in which targets are chosen with little or no specificity at the left end and high specificity at the right end, thereby deleting the intervening DNA.<\/jats:p>","DOI":"10.1128\/jb.185.13.3764-3772.2003","type":"journal-article","created":{"date-parts":[[2003,6,17]],"date-time":"2003-06-17T17:59:39Z","timestamp":1055872779000},"page":"3764-3772","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":74,"title":["Cluster of Type IV Secretion Genes in<i>Helicobacter pylori<\/i>'s Plasticity Zone"],"prefix":"10.1128","volume":"185","author":[{"given":"Dangeruta","family":"Kersulyte","sequence":"first","affiliation":[{"name":"Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri 63110"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Billie","family":"Velapatin\u0303o","sequence":"additional","affiliation":[{"name":"Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri 63110"},{"name":"Department of Microbiology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asish K.","family":"Mukhopadhyay","sequence":"additional","affiliation":[{"name":"Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri 63110"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lizbeth","family":"Cahuayme","sequence":"additional","affiliation":[{"name":"Department of Microbiology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alejandro","family":"Bussalleu","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Facultad de Medecina, Universidad Peruana Cayetano Heredia, Lima 31, Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Combe","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Facultad de Medecina, Universidad Peruana Cayetano Heredia, Lima 31, Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert H.","family":"Gilman","sequence":"additional","affiliation":[{"name":"Department of Microbiology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Douglas E.","family":"Berg","sequence":"additional","affiliation":[{"name":"Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri 63110"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1046\/j.1365-2958.1998.00770.x","volume":"28","year":"1998","unstructured":"Akopyants, N. S., S. W. Clifton, D. Kersulyte, J. E. Crabtree, B. E. Youree, C. A. Reece, N. O. Bukanov, E. S. Drazek, B. A. Roe, and D. E. Berg. 1998. Analyses of the cag pathogenicity island of Helicobacter pylori. Mol. Microbiol.28:37-53.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"13108","DOI":"10.1073\/pnas.95.22.13108","volume":"95","year":"1998","unstructured":"Akopyants, N. S., A. Fradkov, L. Diatchenko, P. D. Siebert, S. Lukyanov, E. D. Sverdlov, and D. E. Berg. 1998. PCR-based subtractive hybridization and differences in gene content among strains of Helicobacter pylori. Proc. Natl. Acad. Sci. USA95:13108-13113.","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"e_1_3_2_4_2","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1038\/16495","volume":"397","year":"1999","unstructured":"Alm, R. A., L. S. Ling, D. T. Moir, B. L. King, E. D. Brown, P. C. Doig, D. R. Smith, B. Noonan, B. C. Guild, B. L. deJonge, G. Carmel, P. J. Tummino, A. Caruso, M. Uria-Nickelsen, D. M. Mills, C. Ives, R. Gibson, D. Merberg, S. D. Mills, Q. Jiang, D. E. Taylor, G. F. Vovis, and T. J. Trust. 1999. Genomic-sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori. Nature397:176-180.","journal-title":"Nature"},{"key":"e_1_3_2_5_2","doi-asserted-by":"crossref","first-page":"677","DOI":"10.1046\/j.1462-5822.2002.00222.x","volume":"4","year":"2002","unstructured":"Amieva, M. R., N. R. Salama, L. S. Tompkins, and S. Falkow. 2002. Helicobacter pylori enter and survive within multivesicular vacuoles of epithelial cells. Cell Microbiol.4:677-690.","journal-title":"Cell Microbiol."},{"key":"e_1_3_2_6_2","doi-asserted-by":"crossref","first-page":"741","DOI":"10.1016\/S0092-8674(03)00197-1","volume":"112","year":"2003","unstructured":"Amundsen, S. K., and G. R. Smith. 2003. Interchangeable parts of the Escherichia coli recombination machinery. Cell112:741-744.","journal-title":"Cell"},{"key":"e_1_3_2_7_2","doi-asserted-by":"crossref","first-page":"173.","DOI":"10.1126\/science.267.5195.173","volume":"267","year":"1995","unstructured":"Anonymous. 1995. The Gold Bug: Helicobacter pylori. Science267:173.","journal-title":"Science"},{"key":"e_1_3_2_8_2","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1006\/jmbi.2000.3861","volume":"300","year":"2000","unstructured":"Arnold, D. A., N. Handa, I. Kobayashi, and S. C. Kowalczykowski. 