{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T01:15:32Z","timestamp":1792631732703,"version":"4.5.0"},"reference-count":30,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2003,1,15]],"date-time":"2003-01-15T00:00:00Z","timestamp":1042588800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2003,1,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The \u03b2-lactam resistance gene\n            <jats:italic>mecA<\/jats:italic>\n            was acquired by\n            <jats:italic>Staphylococcus aureus<\/jats:italic>\n            from an extraspecies source. The search for the possible origin of this gene has led to the identification of a close structural homologue of\n            <jats:italic>mecA<\/jats:italic>\n            as a native gene in the animal species\n            <jats:italic>Staphylococcus sciuri.<\/jats:italic>\n            Surprisingly, the overwhelming majority of\n            <jats:italic>S. sciuri<\/jats:italic>\n            isolates were fully susceptible to \u03b2-lactam antibiotics in spite of the ubiquitous presence of the\n            <jats:italic>mecA<\/jats:italic>\n            homologue in the bacteria. We now describe two unusual\n            <jats:italic>S. sciuri<\/jats:italic>\n            strains isolated from humans\u2014SS-37 and SS-41\u2014that showed resistance to methicillin associated with high rates of transcription of the\n            <jats:italic>mecA<\/jats:italic>\n            homologue and production of a protein resembling penicillin binding protein 2a, the gene product of\n            <jats:italic>S. aureus mecA<\/jats:italic>\n            . In strain SS-37 increased transcription of the\n            <jats:italic>mecA<\/jats:italic>\n            homologue was related to insertion of an IS\n            <jats:italic>256<\/jats:italic>\n            element upstream of the structural gene, and strain SS-41 had single nucleotide alterations in the promoter region of the\n            <jats:italic>mecA<\/jats:italic>\n            homologue which appear to be related to up-regulation of the rate of transcription. A third methicillin-resistant human isolate of\n            <jats:italic>S. sciuri<\/jats:italic>\n            that carries both the native\n            <jats:italic>mecA<\/jats:italic>\n            homologue and a methicillin-resistant\n            <jats:italic>S. aureus<\/jats:italic>\n            (MRSA) type\n            <jats:italic>mecA<\/jats:italic>\n            , strain K3, was now shown to be unstable in the absence of drug selection, causing the segregation of antibiotic-susceptible cells accompanied by the loss of the MRSA type\n            <jats:italic>mecA<\/jats:italic>\n            . These observations illustrate the remarkable variety of strategies available to bacteria for acquiring mechanisms of drug resistance in the in vivo environment.\n          <\/jats:p>","DOI":"10.1128\/jb.185.2.645-653.2003","type":"journal-article","created":{"date-parts":[[2003,1,2]],"date-time":"2003-01-02T19:30:24Z","timestamp":1041535824000},"page":"645-653","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":81,"title":["Development of Methicillin Resistance in Clinical Isolates of\n            <i>Staphylococcus sciuri<\/i>\n            by Transcriptional Activation of the\n            <i>mecA<\/i>\n            Homologue Native to the Species"],"prefix":"10.1128","volume":"185","author":[{"given":"Isabel","family":"Couto","sequence":"first","affiliation":[{"name":"Molecular Genetics Unit, Instituto de Tecnologia Qui\u0301mica e Biolo\u0301gica da Universidade Nova de Lisboa, 2780-156 Oeiras, Portugal"},{"name":"Laboratory of Microbiology, The Rockefeller University, New York, New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shang Wei","family":"Wu","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology, The Rockefeller University, New York, New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Tomasz","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology, The Rockefeller University, New York, New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hermi\u0301nia","family":"de Lencastre","sequence":"additional","affiliation":[{"name":"Molecular Genetics Unit, Instituto de Tecnologia Qui\u0301mica e Biolo\u0301gica da Universidade Nova de Lisboa, 2780-156 Oeiras, Portugal"},{"name":"Laboratory of Microbiology, The Rockefeller University, New York, New York"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1016\/0966-842X(94)90608-4","volume":"2","year":"1994","unstructured":"Archer, G. 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