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Data obtained from the\ncomparative chemical and structural analysis of LPS samples obtained\nfrom the wild type, the mutant strain, and the complemented mutant\ndemonstrated that the\n            <jats:italic>wabG<\/jats:italic>\n            gene is involved in attachment to\u03b1\n-\n            <jats:sc>l<\/jats:sc>\n            -glycero-\n            <jats:sc>d<\/jats:sc>\n            -manno-heptopyranose II\n(\n            <jats:sc>l<\/jats:sc>\n            ,\n            <jats:sc>d<\/jats:sc>\n            -Hep\n            <jats:italic>p<\/jats:italic>\n            II) at the O-3 position of an\u03b1\n-\n            <jats:sc>d<\/jats:sc>\n            -galactopyranosyluronic acid\n(\u03b1-\n            <jats:sc>d<\/jats:sc>\n            -GalA\n            <jats:italic>p<\/jats:italic>\n            ) residue.\n            <jats:italic>K. pneumoniae<\/jats:italic>\n            nonpolar\n            <jats:italic>wabG<\/jats:italic>\n            mutants were devoid of the cell-attached\ncapsular polysaccharide but were still able to produce capsular\npolysaccharide. Similar results were obtained with\n            <jats:italic>K.\npneumoniae<\/jats:italic>\n            nonpolar\n            <jats:italic>waaC<\/jats:italic>\n            and\n            <jats:italic>waaF<\/jats:italic>\n            mutants, which\nproduce shorter LPS core molecules than do\n            <jats:italic>wabG<\/jats:italic>\n            mutants. Other\nouter core\n            <jats:italic>K. pneumoniae<\/jats:italic>\n            nonpolar mutants in the\n            <jats:italic>waa<\/jats:italic>\n            gene cluster were encapsulated.\n            <jats:italic>K. pneumoniae waaC<\/jats:italic>\n            ,\n            <jats:italic>waaF<\/jats:italic>\n            , and\n            <jats:italic>wabG<\/jats:italic>\n            mutants were avirulent when tested in\ndifferent animal models. Furthermore, these mutants were more sensitive\nto some hydrophobic compounds than the wild-type strains. All these\ncharacteristics were rescued by reintroduction of the\n            <jats:italic>waaC<\/jats:italic>\n            ,\n            <jats:italic>waaF<\/jats:italic>\n            , and\n            <jats:italic>wabG<\/jats:italic>\n            genes from\n            <jats:italic>K.\npneumoniae<\/jats:italic>\n            .\n          <\/jats:p>","DOI":"10.1128\/jb.185.24.7213-7221.2003","type":"journal-article","created":{"date-parts":[[2003,12,2]],"date-time":"2003-12-02T01:43:08Z","timestamp":1070329388000},"page":"7213-7221","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":91,"title":["The\n            <i>Klebsiella pneumoniae wabG<\/i>\n            Gene: Role inBiosynthesis of the Core Lipopolysaccharide andVirulence"],"prefix":"10.1128","volume":"185","author":[{"given":"Luis","family":"Izquierdo","sequence":"first","affiliation":[{"name":"Departamento de\rMicrobiologi\u0301a, Facultad de Biologi\u0301a,Universidad de Barcelona, Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nu\u0301ria","family":"Coderch","sequence":"additional","affiliation":[{"name":"Departamento\rde Microbiologi\u0301a y Parasitologi\u0301a Sanitarias,\rDivisio\u0301n de Ciencias de la Salud, Facultad de\rFarmacia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nuria","family":"Pique\u0301","sequence":"additional","affiliation":[{"name":"Departamento\rde Microbiologi\u0301a y Parasitologi\u0301a Sanitarias,\rDivisio\u0301n de Ciencias de la Salud, Facultad de\rFarmacia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emiliano","family":"Bedini","sequence":"additional","affiliation":[{"name":"Dipartimento di\rChimica e Biochimica, Universita\u0300 Federico II di Napoli, Naples,\rItaly"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Michela","family":"Corsaro","sequence":"additional","affiliation":[{"name":"Dipartimento di\rChimica e Biochimica, Universita\u0300 Federico II di Napoli, Naples,\rItaly"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susana","family":"Merino","sequence":"additional","affiliation":[{"name":"Departamento de\rMicrobiologi\u0301a, Facultad de Biologi\u0301a,Universidad de Barcelona, Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra","family":"Fresno","sequence":"additional","affiliation":[{"name":"Departamento de\rMicrobiologi\u0301a, Facultad de Biologi\u0301a,Universidad de Barcelona, Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan M.","family":"Toma\u0301s","sequence":"additional","affiliation":[{"name":"Departamento de\rMicrobiologi\u0301a, Facultad de Biologi\u0301a,Universidad de Barcelona, Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miguel","family":"Regue\u0301","sequence":"additional","affiliation":[{"name":"Departamento\rde Microbiologi\u0301a y Parasitologi\u0301a Sanitarias,\rDivisio\u0301n de Ciencias de la Salud, Facultad de\rFarmacia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1111\/j.1574-6968.1993.tb06354.x","volume":"111","year":"1993","unstructured":"Camprub\u00ed, S., S. 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