2000. A novel, 11 nucleotide variant of chi, chi*: one of a class of sequences defining the Escherichia coli recombination hotspot chi. J. Mol. Biol.300:469-479.","journal-title":"J. Mol. Biol."},{"key":"e_1_3_2_9_2","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1006\/jmbi.1999.2758","volume":"289","year":"1999","unstructured":"Ashizawa, Y., T. Yokochi, Y. Ogata, Y. Shobuike, J. Kato, and H. Ikeda. 1999. Mechanism of DNA gyrase-mediated illegitimate recombination: characterization of Escherichia coli gyrA mutations that confer hyper-recombination phenotype. J. Mol. Biol.289:447-458.","journal-title":"J. Mol. Biol."},{"key":"e_1_3_2_10_2","unstructured":"Ausubel F. M. R. Brent R. E. Kingston D. D. Moore J. G. Seidman J. A. Smith and K. A. Struhl. 1994. Current protocols in molecular biology; supplement 27 CPMB p. 2.4.1. Greene Publishing and Wiley Interscience New York N.Y."},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.11.6242-6250.2002"},{"key":"e_1_3_2_12_2","doi-asserted-by":"crossref","first-page":"996","DOI":"10.1086\/516081","volume":"25","year":"1997","unstructured":"Berg, D. E., R. H. Gilman, J. Lelwala-Guruge, K. Srivastava, Y. Valdez, J. Watanabe, J. Miyagi, N. S. Akopyants, A. Ramirez-Ramos, T. H. Yoshiwara, S. Recavarren, and R. Leon-Barua. 1997. Helicobacter pylori populations in individual Peruvian patients. Clin. Infect. Dis.25:996-1002.","journal-title":"Clin. Infect. Dis."},{"key":"e_1_3_2_13_2","doi-asserted-by":"crossref","first-page":"34339","DOI":"10.1074\/jbc.M104256200","volume":"276","year":"2001","unstructured":"Blackwell, L. J., K. P. Bjornson, D. J. Allen, and P. Modrich. 2001. Distinct MutS DNA-binding modes that are differentially modulated by ATP binding and hydrolysis. J. Biol. Chem.276:34339-34347.","journal-title":"J. Biol. Chem."},{"key":"e_1_3_2_14_2","doi-asserted-by":"crossref","first-page":"1047","DOI":"10.1016\/S0300-9084(02)00020-2","volume":"84","year":"2002","unstructured":"Bourniquel, A. A., and T. A. Bickle. 2002. Complex restriction enzymes: NTP-driven molecular motors. Biochimie84:1047-1059.","journal-title":"Biochimie"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-147-12-3201"},{"key":"e_1_3_2_16_2","first-page":"296","volume":"3","year":"1998","unstructured":"Chalkauskas, H., D. Kersulyte, I. Cepuliene, V. Urbonas, D. Ruzeviciene, A. Barakauskiene, A. Raudonikiene, and D. E. Berg. 1998. Genotypes of Helicobacter pylori in Lithuanian families. Helicobacter3:296-302.","journal-title":"Helicobacter"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.4.1259-1268.2001"},{"key":"e_1_3_2_18_2","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1046\/j.1365-2958.2002.02785.x","volume":"43","year":"2002","unstructured":"Chedin, F., and S. C. Kowalczykowski. 2002. A novel family of regulated helicases\/nucleases from gram-positive bacteria: insights into the initiation of DNA recombination. Mol. Microbiol.43:823-834.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_19_2","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1046\/j.1365-2958.2001.02302.x","volume":"40","year":"2001","unstructured":"Christie, P. J. 2001. Type IV secretion: intercellular transfer of macromolecules by systems ancestrally related to conjugation machines. Mol. Microbiol.40:294-305.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_20_2","doi-asserted-by":"crossref","unstructured":"Cover T. L. D. E. Berg M. J. Blaser and H. L. T. Mobley. 2001. H. pylori pathogenesis p. 509-558. In E. A. Groisman (ed.) Principles of bacterial pathogenesis. Academic Press New York N.Y.","DOI":"10.1016\/B978-012304220-0\/50012-1"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.12.3940-3946.2002"},{"key":"e_1_3_2_22_2","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1038\/284601a0","volume":"284","year":"1980","unstructured":"Doolittle, W. F., and C. Sapienza. 1980. Selfish genes, the phenotype paradigm and genome evolution. Nature284:601-603.","journal-title":"Nature"},{"key":"e_1_3_2_23_2","first-page":"646","volume":"88","year":"1980","unstructured":"Dover, G., and W. F. Doolittle. 1980. Modes of genome evolution. Nature88:646-647.","journal-title":"Nature"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.66.4.617-629.2002"},{"key":"e_1_3_2_25_2","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/0378-1119(93)90061-7","volume":"135","year":"1993","unstructured":"Ehrlich, S. D., H. Bierne, E. d'Alencon, D. Vilette, M. Petranovic, P. Noirot, and M. Michel. 