{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T00:06:02Z","timestamp":1793232362027,"version":"4.5.0"},"reference-count":23,"publisher":"American Society for Microbiology","issue":"18","license":[{"start":{"date-parts":[[2003,9,15]],"date-time":"2003-09-15T00:00:00Z","timestamp":1063584000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2003,9,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    We found that\n                    <jats:italic>Lactobacillus reuteri<\/jats:italic>\n                    CRL1098, a lactic acid bacterium isolated from sourdough, is able to produce cobalamin. The sugar-glycerol cofermentation in vitamin B\n                    <jats:sub>12<\/jats:sub>\n                    -free medium showed that this strain was able to reduce glycerol through a well-known cobalamin-dependent reaction with the formation of 1,3-propanediol as a final product. The cell extract of\n                    <jats:italic>L. reuteri<\/jats:italic>\n                    corrected the coenzyme B\n                    <jats:sub>12<\/jats:sub>\n                    requirement of\n                    <jats:italic>Lactobacillus delbrueckii<\/jats:italic>\n                    subsp.\n                    <jats:italic>lactis<\/jats:italic>\n                    ATCC 7830 and allowed the growth of\n                    <jats:italic>Salmonella enterica<\/jats:italic>\n                    serovar Typhimurium (\n                    <jats:italic>metE cbiB<\/jats:italic>\n                    ) and\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    (\n                    <jats:italic>metE<\/jats:italic>\n                    ) in minimal medium. Preliminary genetic studies of cobalamin biosynthesis genes from\n                    <jats:italic>L. reuteri<\/jats:italic>\n                    allowed the identification of\n                    <jats:italic>cob<\/jats:italic>\n                    genes which encode the CobA, CbiJ, and CbiK enzymes involved in the cobalamin pathway. The cobamide produced by\n                    <jats:italic>L. reuteri<\/jats:italic>\n                    , isolated in its cyanide form by using reverse-phase high-pressure liquid chromatography, showed a UV-visible spectrum identical to that of standard cyanocobalamin (vitamin B\n                    <jats:sub>12<\/jats:sub>\n                    ).\n                  <\/jats:p>","DOI":"10.1128\/jb.185.18.5643-5647.2003","type":"journal-article","created":{"date-parts":[[2003,8,29]],"date-time":"2003-08-29T13:45:12Z","timestamp":1062164712000},"page":"5643-5647","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":190,"title":["<i>Lactobacillus reuteri<\/i>\n                    CRL1098 Produces Cobalamin"],"prefix":"10.1128","volume":"185","author":[{"given":"Mari\u0301a P.","family":"Taranto","sequence":"first","affiliation":[{"name":"Centro de Referencia para Lactobacilos (CERELA-CONICET)"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose\u0301 L.","family":"Vera","sequence":"additional","affiliation":[{"name":"Centro de Referencia para Lactobacilos (CERELA-CONICET)"},{"name":"NIZO Food Research and Wageningen Centre for Food Sciences (WCFS), Ede, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeroen","family":"Hugenholtz","sequence":"additional","affiliation":[{"name":"NIZO Food Research and Wageningen Centre for Food Sciences (WCFS), Ede, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Graciela F.","family":"De Valdez","sequence":"additional","affiliation":[{"name":"Centro de Referencia para Lactobacilos (CERELA-CONICET)"},{"name":"Instituto de Microbiologi\u0301a, Facultad de Bioqui\u0301mica, Qui\u0301mica y Farmacia, Universidad Nacional de Tucuma\u0301n, Tucuma\u0301n, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando","family":"Sesma","sequence":"additional","affiliation":[{"name":"Centro de Referencia para Lactobacilos (CERELA-CONICET)"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"781","DOI":"10.1038\/283781a0","volume":"283","year":"1980","unstructured":"Albert, M. 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