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The identity of the mutant was initially determined from its amino acid sequence, which is 68% identical to\n            <jats:italic>Escherichia coli<\/jats:italic>\n            and 77% identical to\n            <jats:italic>Brucella melitensis<\/jats:italic>\n            . The mutant lost all CS enzymatic activity, and a cloned CS gene complemented a CS mutation in\n            <jats:italic>Sinorhizobium<\/jats:italic>\n            . The CS mutation resulted in a 10-fold reduction in\n            <jats:italic>vir<\/jats:italic>\n            gene expression, which likely accounts for the attenuated virulence. When a plasmid containing a constitutive\n            <jats:italic>virG<\/jats:italic>\n            [\n            <jats:italic>virG<\/jats:italic>\n            (Con)] locus was introduced into this mutant, the level of\n            <jats:italic>vir<\/jats:italic>\n            gene induction was restored to nearly wild-type level. Further, the\n            <jats:italic>virG<\/jats:italic>\n            (Con)-complemented CS mutant strain induced tumors that were similar in size and number to those induced by the parental strain. The CS mutation resulted in only a minor reduction in growth rate in a glucose-salts medium. Both the CS mutant and the\n            <jats:italic>virG<\/jats:italic>\n            (Con)-complemented CS strain displayed similar growth deficiencies in a glucose-salts medium, indicating that the reduced growth rate of the CS mutant could not be responsible for the attenuated virulence. A search of the genome of\n            <jats:italic>A. tumefaciens<\/jats:italic>\n            C58 revealed four proteins, encoded on different replicons, with conserved CS motifs. However, only the locus that when mutated resulted in an attenuated phenotype has CS activity. Mutations in the other three loci did not result in attenuated virulence and any loss of CS activity, and none were able to complement the CS mutation in\n            <jats:italic>Sinorhizobium<\/jats:italic>\n            . The function of these loci remains unknown.\n          <\/jats:p>","DOI":"10.1128\/jb.187.14.4844-4852.2005","type":"journal-article","created":{"date-parts":[[2005,7,2]],"date-time":"2005-07-02T05:38:58Z","timestamp":1120282738000},"page":"4844-4852","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":15,"title":["Citrate Synthase Mutants of\n            <i>Agrobacterium<\/i>\n            Are Attenuated in Virulence and Display Reduced\n            <i>vir<\/i>\n            Gene Induction"],"prefix":"10.1128","volume":"187","author":[{"given":"Maneewan","family":"Suksomtip","sequence":"first","affiliation":[{"name":"Department of Microbiology, Box 357242, University of Washington, Seattle, Washington 98195-7242"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pu","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Box 357242, University of Washington, Seattle, Washington 98195-7242"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tamara","family":"Anderson","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Box 357242, University of Washington, Seattle, Washington 98195-7242"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sumalee","family":"Tungpradabkul","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Box 357242, University of Washington, Seattle, Washington 98195-7242"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Derek W.","family":"Wood","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Box 357242, University of Washington, Seattle, Washington 98195-7242"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eugene W.","family":"Nester","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Box 357242, University of Washington, Seattle, Washington 98195-7242"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"67","year":"1993","unstructured":"Ankenbauer, R. 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