{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T04:55:33Z","timestamp":1791348933402,"version":"4.3.1"},"reference-count":32,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2003,4,15]],"date-time":"2003-04-15T00:00:00Z","timestamp":1050364800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2003,4,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Interaction with host cells is essential in meningococcal pathogenesis especially at the blood-brain barrier. This step is likely to involve a common regulatory pathway allowing coordinate regulation of genes necessary for the interaction with endothelial cells. The analysis of the genomic sequence of<jats:italic>Neisseria meningitidis<\/jats:italic>Z2491 revealed the presence of many repeats. One of these, designated REP2, contains a \u221224\/\u221212 type promoter and a ribosome binding site 5 to 13 bp before an ATG. In addition most of these REP2 sequences are located immediately upstream of an ORF. Among these REP2-associated genes are<jats:italic>pilC1<\/jats:italic>and<jats:italic>crgA<\/jats:italic>, described as being involved in steps essential for the interaction of<jats:italic>N. meningitidis<\/jats:italic>with host cells. Furthermore, the REP2 sequences located upstream of<jats:italic>pilC1<\/jats:italic>and<jats:italic>crgA<\/jats:italic>correspond to the previously identified promoters known to be induced during the initial localized adhesion of<jats:italic>N. meningitidis<\/jats:italic>with human cells. This characteristic led us to hypothesize that at least some of the REP2-associated genes were upregulated under the same circumstances as<jats:italic>pilC1<\/jats:italic>and<jats:italic>crgA<\/jats:italic>. Quantitative PCR in real time demonstrated that the expression of 14 out of 16 REP2-associated genes were upregulated during the initial localized adhesion of<jats:italic>N. meningitidis<\/jats:italic>. Taken together, these data suggest that these repeats control a set of genes necessary for the efficient interaction of this pathogen with host cells. Subsequent mutational analysis was performed to address the role of these genes during meningococcus-cell interaction.<\/jats:p>","DOI":"10.1128\/jb.185.8.2618-2627.2003","type":"journal-article","created":{"date-parts":[[2003,4,1]],"date-time":"2003-04-01T14:27:29Z","timestamp":1049207249000},"page":"2618-2627","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":40,"title":["The REP2 Repeats of the Genome of<i>Neisseria meningitidis<\/i>Are Associated with Genes Coordinately Regulated during Bacterial Cell Interaction"],"prefix":"10.1128","volume":"185","author":[{"given":"Sandrine","family":"Morelle","sequence":"first","affiliation":[{"name":"INSERM U570, Faculte\u0301 de Me\u0301decine Necker-Enfants Malades, Universite\u0301 Rene\u0301 Descartes, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Etienne","family":"Carbonnelle","sequence":"additional","affiliation":[{"name":"INSERM U570, Faculte\u0301 de Me\u0301decine Necker-Enfants Malades, Universite\u0301 Rene\u0301 Descartes, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xavier","family":"Nassif","sequence":"additional","affiliation":[{"name":"INSERM U570, Faculte\u0301 de Me\u0301decine Necker-Enfants Malades, Universite\u0301 Rene\u0301 Descartes, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","unstructured":"Barrios H. B. Valderrama and E. Morett. 1999. Compilation and analysis of sigma 54 -dependent promoter sequences. Nucleic Acids Res. 27: 4305-4313.","DOI":"10.1093\/nar\/27.22.4305"},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"12194","DOI":"10.1016\/S0021-9258(18)37738-X","volume":"263","year":"1988","unstructured":"Correia, F. F., S. Inouye, and M. Inouye. 