1993. Mechanisms of illegitimate recombination. Gene135:161-166.","journal-title":"Gene"},{"key":"e_1_3_2_26_2","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1146\/annurev.micro.54.1.615","volume":"54","year":"2000","unstructured":"Ernst, P. B., and B. D. Gold. 2000. The disease spectrum of Helicobacter pylori: the immunopathogenesis of gastroduodenal ulcer and gastric cancer. Annu. Rev. Microbiol.54:615-640.","journal-title":"Annu. Rev. Microbiol."},{"key":"e_1_3_2_27_2","doi-asserted-by":"crossref","first-page":"15056","DOI":"10.1073\/pnas.251396098","volume":"98","year":"2001","unstructured":"Falush, D., C. Kraft, N. S. Taylor, P. Correa, J. G. Fox, M. Achtman, and S. Suerbaum. 2001. Recombination and mutation during long-term gastric colonization by Helicobacter pylori: estimates of clock rates, recombination size, and minimal age. Proc. Natl. Acad. Sci. USA98:15056-15061.","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"e_1_3_2_28_2","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1078\/1438-4221-00199","volume":"292","year":"2002","unstructured":"Fischer, W., R. Haas, and S. Odenbreit. 2002. Type IV secretion systems in pathogenic bacteria. Int. J. Med. Microbiol.292:159-168.","journal-title":"Int. J. Med. Microbiol."},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2001.02714.x"},{"key":"e_1_3_2_30_2","doi-asserted-by":"crossref","first-page":"12430","DOI":"10.1073\/pnas.96.22.12430","volume":"96","year":"1999","unstructured":"Garcia, L. R., and I. J. Molineux. 1999. Translocation and specific cleavage of bacteriophage T7 DNA in vivo by EcoKI. Proc. Natl. Acad. Sci. USA96:12430-12435.","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"e_1_3_2_31_2","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1046\/j.1365-2958.2001.02502.x","volume":"41","year":"2001","unstructured":"Hofreuter, D., S. Odenbreit, and R. Haas. 2001. Natural transformation competence in Helicobacter pylori is mediated by the basic components of a type IV secretion system. Mol. Microbiol.41:379-391.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_32_2","doi-asserted-by":"crossref","first-page":"14625","DOI":"10.1073\/pnas.251551698","volume":"98","year":"2001","unstructured":"Israel, D. A., N. Salama, U. Krishna, U. M. Rieger, J. C. Atherton, S. Falkow, and R. M. Peek, Jr. 2001. Helicobacter pylori genetic diversity within the gastric niche of a single human host. Proc. Natl. Acad. Sci. USA98:14625-14630.","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.175.5.1264-1271.1993"},{"key":"e_1_3_2_34_2","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1006\/jmbi.2000.3729","volume":"299","year":"2000","unstructured":"Juhala, R. J., M. E. Ford, R. L. Duda, A. Youlton, G. F. Hatfull, and R. W. Hendrix. 2000. Genomic sequences of bacteriophages HK97 and HK022: pervasive genetic mosaicism in the lambdoid bacteriophages. J. Mol. Biol.299:27-51.","journal-title":"J. Mol. Biol."},{"key":"e_1_3_2_35_2","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1046\/j.1365-2958.1999.01140.x","volume":"31","year":"1999","unstructured":"Kersulyte, D., H. Chalkauskas., and D. E. Berg. 1999. Emergence of recombinant strains of Helicobacter pylori during human infection. Mol. Microbiol.31:31-43.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.184.4.992-1002.2002"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.180.11.2901-2905.1998"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.4.2108-2120.2002"},{"key":"e_1_3_2_39_2","doi-asserted-by":"crossref","first-page":"1725","DOI":"10.1101\/gad.896801","volume":"15","year":"2001","unstructured":"Lee, V. T., and O. Schneewind. 2001. Protein secretion and the pathogenesis of bacterial infections. Genes Dev.15:1725-1752.","journal-title":"Genes Dev."},{"key":"e_1_3_2_40_2","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1126\/science.1069076","volume":"297","year":"2002","unstructured":"Mahdavi, J., B. Sonden, M. Hurtig, F. O. Olfat, L. Forsberg, N. Roche, J. Angstrom, T. Larsson, S. Teneberg, K. A. Karlsson, S. Altraja, T. Wadstrom, D. Kersulyte, D. E. Berg, A. Dubois, C. Petersson, K. E. Magnusson, T. Norberg, F. Lindh, B. B. Lundskog, A. Arnqvist, L. Hammarstrom, and T. Boren. 2002. Helicobacter pylori SabA adhesin in persistent infection and chronic inflammation. Science297:573-578.","journal-title":"Science"},{"key":"e_1_3_2_41_2","doi-asserted-by":"crossref","unstructured":"Mobley H. L. T. G. L. Mendez and S. L. Hazell (ed.). 