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The<jats:italic>srtA<\/jats:italic>gene product anchors internalin and some other LPXTG-containing proteins to the listerial surface. Here, we focus on the role of the second sortase, SrtB. Whereas SrtA acts on most of the proteins in the peptidoglycan fraction, SrtB appears to target minor amounts of surface polypeptides. We identified one of the SrtB-anchored proteins as the virulence factor SvpA, a surface-exposed protein which does not contain the LPXTG motif. Therefore, as in<jats:italic>Staphylococcus aureus<\/jats:italic>, the listerial SrtB represents a second class of sortase in<jats:italic>L. monocytogenes<\/jats:italic>, involved in the attachment of a subset of proteins to the cell wall, most likely by recognizing an NXZTN sorting motif. The \u0394<jats:italic>srtB<\/jats:italic>mutant strain does not have defects in bacterial entry, growth, or motility in tissue-cultured cells and does not show attenuated virulence in mice. SrtB-mediated anchoring could therefore be required to anchor surface proteins involved in the adaptation of this microorganism to different environmental conditions.<\/jats:p>","DOI":"10.1128\/jb.186.7.1972-1982.2004","type":"journal-article","created":{"date-parts":[[2004,3,17]],"date-time":"2004-03-17T20:13:08Z","timestamp":1079554388000},"page":"1972-1982","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":88,"title":["Sortase B, a New Class of Sortase in<i>Listeria monocytogenes<\/i>"],"prefix":"10.1128","volume":"186","author":[{"given":"He\u0301le\u0300ne","family":"Bierne","sequence":"first","affiliation":[{"name":"Unite\u0301 des Interactions Bacte\u0301ries-Cellules, Institut Pasteur, 75724 Paris Cedex 15"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline","family":"Garandeau","sequence":"additional","affiliation":[{"name":"INSERM U-570, CHU Necker-Enfants Malades, 75730 Paris Cedex 15, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. 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