1988. A family of small repeated elements with some transposon-like properties in the genome of Neisseria gonorrhoeae. J. Biol. Chem.263:12194-12198.","journal-title":"J. Biol. Chem."},{"key":"e_1_3_2_4_2","doi-asserted-by":"crossref","first-page":"1555","DOI":"10.1046\/j.1365-2958.2002.02838.x","volume":"43","year":"2002","unstructured":"Deghmane, A. E., D. Giorgini, M. Larribe, J. M. Alonso, and M. K. Taha. 2002. Down-regulation of pili and capsule of Neisseria meningitidis upon contact with epithelial cells is mediated by CrgA regulatory protein. Mol. Microbiol.43:1555-1564.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_5_2","doi-asserted-by":"crossref","first-page":"1068","DOI":"10.1093\/emboj\/19.5.1068","volume":"19","year":"2000","unstructured":"Deghmane, A. E., S. Petit, A. Topilko, Y. Pereira, D. Giorgini, M. Larribe, and M. K. Taha. 2000. Intimate adhesion of Neisseria meningitidis to human epithelial cells is under the control of the crgA gene, a novel LysR-type transcriptional regulator. EMBO J.19:1068-1078.","journal-title":"EMBO J."},{"key":"e_1_3_2_6_2","doi-asserted-by":"crossref","first-page":"1231","DOI":"10.1242\/jcs.115.6.1231","volume":"115","year":"2002","unstructured":"Eugene, E., I. Hoffmann, C. Pujol, P. O. Couraud, S. Bourdoulous, and X. Nassif. 2002. Microvilli-like structures are associated with the internalization of virulent capsulated Neisseria meningitidis into vascular endothelial cells. J. Cell Sci.115:1231-1241.","journal-title":"J. Cell Sci."},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.173.18.5921-5923.1991"},{"key":"e_1_3_2_8_2","doi-asserted-by":"crossref","first-page":"6982","DOI":"10.1073\/pnas.85.18.6982","volume":"85","year":"1988","unstructured":"Goodman, S. D., and J. J. Scocca. 1988. Identification and arrangement of the DNA sequence recognized in specific transformation of Neisseria gonorrhoeae. Proc. Natl. Acad. Sci. USA85:6982-6986.","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"e_1_3_2_9_2","doi-asserted-by":"crossref","first-page":"914","DOI":"10.1038\/nbt728","volume":"20","year":"2002","unstructured":"Grifantini, R., E. Bartolini, A. Muzzi, M. Draghi, E. Frigimelica, J. Berger, G. Ratti, R. Petracca, G. Galli, M. Agnusdei, M. Monica Giuliani, L. Santini, B. Brunelli, H. Tettelin, R. Rappuoli, F. Randazzo, and G. Grandi. 2002. Previously unrecognized vaccine candidates against group B meningococcus identified by DNA microarrays. Nat. Biotechnol.20:914-921.","journal-title":"Nat. Biotechnol."},{"key":"e_1_3_2_10_2","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1146\/annurev.genet.33.1.193","volume":"33","year":"1999","unstructured":"Grunberg-Manago, M. 1999. Messenger RNA stability and its role in control of gene expression in bacteria and phages. Annu. Rev. Genet.33:193-227.","journal-title":"Annu. Rev. Genet."},{"key":"e_1_3_2_11_2","first-page":"3","volume":"72","year":"1988","unstructured":"Higgins, C. F., R. S. McLaren, and S. F. Newbury. 1988. Repetitive extragenic palindromic sequences, mRNA stability and gene expression: evolution by gene conversion? A review. Gene72:3-14.","journal-title":"A review. Gene"},{"key":"e_1_3_2_12_2","doi-asserted-by":"crossref","first-page":"1274","DOI":"10.1128\/jb.85.6.1274-1279.1963","volume":"85","year":"1963","unstructured":"Kellogg, D. S., W. L. Peacock, W. E. Deacon, L. Brown, and C. I. Pirkle. 1963. Neisseria gnonorrhoeae. I. Virulence genetically linked to clonal variation. J. Bacteriol.85:1274-1279.","journal-title":"J. Bacteriol."},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.4.2082-2095.2000"},{"key":"e_1_3_2_14_2","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/S0378-1119(97)00664-1","volume":"208","year":"1998","unstructured":"Laskos, L., J. P. Dillard, H. S. Seifert, J. A. Fyfe, and J. K. Davies. 