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Genes for the transport of heme into the bacterial cell have been identified, but little is known about proteins that control the fate of the heme molecule after it has entered the cell. The<jats:italic>shuS<\/jats:italic>gene is located within the heme transport locus, downstream of the heme receptor gene<jats:italic>shuA<\/jats:italic>. ShuS is a heme binding protein, but its role in heme utilization is poorly understood. In this work, we report the construction of a chromosomal<jats:italic>shuS<\/jats:italic>mutant. The<jats:italic>shuS<\/jats:italic>mutant was defective in utilizing heme as an iron source. At low heme concentrations, the<jats:italic>shuS<\/jats:italic>mutant grew slowly and its growth was stimulated by either increasing the heme concentration or by providing extra copies of the heme receptor<jats:italic>shuA<\/jats:italic>on a plasmid. At intermediate heme concentrations, the growth of the<jats:italic>shuS<\/jats:italic>mutant was moderately impaired, and at high heme concentrations,<jats:italic>shuS<\/jats:italic>was required for growth on heme. The<jats:italic>shuS<\/jats:italic>mutant did not show increased sensitivity to hydrogen peroxide, even at high heme concentrations. ShuS was also required for optimal utilization of heme under microaerobic and anaerobic conditions. These data are consistent with the model in which ShuS binds heme in a soluble, nontoxic form and potentially transfers the heme from the transport proteins in the membrane to either heme-containing or heme-degrading proteins. ShuS did not appear to store heme for future use.<\/jats:p>","DOI":"10.1128\/jb.187.16.5658-5664.2005","type":"journal-article","created":{"date-parts":[[2005,8,3]],"date-time":"2005-08-03T03:09:11Z","timestamp":1123038551000},"page":"5658-5664","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":58,"title":["<i>Shigella dysenteriae<\/i>ShuS Promotes Utilization of Heme as an Iron Source and Protects against Heme Toxicity"],"prefix":"10.1128","volume":"187","author":[{"given":"Elizabeth E.","family":"Wyckoff","sequence":"first","affiliation":[{"name":"Section of Molecular Genetics and Microbiology and Institute of Cellular and Molecular Biology, The University of Texas, Austin, Texas 78712"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gregory F.","family":"Lopreato","sequence":"additional","affiliation":[{"name":"Section of Molecular Genetics and Microbiology and Institute of Cellular and Molecular Biology, The University of Texas, Austin, Texas 78712"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kimberly A.","family":"Tipton","sequence":"additional","affiliation":[{"name":"Section of Molecular Genetics and Microbiology and Institute of Cellular and Molecular Biology, The University of Texas, Austin, Texas 78712"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shelley M.","family":"Payne","sequence":"additional","affiliation":[{"name":"Section of Molecular Genetics and Microbiology and Institute of Cellular and Molecular Biology, The University of Texas, Austin, Texas 78712"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"9716","DOI":"10.1073\/pnas.0305659101","volume":"101","year":"2004","unstructured":"Alsmark, C. 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