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Using the previously described pBSV2 as a backbone, a shuttle vector, termed pKFSS1, which carries the<jats:italic>aadA<\/jats:italic>open reading frame fused to the<jats:italic>B<\/jats:italic>.<jats:italic>burgdorferi flgB<\/jats:italic>promoter was constructed. The hybrid<jats:italic>flgB<\/jats:italic>promoter-<jats:italic>aadA<\/jats:italic>cassette confers resistance to spectinomycin and streptomycin in both<jats:italic>B<\/jats:italic>.<jats:italic>burgdorferi<\/jats:italic>and<jats:italic>Escherichia coli<\/jats:italic>. pKFSS1 has a replication origin derived from the 9-kb circular plasmid and can be comaintained in<jats:italic>B<\/jats:italic>.<jats:italic>burgdorferi<\/jats:italic>with extant shuttle vector pCE320, which has a replication origin derived from a 32-kb circular plasmid, or pBSV2, despite the fact that pKFSS1 and pBSV2 have the same replication origin. Our results demonstrate the availability of a new selectable marker and shuttle vector for genetically dissecting<jats:italic>B<\/jats:italic>.<jats:italic>burgdorferi<\/jats:italic>at the molecular level.<\/jats:p>","DOI":"10.1128\/jb.185.22.6723-6727.2003","type":"journal-article","created":{"date-parts":[[2003,11,2]],"date-time":"2003-11-02T18:12:22Z","timestamp":1067796742000},"page":"6723-6727","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":160,"title":["<i>aadA<\/i>Confers Streptomycin Resistance in<i>Borrelia burgdorferi<\/i>"],"prefix":"10.1128","volume":"185","author":[{"given":"Kristi L.","family":"Frank","sequence":"first","affiliation":[{"name":"Division of Biological Sciences, The University of Montana, Missoula, Montana 59812"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sharyl F.","family":"Bundle","sequence":"additional","affiliation":[{"name":"Division of Biological Sciences, The University of Montana, Missoula, Montana 59812"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michele E.","family":"Kresge","sequence":"additional","affiliation":[{"name":"Division of Biological Sciences, The University of Montana, Missoula, Montana 59812"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian H.","family":"Eggers","sequence":"additional","affiliation":[{"name":"Center for Microbial Pathogenesis, University of Connecticut Health Center, Farmington, Connecticut 06030"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. 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