{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T11:53:22Z","timestamp":1773662002713,"version":"3.50.1"},"reference-count":28,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2005,1,15]],"date-time":"2005-01-15T00:00:00Z","timestamp":1105747200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2005,1,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Members of the family<jats:italic>Chlamydiaceae<\/jats:italic>possess at least 13 genes, distributed throughout the chromosome, that are homologous with genes of known type III secretion systems (TTS). The aim of this study was to use putative TTS proteins of<jats:italic>Chlamydophila pneumoniae<\/jats:italic>, whose equivalents in other bacterial TTS function as chaperones, to identify interactions between chlamydial proteins. Using the BacterioMatch Two-Hybrid Vector system (Stratagene, La Jolla, Calif.),<jats:italic>lcrH<\/jats:italic>-<jats:italic>2<\/jats:italic>and<jats:italic>sycE<\/jats:italic>, positions 1021 and 0325, respectively, from<jats:italic>C. pneumoniae<\/jats:italic>CM-1 were used as \u201cbait\u201d to identify target genes (positions 0324, 0705, 0708, 0808 to 0810, 1016 to 1020, and 1022) in close proximity on the chromosome. Interaction between the products of the<jats:italic>lcrH<\/jats:italic>-<jats:italic>2<\/jats:italic>(1021) and<jats:italic>lcrE<\/jats:italic>(<jats:italic>copN<\/jats:italic>) (0324) genes was detected and confirmed by pull-down experiments and enzyme immunoassays using recombinant LcrH-2 and LcrE. As further confirmation of this interaction, the homologous genes from<jats:italic>Chlamydia trachomatis<\/jats:italic>, serovar E, and<jats:italic>Chlamydophila psittaci<\/jats:italic>, Texas turkey, were also cloned in the two-hybrid system to determine if LcrH-2 and LcrE would interact with their orthologs in other species. Consistent with their genetic relatedness, LcrH-2 from<jats:italic>C. pneumoniae<\/jats:italic>interacted with LcrE produced from the three species of<jats:italic>Chlamydiaceae<\/jats:italic>; LcrH-2 from<jats:italic>C. psittaci<\/jats:italic>reacted with LcrE from<jats:italic>C. pneumoniae<\/jats:italic>but not from<jats:italic>C. trachomatis<\/jats:italic>; and<jats:italic>C. trachomatis<\/jats:italic>LcrH-2 did not react with LcrE from the other two species. Deletions from the N and C termini of LcrE from<jats:italic>C. pneumoniae<\/jats:italic>identified the 50 C-terminal amino acids as essential for the interaction with LcrH-2. Thus, it appears that in the<jats:italic>Chlamydiaceae<\/jats:italic>TTS, LcrH-2 interacts with LcrE, and therefore it may serve as a chaperone for this protein.<\/jats:p>","DOI":"10.1128\/jb.187.2.473-479.2005","type":"journal-article","created":{"date-parts":[[2005,1,3]],"date-time":"2005-01-03T18:33:17Z","timestamp":1104777197000},"page":"473-479","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":32,"title":["Interaction between Components of the Type III Secretion System of<i>Chlamydiaceae<\/i>"],"prefix":"10.1128","volume":"187","author":[{"given":"Anatoly","family":"Slepenkin","sequence":"first","affiliation":[{"name":"Department of Pathology, University of California Irvine, Irvine, California"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luis M.","family":"de la Maza","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of California Irvine, Irvine, California"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ellena M.","family":"Peterson","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of California Irvine, Irvine, California"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1099\/00207713-51-1-203","volume":"51","year":"2001","unstructured":"Bush, R. 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