1998. The pathogenic neisseriae contain an inactive rpoN gene and do not utilize the pilE sigma54 promoter. Gene208:95-102.","journal-title":"Gene"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.184.22.6163-6173.2002"},{"key":"e_1_3_2_16_2","doi-asserted-by":"crossref","first-page":"1316","DOI":"10.1046\/j.1365-2958.1999.01459.x","volume":"32","year":"1999","unstructured":"Merz, A. J., C. A. Enns, and M. So. 1999. Type IV pili of pathogenic Neisseriae elicit cortical plaque formation in epithelial cells. Mol. Microbiol.32:1316-1332.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.65.10.4341-4349.1997"},{"key":"e_1_3_2_18_2","doi-asserted-by":"crossref","first-page":"846","DOI":"10.1046\/j.1365-2958.2001.02452.x","volume":"40","year":"2001","unstructured":"Morand, P. C., P. Tattevin, E. Eugene, J. L. Beretti, and X. Nassif. 2001. The adhesive property of the type IV pilus-associated component PilC1 of pathogenic Neisseria is supported by the conformational structure of the N-terminal part of the molecule. Mol. Microbiol.40:846-856.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_19_2","doi-asserted-by":"crossref","first-page":"3769","DOI":"10.1073\/pnas.91.9.3769","volume":"91","year":"1994","unstructured":"Nassif, X., J. L. Beretti, J. Lowy, P. Stenberg, P. O'Gaora, J. Pfeifer, S. Normark, and M. So. 1994. Roles of pilin and PilC in adhesion of Neisseria meningitidis to human epithelial and endothelial cells. Proc. Natl. Acad. Sci. USA91:3769-3773.","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"e_1_3_2_20_2","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1111\/j.1365-2958.1993.tb01615.x","volume":"8","year":"1993","unstructured":"Nassif, X., J. Lowy, P. Stenberg, P. O'Gaora, A. Ganji, and M. So. 1993. Antigenic variation of pilin regulates adhesion of Neisseria meningitidis to human epithelial cells. Mol. Microbiol.8:719-725.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/35006655"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.19.5391-5398.2000"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.65.11.4836-4842.1997"},{"key":"e_1_3_2_24_2","doi-asserted-by":"crossref","first-page":"4017","DOI":"10.1073\/pnas.96.7.4017","volume":"96","year":"1999","unstructured":"Pujol, C., E. Eugene, M. Marceau, and X. Nassif. 1999. The meningococcal PilT protein is required for induction of intimate attachment to epithelial cells following pilus-mediated adhesion. Proc. Natl. Acad. Sci. USA96:4017-4022.","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"e_1_3_2_25_2","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/S0378-1119(98)00374-6","volume":"217","year":"1998","unstructured":"Salzberg, S. L., A. J. Salzberg, A. R. Kerlavage, and J. F. Tomb. 1998. Skewed oligomers and origins of replication. Gene217:57-67.","journal-title":"Gene"},{"key":"e_1_3_2_26_2","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1111\/j.1365-2958.1994.tb00416.x","volume":"13","year":"1994","unstructured":"Sarkari, J., N. Pandit, E. R. Moxon, and M. Achtman. 1994. Variable expression of the Opc outer membrane protein in Neisseria meningitidis is caused by size variation of a promoter containing poly-cytidine. Mol. Microbiol13:207-217.","journal-title":"Mol. Microbiol"},{"key":"e_1_3_2_27_2","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1146\/annurev.mi.47.100193.003121","volume":"47","year":"1993","unstructured":"Schell, M. A. 1993. Molecular biology of the LysR family of transcriptional regulators. Annu. Rev. Microbiol.47:597-626.","journal-title":"Annu. Rev. Microbiol."