{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T02:17:05Z","timestamp":1791166625822,"version":"4.1.0"},"reference-count":23,"publisher":"American Society for Microbiology","issue":"24","license":[{"start":{"date-parts":[[2004,12,15]],"date-time":"2004-12-15T00:00:00Z","timestamp":1103068800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2004,12,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            This paper provides the biochemical evidence for physical interactions between the outer membrane component, TolC, and the membrane fusion protein component, AcrA, of the major antibiotic efflux pump of\n            <jats:italic>Escherichia coli<\/jats:italic>\n            . Cross-linking between TolC and AcrA was independent of the presence of any externally added substrate of the efflux pump or of the pump protein, AcrB. The biochemical demonstration of a TolC-AcrA interaction is consistent with genetic studies in which extragenic suppressors of a mutant TolC strain were found in the\n            <jats:italic>acrA<\/jats:italic>\n            gene.\n          <\/jats:p>","DOI":"10.1128\/jb.186.24.8533-8536.2004","type":"journal-article","created":{"date-parts":[[2004,12,2]],"date-time":"2004-12-02T23:48:13Z","timestamp":1102031293000},"page":"8533-8536","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":55,"title":["Interaction between the TolC and AcrA Proteins of a Multidrug Efflux System of\n            <i>Escherichia coli<\/i>"],"prefix":"10.1128","volume":"186","author":[{"given":"Fasahath","family":"Husain","sequence":"first","affiliation":[{"name":"Microbiology Graduate Program"},{"name":"School of Life Sciences, Arizona State University, Tempe, Arizona"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew","family":"Humbard","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Arizona State University, Tempe, Arizona"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rajeev","family":"Misra","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Arizona State University, Tempe, Arizona"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.C400164200"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.6.1851-1860.2004"},{"key":"e_1_3_2_4_2","first-page":"541","volume":"141","year":"1976","unstructured":"Casadaban, M. J. 1976. Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and Mu. J. Mol. Biol.141:541-555.","journal-title":"J. Mol. Biol."},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.120163297"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.176.13.3825-3831.1994"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.178.19.5803-5805.1996"},{"key":"e_1_3_2_8_2","doi-asserted-by":"crossref","first-page":"620","DOI":"10.1111\/j.1365-2958.2004.04301.x","volume":"54","year":"2004","unstructured":"Gerken, H., and R. Misra. 2004. Genetic evidence for AcrB-dependent functional interactions between TolC and AcrA proteins of a major antibiotic efflux pump of Escherichia coli. Mol. Microbiol.54:620-631.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_9_2","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1006\/jmbi.2001.4578","volume":"308","year":"2001","unstructured":"German, G. J., and R. Misra. 2001. The TolC protein of Escherichia coli serves as a cell-surface receptor for the newly characterized TLS bacteriophage. J. Mol. Biol.308:579-585.","journal-title":"J. Mol. Biol."},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.177.14.4121-4130.1995"},{"key":"e_1_3_2_11_2","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1093\/oxfordjournals.jbchem.a022741","volume":"128","year":"2000","unstructured":"Kawabe, T., E. Fujihira, and A. Yamaguchi. 2000. Molecular construction of a multidrug exporter system, AcrAB: molecular interaction between AcrA and AcrB, and cleavage of the N-terminal signal sequence of AcrA. J. Biochem.128:195-200.","journal-title":"J. Biochem."},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/35016007"},{"key":"e_1_3_2_13_2","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1038\/nature01050","volume":"419","year":"2002","unstructured":"Murakami, S., R. Nakashima, E. Yamashita, and A. Yamaguchi. 2002. Crystal structure of bacterial multidrug efflux transporter AcrB. Nature419:587-593.","journal-title":"Nature"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.94.4.1112-1123.1967"},{"key":"e_1_3_2_15_2","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1126\/science.8153625","volume":"264","year":"1994","unstructured":"Nikaido, H. 1994. Prevention of drug access to bacterial targets: permeability barriers and active efflux. Science264:382-388.","journal-title":"Science"},{"key":"e_1_3_2_16_2","first-page":"215","volume":"3","year":"2001","unstructured":"Nikaido, H., and H. I. Zgurskaya. 2001. AcrAB and related multidrug efflux pumps of Escherichia coli. J. Mol. Microbiol. Biotechnol.3:215-218.","journal-title":"J. Mol. Microbiol. Biotechnol."},{"key":"e_1_3_2_17_2","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1111\/j.1365-2958.1994.tb00362.x","volume":"11","year":"1994","unstructured":"Saier, M. H., Jr., R. Tam, A. Reizer, and J. Reizer. 1994. Two novel families of bacterial membrane proteins concerned with nodulation, cell division and transport. Mol. Microbiol.11:841-847.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/17.22.6487"},{"key":"e_1_3_2_19_2","doi-asserted-by":"crossref","first-page":"4746","DOI":"10.1073\/pnas.87.12.4746","volume":"87","year":"1990","unstructured":"Wandersman, C., and P. Delepelaire. 1990. TolC, an Escherichia coli outer membrane protein required for hemolysin secretion. Proc. Natl. Acad. Sci. USA87:4746-4780.","journal-title":"Proc. Natl. Acad. Sci. 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