{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,18]],"date-time":"2026-07-18T17:10:28Z","timestamp":1784394628658,"version":"3.55.0"},"reference-count":39,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2003,3,15]],"date-time":"2003-03-15T00:00:00Z","timestamp":1047686400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2003,3,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Phosphoglyceride-linked enterobacterial common antigen (ECA\n            <jats:sub>PG<\/jats:sub>\n            ) is a cell surface glycolipid that is synthesized by all gram-negative enteric bacteria. The carbohydrate portion of ECA\n            <jats:sub>PG<\/jats:sub>\n            consists of linear heteropolysaccharide chains comprised of the trisaccharide repeat unit Fuc4NAc-ManNAcA-GlcNAc, where Fuc4NAc is 4-acetamido-4,6-dideoxy-\n            <jats:sc>d<\/jats:sc>\n            -galactose, ManNAcA is\n            <jats:italic>N<\/jats:italic>\n            -acetyl-\n            <jats:sc>d<\/jats:sc>\n            -mannosaminuronic acid, and GlcNAc is\n            <jats:italic>N<\/jats:italic>\n            -acetyl-\n            <jats:sc>d<\/jats:sc>\n            -glucosamine. The potential reducing terminal GlcNAc residue of each polysaccharide chain is linked via phosphodiester linkage to a phosphoglyceride aglycone. We demonstrate here the occurrence of a water-soluble cyclic form of enterobacterial common antigen, ECA\n            <jats:sub>CYC<\/jats:sub>\n            , purified from\n            <jats:italic>Escherichia coli<\/jats:italic>\n            strains B and K-12 with solution nuclear magnetic resonance (NMR) spectroscopy, electrospray ionization mass spectrometry (ESI-MS), and additional biochemical methods. The ECA\n            <jats:sub>CYC<\/jats:sub>\n            molecules lacked an aglycone and contained four trisaccharide repeat units that were nonstoichiometrically substituted with up to four O-acetyl groups. ECA\n            <jats:sub>CYC<\/jats:sub>\n            was not detected in mutant strains that possessed null mutations in the\n            <jats:italic>wecA<\/jats:italic>\n            ,\n            <jats:italic>wecF<\/jats:italic>\n            , and\n            <jats:italic>wecG<\/jats:italic>\n            genes of the\n            <jats:italic>wec<\/jats:italic>\n            gene cluster. These observations corroborate the structural data obtained by NMR and ESI-MS analyses and show for the first time that the trisaccharide repeat units of ECA\n            <jats:sub>CYC<\/jats:sub>\n            and ECA\n            <jats:sub>PG<\/jats:sub>\n            are assembled by a common biosynthetic pathway.\n          <\/jats:p>","DOI":"10.1128\/jb.185.6.1995-2004.2003","type":"journal-article","created":{"date-parts":[[2003,3,4]],"date-time":"2003-03-04T19:35:53Z","timestamp":1046806553000},"page":"1995-2004","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":59,"title":["Identification and Biosynthesis of Cyclic Enterobacterial Common Antigen in\n            <i>Escherichia coli<\/i>"],"prefix":"10.1128","volume":"185","author":[{"given":"Paul J. A.","family":"Erbel","sequence":"first","affiliation":[{"name":"Department of Biochemistry"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathleen","family":"Barr","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, F. Edward He\u0301bert School of Medicine, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ninguo","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75390-9038"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerrit J.","family":"Gerwig","sequence":"additional","affiliation":[{"name":"Department of Bio-Organic Chemistry, Bijvoet Center, Utrecht University, 3508 TB Utrecht, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul D.","family":"Rick","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, F. Edward He\u0301bert School of Medicine, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin H.","family":"Gardner","sequence":"additional","affiliation":[{"name":"Department of Biochemistry"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.168.1.348-356.1986"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.147.2.602-611.1981"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.181.20.6564-6568.1999"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.171.3.1326-1332.1989"},{"key":"e_1_3_2_6_2","doi-asserted-by":"crossref","first-page":"7142","DOI":"10.1016\/S0021-9258(18)48216-6","volume":"262","year":"1987","unstructured":"Barr, K., and P. D. Rick. 1987. Biosynthesis of enterobacterial common antigen in Escherichia coli. 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