{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T08:48:33Z","timestamp":1790930913154,"version":"4.1.0"},"reference-count":46,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2005,6,15]],"date-time":"2005-06-15T00:00:00Z","timestamp":1118793600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2005,6,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Cytochrome\n            <jats:italic>c<\/jats:italic>\n            \u2032 (Cyt\n            <jats:italic>c<\/jats:italic>\n            \u2032) is a\n            <jats:italic>c<\/jats:italic>\n            -type cytochrome with a pentacoordinate heme iron. The gene encoding this protein in\n            <jats:italic>Rhodobacter sphaeroides<\/jats:italic>\n            2.4.3, designated\n            <jats:italic>cycP<\/jats:italic>\n            , was isolated and sequenced. Northern blot analysis and \u03b2-galactosidase assays demonstrated that\n            <jats:italic>cycP<\/jats:italic>\n            transcription increased as oxygen levels decreased and was not repressed under denitrifying conditions as observed in another\n            <jats:italic>Rhodobacter<\/jats:italic>\n            species. CO difference spectra performed with extracts of cells grown under different conditions revealed that Cyt\n            <jats:italic>c<\/jats:italic>\n            \u2032 levels were highest during photosynthetic denitrifying growth conditions. The increase in Cyt\n            <jats:italic>c<\/jats:italic>\n            \u2032 under this condition was higher than would be predicted from transcriptional studies. Electron paramagnetic resonance analysis of whole cells demonstrated that Cyt\n            <jats:italic>c<\/jats:italic>\n            \u2032 binds NO during denitrification. Mass spectrometric analysis of nitrogen oxides produced by cells and purified protein did not indicate that Cyt\n            <jats:italic>c<\/jats:italic>\n            \u2032 has NO reductase activity. Taken together, these results suggest a model where Cyt\n            <jats:italic>c<\/jats:italic>\n            \u2032 in\n            <jats:italic>R. sphaeroides<\/jats:italic>\n            2.4.3 may shuttle NO to the membrane, where it can be reduced.\n          <\/jats:p>","DOI":"10.1128\/jb.187.12.4077-4085.2005","type":"journal-article","created":{"date-parts":[[2005,6,3]],"date-time":"2005-06-03T16:03:36Z","timestamp":1117814616000},"page":"4077-4085","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":28,"title":["Regulation and Function of Cytochrome\n            <i>c<\/i>\n            \u2032 in\n            <i>Rhodobacter sphaeroides<\/i>\n            2.4.3"],"prefix":"10.1128","volume":"187","author":[{"given":"Peter S.","family":"Choi","sequence":"first","affiliation":[{"name":"Department of Microbiology, Wing Hall, Cornell University, Ithaca, New York 14853"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vladimir M.","family":"Grigoryants","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Center for Biophysics and Biochemistry, University at Albany, State University of New York at Albany, Albany, New York 12222"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hector D.","family":"Abrun\u0303a","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Chemical Biology, Baker Laboratory, Cornell University, Ithaca, New York 14853"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles P.","family":"Scholes","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Center for Biophysics and Biochemistry, University at Albany, State University of New York at Albany, Albany, New York 12222"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James P.","family":"Shapleigh","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Wing Hall, Cornell University, Ithaca, New York 14853"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"6854","DOI":"10.1073\/pnas.78.11.6854","volume":"78","year":"1981","unstructured":"Ambler, R. 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