{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T19:42:39Z","timestamp":1773085359628,"version":"3.50.1"},"reference-count":29,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2003,3,1]],"date-time":"2003-03-01T00:00:00Z","timestamp":1046476800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2003,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A recombinant clone encoding enzymes for\n            <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n            O12-antigen lipopolysaccharide (LPS) was found when we screened for serum resistance of a cosmid-based genomic library of\n            <jats:italic>K. pneumoniae<\/jats:italic>\n            KT776 (O12:K80) introduced into\n            <jats:italic>Escherichia coli<\/jats:italic>\n            DH5\u03b1. A total of eight open reading frames (ORFs) (\n            <jats:italic>wb<\/jats:italic>\n            <jats:sub>O12<\/jats:sub>\n            gene cluster) were necessary to produce\n            <jats:italic>K. pneumoniae<\/jats:italic>\n            O12-antigen LPS in\n            <jats:italic>E. coli<\/jats:italic>\n            K-12. A complete analysis of the\n            <jats:italic>K. pneumoniae wb<\/jats:italic>\n            <jats:sub>O12<\/jats:sub>\n            cluster revealed an interesting coincidence with the\n            <jats:italic>wb<\/jats:italic>\n            <jats:sub>O4<\/jats:sub>\n            cluster of\n            <jats:italic>Serratia marcescens<\/jats:italic>\n            from ORF5 to ORF8 (or WbbL to WbbA). This prompted us to generate mutants of\n            <jats:italic>K. pneumoniae<\/jats:italic>\n            strain KT776 (O12) and to study complementation between the two enterobacterial\n            <jats:italic>wb<\/jats:italic>\n            clusters using mutants of\n            <jats:italic>S. marcescens<\/jats:italic>\n            N28b (O4) obtained previously. Both\n            <jats:italic>wb<\/jats:italic>\n            gene clusters are examples of ABC 2 transporter-dependent pathways for O-antigen heteropolysaccharides. The\n            <jats:italic>wzm<\/jats:italic>\n            -\n            <jats:italic>wzt<\/jats:italic>\n            genes and the\n            <jats:italic>wbbA<\/jats:italic>\n            or\n            <jats:italic>wbbB<\/jats:italic>\n            genes were not interchangeable between the two gene clusters despite their high level of similarity. However, introduction of three cognate genes (\n            <jats:italic>wzm<\/jats:italic>\n            -\n            <jats:italic>wzt<\/jats:italic>\n            -\n            <jats:italic>wbbA<\/jats:italic>\n            or\n            <jats:italic>wzm<\/jats:italic>\n            -\n            <jats:italic>wzt<\/jats:italic>\n            -\n            <jats:italic>wbbB<\/jats:italic>\n            ) into mutants unable to produce O antigen allowed production of the specific O antigen. The\n            <jats:italic>K. pneumoniae<\/jats:italic>\n            O12 WbbL protein performs the same function as WbbL from\n            <jats:italic>S. marcescens<\/jats:italic>\n            O4 in either the\n            <jats:italic>S. marcescens<\/jats:italic>\n            O4 or\n            <jats:italic>E. coli<\/jats:italic>\n            K-12 genetic background.\n          <\/jats:p>","DOI":"10.1128\/jb.185.5.1634-1641.2003","type":"journal-article","created":{"date-parts":[[2003,2,18]],"date-time":"2003-02-18T23:46:40Z","timestamp":1045612000000},"page":"1634-1641","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":27,"title":["Synthesis of a\n            <i>Klebsiella pneumoniae<\/i>\n            O-Antigen Heteropolysaccharide (O12) Requires an ABC 2 Transporter"],"prefix":"10.1128","volume":"185","author":[{"given":"Luis","family":"Izquierdo","sequence":"first","affiliation":[{"name":"Departamento de Microbiologi\u0301a, Facultad de Biologi\u0301a, Universidad de Barcelona, 08071 Barcelona"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susana","family":"Merino","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologi\u0301a, Facultad de Biologi\u0301a, Universidad de Barcelona, 08071 Barcelona"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miguel","family":"Regue\u0301","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologi\u0301a y Parasitologi\u0301a Sanitarias, Divisio\u0301n de Ciencias de la Salud, Facultad de Farmacia, Universidad de Barcelona, 08028 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Florencia","family":"Rodriguez","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologi\u0301a y Parasitologi\u0301a Sanitarias, Divisio\u0301n de Ciencias de la Salud, Facultad de Farmacia, Universidad de Barcelona, 08028 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan M.","family":"Toma\u0301s","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologi\u0301a, Facultad de Biologi\u0301a, Universidad de Barcelona, 08071 Barcelona"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.161.3.955-962.1985"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.155.2.831-838.1983"},{"key":"e_1_3_2_5_2","first-page":"831","volume":"59","year":"1977","unstructured":"Erbing, C., B. 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