},{"key":"e_1_3_2_28_2","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1006\/abio.2000.4753","volume":"285","year":"2000","unstructured":"Schmittgen, T. D., B. A. Zakrajsek, A. G. Mills, V. Gorn, M. J. Singer, and M. W. Reed. 2000. Quantitative reverse transcription-polymerase chain reaction to study mRNA decay: comparison of endpoint and real-time methods. Anal. Biochem.285:194-204.","journal-title":"Anal. Biochem."},{"key":"e_1_3_2_29_2","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1111\/j.1365-2958.1995.tb02376.x","volume":"15","year":"1995","unstructured":"Taha, M. K., and D. Giorgini. 1995. Phosphorylation and functional analysis of PilA, a protein involved in the transcriptional regulation of the pilin gene in Neisseria gonorrhoeae.Mol. Microbiol.15:667-677.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_30_2","doi-asserted-by":"crossref","first-page":"1073","DOI":"10.1046\/j.1365-2958.1996.448979.x","volume":"19","year":"1996","unstructured":"Taha, M. K., D. Giorgini, and X. Nassif. 1996. The pilA regulatory gene modulates the pilus-mediated adhesion of Neisseria meningitidis by controlling the transcription of pilC1. Mol. Microbiol.19:1073-1084.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_31_2","doi-asserted-by":"crossref","first-page":"1153","DOI":"10.1046\/j.1365-2958.1998.00876.x","volume":"28","year":"1998","unstructured":"Taha, M. K., P. C. Morand, Y. Pereira, E. Eugene, D. Giorgini, M. Larribe, and X. Nassif. 1998. Pilus-mediated adhesion of Neisseria meningitidis: the essential role of cell contact-dependent transcriptional upregulation of the PilC1 protein. Mol. Microbiol.28:1153-1163.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_32_2","doi-asserted-by":"crossref","first-page":"1809","DOI":"10.1126\/science.287.5459.1809","volume":"287","year":"2000","unstructured":"Tettelin, H., N. J. Saunders, J. Heidelberg, A. C. Jeffries, K. E. Nelson, J. A. Eisen, K. A. Ketchum, D. W. Hood, J. F. Peden, R. J. Dodson, W. C. Nelson, M. L. Gwinn, R. DeBoy, J. D. Peterson, E. K. Hickey, D. H. Haft, S. L. Salzberg, O. White, R. D. Fleischmann, B. A. Dougherty, T. Mason, A. Ciecko, D. S. Parksey, E. Blair, H. Cittone, E. B. Clark, M. D. Cotton, T. R. Utterback, H. Khouri, H. Qin, J. Vamathevan, J. Gill, V. Scarlato, V. Masignani, M. Pizza, G. Grandi, L. Sun, H. O. Smith, C. M. Fraser, E. R. Moxon, R. Rappuoli, and J. C. Venter. 2000. Complete genome sequence of Neisseria meningitidis serogroup B strain MC58. Science287:1809-1815.","journal-title":"Science"},{"key":"e_1_3_2_33_2","first-page":"341","volume":"5","year":"1989","unstructured":"Thony, B., and H. Hennecke. 1989. The \u221224\/\u221212 promoter comes of age. FEMS Microbiol. Rev.5:341-357.","journal-title":"FEMS Microbiol. Rev."}],"container-title":["Journal of Bacteriology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JB.185.8.2618-2627.2003","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JB.185.8.2618-2627.2003","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,9]],"date-time":"2024-01-09T10:38:23Z","timestamp":1704796703000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JB.185.8.2618-2627.2003"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,4,15]]},"references-count":32,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2003,4,15]]}},"alternative-id":["10.1128\/JB.185.8.2618-2627.2003"],"URL":"https:\/\/doi.org\/10.1128\/jb.185.8.2618-2627.2003","relation":{},"ISSN":["0021-9193","1098-5530"],"issn-type":[{"value":"0021-9193","type":"print"},{"value":"1098-5530","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,4,15]]},"assertion":[{"value":"2002-09-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2003-01-08","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-20","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}