{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,12]],"date-time":"2026-01-12T20:13:18Z","timestamp":1768248798678,"version":"3.49.0"},"reference-count":51,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2004,2,15]],"date-time":"2004-02-15T00:00:00Z","timestamp":1076803200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2004,2,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The gene cluster (\n            <jats:italic>waa<\/jats:italic>\n            ) involved in\n            <jats:italic>Serratia marcescens<\/jats:italic>\n            N28b core lipopolysaccharide (LPS) biosynthesis was identified, cloned, and sequenced. Complementation analysis of known\n            <jats:italic>waa<\/jats:italic>\n            mutants from\n            <jats:italic>Escherichia coli<\/jats:italic>\n            K-12,\n            <jats:italic>Salmonella enterica<\/jats:italic>\n            , and\n            <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n            led to the identification of five genes coding for products involved in the biosynthesis of a shared inner core structure: [\n            <jats:sc>l<\/jats:sc>\n            ,\n            <jats:sc>d<\/jats:sc>\n            -Hep\n            <jats:italic>p<\/jats:italic>\n            III\u03b1(1\u21927)-\n            <jats:sc>l<\/jats:sc>\n            ,\n            <jats:sc>d<\/jats:sc>\n            -Hep\n            <jats:italic>p<\/jats:italic>\n            II\u03b1(1\u21923)-\n            <jats:sc>l,d<\/jats:sc>\n            -Hep\n            <jats:italic>p<\/jats:italic>\n            I\u03b1(1\u21925)-Kdo\n            <jats:italic>p<\/jats:italic>\n            I(4\u21902)\u03b1Kdo\n            <jats:italic>p<\/jats:italic>\n            II] (\n            <jats:sc>l,d<\/jats:sc>\n            -Hep\n            <jats:italic>p<\/jats:italic>\n            ,\n            <jats:sc>l<\/jats:sc>\n            -\n            <jats:italic>glycero<\/jats:italic>\n            -\n            <jats:sc>d<\/jats:sc>\n            -\n            <jats:italic>manno<\/jats:italic>\n            -heptopyranose; Kdo, 3-deoxy-\n            <jats:sc>d<\/jats:sc>\n            -\n            <jats:italic>manno<\/jats:italic>\n            -oct-2-ulosonic acid). Complementation and\/or chemical analysis of several nonpolar mutants within the\n            <jats:italic>S. marcescens waa<\/jats:italic>\n            gene cluster suggested that in addition, three\n            <jats:italic>waa<\/jats:italic>\n            genes were shared by\n            <jats:italic>S. marcescens<\/jats:italic>\n            and\n            <jats:italic>K. pneumoniae<\/jats:italic>\n            , indicating that the core region of the LPS of\n            <jats:italic>S. marcescens<\/jats:italic>\n            and\n            <jats:italic>K. pneumoniae<\/jats:italic>\n            possesses additional common features. Chemical and structural analysis of the major oligosaccharide from the core region of LPS of an O-antigen-deficient mutant of\n            <jats:italic>S. marcescens<\/jats:italic>\n            N28b as well as complementation analysis led to the following proposed structure: \u03b2-Glc-(1\u21926)-\u03b1-Glc-(1\u21924))-\u03b1-\n            <jats:sc>d<\/jats:sc>\n            -GlcN-(1\u21924)-\u03b1-\n            <jats:sc>d<\/jats:sc>\n            -GalA-[(2\u21901)-\u03b1-\n            <jats:sc>d,d<\/jats:sc>\n            -Hep-(2\u21901)-\u03b1-Hep]-(1\u21923)-\u03b1-\n            <jats:sc>l,d<\/jats:sc>\n            -Hep[(7\u21901)-\u03b1-\n            <jats:sc>l,d<\/jats:sc>\n            -Hep]-(1\u21923)-\u03b1-\n            <jats:sc>l,d<\/jats:sc>\n            -Hep-[(4\u21901)-\u03b2-\n            <jats:sc>d<\/jats:sc>\n            -Glc]-(1\u21925)-Kdo. The D configuration of the \u03b2-Glc, \u03b1-GclN, and \u03b1-GalA residues was deduced from genetic data and thus is tentative. Furthermore, other oligosaccharides were identified by ion cyclotron resonance-Fourier-transformed electrospray ionization mass spectrometry, which presumably contained in addition one residue of\n            <jats:sc>d<\/jats:sc>\n            -glycero-\n            <jats:sc>d<\/jats:sc>\n            -\n            <jats:italic>talo<\/jats:italic>\n            -oct-2-ulosonic acid (Ko) or of a hexuronic acid. Several ions were identified that differed from others by a mass of +80 Da, suggesting a nonstoichiometric substitution by a monophosphate residue. However, none of these molecular species could be isolated in substantial amounts and structurally analyzed. On the basis of the structure shown above and the analysis of nonpolar mutants, functions are suggested for the genes involved in core biosynthesis.\n          <\/jats:p>","DOI":"10.1128\/jb.186.4.978-988.2004","type":"journal-article","created":{"date-parts":[[2004,2,4]],"date-time":"2004-02-04T23:24:31Z","timestamp":1075937071000},"page":"978-988","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":22,"title":["Genetic and Structural Characterization of the Core Region of the Lipopolysaccharide from\n            <i>Serratia marcescens<\/i>\n            N28b (Serovar O4)"],"prefix":"10.1128","volume":"186","author":[{"given":"Nu\u0301ria","family":"Coderch","sequence":"first","affiliation":[{"name":"Departamento de Microbiologi\u0301a y Parasitologi\u0301a Sanitarias, Facultad de Farmacia, Universidada de Barcelona, 08028 Barcelona"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nu\u0301ria","family":"Pique\u0301","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologi\u0301a y Parasitologi\u0301a Sanitarias, Facultad de Farmacia, Universidada de Barcelona, 08028 Barcelona"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Buko","family":"Lindner","sequence":"additional","affiliation":[{"name":"Division of Biophysics"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nihal","family":"Abitiu","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologi\u0301a y Parasitologi\u0301a Sanitarias, Facultad de Farmacia, Universidada de Barcelona, 08028 Barcelona"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susana","family":"Merino","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologi\u0301a, Facultad de Biologi\u0301a, Universidad de Barcelona, 08071 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luis","family":"Izquierdo","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologi\u0301a, Facultad de Biologi\u0301a, Universidad de Barcelona, 08071 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natalia","family":"Jimenez","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologi\u0301a, Facultad de Biologi\u0301a, Universidad de Barcelona, 08071 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan M.","family":"Toma\u0301s","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologi\u0301a, Facultad de Biologi\u0301a, Universidad de Barcelona, 08071 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Otto","family":"Holst","sequence":"additional","affiliation":[{"name":"Division of Structural Biochemistry, Research Center Borstel, Leibnitz Center for Medicine and Biosciences, D-23845 Leibnitz, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miguel","family":"Regue\u0301","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologi\u0301a y Parasitologi\u0301a Sanitarias, Facultad de Farmacia, Universidada de Barcelona, 08028 Barcelona"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1017\/S0195941700064201","volume":"7","year":"1986","unstructured":"Acar, J. 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