{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T00:57:17Z","timestamp":1789606637268,"version":"build-2803163510"},"reference-count":29,"publisher":"American Society for Microbiology","issue":"19","license":[{"start":{"date-parts":[[2003,10,1]],"date-time":"2003-10-01T00:00:00Z","timestamp":1064966400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2003,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            In\n            <jats:italic>Ralstonia<\/jats:italic>\n            sp. strain U2, the\n            <jats:italic>nag<\/jats:italic>\n            catabolic genes, which encode the enzymes for the pathway that catabolizes naphthalene via the alternative ring cleavage gentisate pathway, are transcribed as an operon under the same promoter.\n            <jats:italic>nagR<\/jats:italic>\n            , which encodes a LysR-type transcriptional regulator, is divergently transcribed compared to the\n            <jats:italic>nag<\/jats:italic>\n            catabolic genes. A 4-bp frameshift deletion in\n            <jats:italic>nagR<\/jats:italic>\n            demonstrated that NagR is required for expression of the\n            <jats:italic>nag<\/jats:italic>\n            operon. The transcriptional start of the\n            <jats:italic>nag<\/jats:italic>\n            operon was mapped, and a putative \u221210, \u221235 \u03c3\n            <jats:sup>70<\/jats:sup>\n            -type promoter binding site was identified. Further upstream, a site proximal to the promoter was identified as a site that has bases which have been found to be conserved in the activator-binding motif of other naphthalene pathways. Transcriptional fusion studies demonstrated that NagR regulates the expression of the\n            <jats:italic>nag<\/jats:italic>\n            operon positively in the presence of salicylate and to a lesser extent in the presence of 2-nitrobenzoate. Mutation of the LysR-type activator-binding motif in the\n            <jats:italic>nag<\/jats:italic>\n            promoter-proximal region resulted in a loss of inducibility of a\n            <jats:italic>lacZ<\/jats:italic>\n            reporter gene transcriptionally fused to\n            <jats:italic>nagAa<\/jats:italic>\n            , the first gene of the operon. However, other mutations in the region increased the effectiveness of salicylate as an inducer.\n          <\/jats:p>","DOI":"10.1128\/jb.185.19.5847-5853.2003","type":"journal-article","created":{"date-parts":[[2003,9,16]],"date-time":"2003-09-16T19:17:43Z","timestamp":1063739863000},"page":"5847-5853","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":58,"title":["The Naphthalene Catabolic (\n            <i>nag<\/i>\n            ) Genes of\n            <i>Ralstonia<\/i>\n            sp. Strain U2 Are an Operon That Is Regulated by NagR, a LysR-Type Transcriptional Regulator"],"prefix":"10.1128","volume":"185","author":[{"given":"Rheinallt M.","family":"Jones","sequence":"first","affiliation":[{"name":"School of Biological Sciences, University of Wales Bangor, Bangor, Gwynedd LL57 2UW, Wales, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bethan","family":"Britt-Compton","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, University of Wales Bangor, Bangor, Gwynedd LL57 2UW, Wales, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter A.","family":"Williams","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, University of Wales Bangor, Bangor, Gwynedd LL57 2UW, Wales, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1099\/00221287-88-1-193","volume":"88","year":"1975","unstructured":"Barnsley, E. 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