2001. Helicobacter pylori : physiology and genetics. ASM Press Washington D.C.","DOI":"10.1128\/9781555818005"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.11.3219-3227.2000"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.64.2.412-434.2000"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.11.6240-6249.2000"},{"key":"e_1_3_2_45_2","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1016\/S0966-842X(02)00038-0","volume":"11","year":"2003","unstructured":"Rhen, M., S. Eriksson, M. Clements, S. Bergstr\u00f6m, and S. J. Normark. 2003. The basis of persistent bacterial infections. Trends Microbiol.11:80-86.","journal-title":"Trends Microbiol."},{"key":"e_1_3_2_46_2","doi-asserted-by":"crossref","first-page":"14668","DOI":"10.1073\/pnas.97.26.14668","volume":"97","year":"2000","unstructured":"Salama, N., K. Guillemin, T. K. McDaniel, G. Sherlock, L. Tompkins, and S. Falkow. 2000. A whole-genome microarray reveals genetic diversity among Helicobacter pylori strains. Proc. Natl. Acad. Sci. USA97:14668-14673.","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"e_1_3_2_47_2","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1034\/j.1600-0854.2002.030303.x","volume":"3","year":"2002","unstructured":"Sexton, J. A., and J. P. Vogel. 2002. Type IVB secretion by intracellular pathogens. Traffic3:178-185.","journal-title":"Traffic"},{"key":"e_1_3_2_48_2","doi-asserted-by":"crossref","first-page":"4677","DOI":"10.1073\/pnas.85.13.4677","volume":"85","year":"1988","unstructured":"Studier, F. W., and P. K. Bandyopadhyay. 1988. Model for how type I restriction enzymes select cleavage sites in DNA. Proc. Natl. Acad. Sci. USA85:4677-4681.","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1038\/41483"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.172.11.6348-6354.1990"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.184.8.2131-2140.2002"},{"key":"e_1_3_2_52_2","doi-asserted-by":"crossref","first-page":"3583","DOI":"10.1002\/j.1460-2075.1985.tb04120.x","volume":"4","year":"1985","unstructured":"Trieu-Cuot, P., G. Gerbaud, T. Lambert, and P. Courvalin. 1985. In vivo transfer of genetic information between gram-positive and gram-negative bacteria. EMBO J.4:3583-3587.","journal-title":"EMBO J."},{"key":"e_1_3_2_53_2","doi-asserted-by":"crossref","first-page":"5410","DOI":"10.1002\/j.1460-2075.1995.tb00225.x","volume":"14","year":"1995","unstructured":"Turlan, C., and M. Chandler. 1995. IS1-mediated intramolecular rearrangements: formation of excised transposon circles and replicative deletions. EMBO J.14:5410-5421.","journal-title":"EMBO J."},{"key":"e_1_3_2_54_2","doi-asserted-by":"crossref","first-page":"638","DOI":"10.1053\/gast.1996.v111.pm8780568","volume":"111","year":"1996","unstructured":"van der Ende, A., E. A. Rauws, M. Feller, C. J. Mulder, G. N. Tytgat, and J. Dankert. 1996. Heterogeneous Helicobacter pylori isolates from members of a family with a history of peptic ulcer disease. Gastroenterology111:638-647.","journal-title":"Gastroenterology"},{"key":"e_1_3_2_55_2","doi-asserted-by":"crossref","first-page":"7874","DOI":"10.1073\/pnas.90.16.7874","volume":"90","year":"1993","unstructured":"Wang, G., R. W. Blakesley, D. E. Berg, and C. M. Berg. 1993. pDUAL: a transposon-based cosmid cloning vector for generating nested deletions and DNA sequencing templates in vivo. Proc. Natl. Acad. Sci. USA90:7874-7878.","journal-title":"Proc. Natl. Acad. Sci. USA"}],"container-title":["Journal of Bacteriology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JB.185.13.3764-3772.2003","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JB.185.13.3764-3772.2003","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,10]],"date-time":"2024-01-10T00:25:49Z","timestamp":1704846349000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JB.185.13.3764-3772.2003"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,7]]},"references-count":54,"journal-issue":{"issue":"13","published-print":{"date-parts":[[2003,7]]}},"alternative-id":["10.1128\/JB.185.13.3764-3772.2003"],"URL":"https:\/\/doi.org\/10.1128\/jb.185.13.3764-3772.2003","relation":{},"ISSN":["0021-9193","1098-5530"],"issn-type":[{"value":"0021-9193","type":"print"},{"value":"1098-5530","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,7]]},"assertion":[{"value":"2003-02-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2003-04-21","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-20","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}