{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T12:20:31Z","timestamp":1790943631601,"version":"4.1.0"},"reference-count":35,"publisher":"American Society for Microbiology","issue":"20","license":[{"start":{"date-parts":[[2003,10,15]],"date-time":"2003-10-15T00:00:00Z","timestamp":1066176000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2003,10,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Large-scale genomic rearrangements including inversions, deletions, and duplications are significant in bacterial evolution. The recently completed\n            <jats:italic>Brucella melitensis<\/jats:italic>\n            16M and\n            <jats:italic>Brucella suis<\/jats:italic>\n            1330 genomes have facilitated the investigation of such events in the\n            <jats:italic>Brucella<\/jats:italic>\n            spp. Suppressive subtractive hybridization (SSH) was employed in identifying genomic differences between\n            <jats:italic>B. melitensis<\/jats:italic>\n            16M and\n            <jats:italic>Brucella abortus<\/jats:italic>\n            2308. Analysis of 45 SSH clones revealed several deletions on chromosomes of\n            <jats:italic>B. abortus<\/jats:italic>\n            and\n            <jats:italic>B. melitensis<\/jats:italic>\n            that encoded proteins of various metabolic pathways. A 640-kb inversion on chromosome II of\n            <jats:italic>B. abortus<\/jats:italic>\n            has been reported previously (S. Michaux Charachon, G. Bourg, E. Jumas Bilak, P. Guigue Talet, A. Allardet Servent, D. O'Callaghan, and M. Ramuz, J. Bacteriol.\n            <jats:bold>179:<\/jats:bold>\n            3244-3249, 1997) and is further described in this study. One end of the inverted region is located on a deleted TATGC site between open reading frames BMEII0292 and BMEII0293. The other end inserted at a GTGTC site of the cyclic-di-GMP phosphodiesterase A (PDEA) gene (BMEII1009), dividing PDEA into two unequal DNA segments of 160 and 977 bp. As a consequence of inversion, the 160-bp segment that encodes the N-terminal region of PDEA was relocated at the opposite end of the inverted chromosomal region. The splitting of the PDEA gene most likely inactivated the function of this enzyme. A recombination mechanism responsible for this inversion is proposed.\n          <\/jats:p>","DOI":"10.1128\/jb.185.20.6130-6136.2003","type":"journal-article","created":{"date-parts":[[2003,10,2]],"date-time":"2003-10-02T22:52:58Z","timestamp":1065135178000},"page":"6130-6136","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":14,"title":["Molecular Characterization of\n            <i>Brucella abortus<\/i>\n            Chromosome II Recombination"],"prefix":"10.1128","volume":"185","author":[{"given":"Georgios","family":"Tsoktouridis","sequence":"first","affiliation":[{"name":"Institute of Molecular Biology and Medicine, The University of Scranton, Scranton, Pennsylvania 18510"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian A.","family":"Merz","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biology and Medicine, The University of Scranton, Scranton, Pennsylvania 18510"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon P.","family":"Manning","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biology and Medicine, The University of Scranton, Scranton, Pennsylvania 18510"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rene\u0301e","family":"Giovagnoli-Kurtz","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biology and Medicine, The University of Scranton, Scranton, Pennsylvania 18510"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leanne E.","family":"Williams","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biology and Medicine, The University of Scranton, Scranton, Pennsylvania 18510"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cesar V.","family":"Mujer","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biology and Medicine, The University of Scranton, Scranton, Pennsylvania 18510"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sue","family":"Hagius","sequence":"additional","affiliation":[{"name":"Department of Veterinary Science, Louisiana State University AgCenter, Baton Rouge, Louisiana 70803"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip","family":"Elzer","sequence":"additional","affiliation":[{"name":"Department of Veterinary Science, Louisiana State University AgCenter, Baton Rouge, Louisiana 70803"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rajendra J.","family":"Redkar","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biology and Medicine, The University of Scranton, Scranton, Pennsylvania 18510"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guy","family":"Patra","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biology and Medicine, The University of Scranton, Scranton, Pennsylvania 18510"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vito G.","family":"DelVecchio","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biology and Medicine, The University of Scranton, Scranton, Pennsylvania 18510"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"81","volume":"20","year":"1998","unstructured":"Bercovich, Z. 1998. 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