{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T00:10:23Z","timestamp":1791159023812,"version":"4.1.0"},"reference-count":44,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2005,3,15]],"date-time":"2005-03-15T00:00:00Z","timestamp":1110844800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2005,3,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Our laboratory previously constructed mutants of\n            <jats:italic>Mycobacterium tuberculosis<\/jats:italic>\n            and\n            <jats:italic>Mycobacterium smegmatis<\/jats:italic>\n            with deletions in the genes for their major \u03b2-lactamases, BlaC and BlaS, respectively, and showed that the mutants have increased susceptibilities to most \u03b2-lactam antibiotics, particularly the penicillins. However, there is still a basal level of resistance in the mutants to certain penicillins, and the susceptibilities of the mutants to some cephalosporin-based \u03b2-lactams are essentially the same as those of the wild types. We hypothesized that characterizing additional mutants (derived from \u03b2-lactamase deletion mutants) that are hypersusceptible to \u03b2-lactam antibiotics might reveal novel genes involved with other mechanisms of \u03b2-lactam resistance, peptidoglycan assembly, and cell envelope physiology. We report here the isolation and characterization of nine \u03b2-lactam antibiotic-hypersusceptible transposon mutants, two of which have insertions in genes known to be involved with peptidoglycan biosynthesis (\n            <jats:italic>ponA2<\/jats:italic>\n            and\n            <jats:italic>dapB<\/jats:italic>\n            ); the other seven mutants have insertions which affect novel genes. These genes can be classified into three groups: those involved with peptidoglycan biosynthesis, cell division, and other cell envelope processes. Two of the peptidoglycan-biosynthetic genes (\n            <jats:italic>ponA2<\/jats:italic>\n            and\n            <jats:italic>pbpX<\/jats:italic>\n            ) may encode \u03b2-lactam antibiotic-resistant enzymes proposed to be involved with the synthesis of the unusual diaminopimelyl linkages within the mycobacterial peptidoglycan.\n          <\/jats:p>","DOI":"10.1128\/jb.187.6.1892-1900.2005","type":"journal-article","created":{"date-parts":[[2005,3,2]],"date-time":"2005-03-02T18:50:00Z","timestamp":1109789400000},"page":"1892-1900","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":65,"title":["Characterization of Novel\n            <i>Mycobacterium tuberculosis<\/i>\n            and\n            <i>Mycobacterium smegmatis<\/i>\n            Mutants Hypersusceptible to \u03b2-Lactam Antibiotics"],"prefix":"10.1128","volume":"187","author":[{"given":"Anthony R.","family":"Flores","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, Rochester"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linda M.","family":"Parsons","sequence":"additional","affiliation":[{"name":"The Wadsworth Center, New York State Department of Health, Albany, New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr.","given":"Martin S.","family":"Pavelka","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Rochester School of Medicine and Dentistry, Rochester"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.178.1.273-279.1996"},{"key":"e_1_3_2_4_2","doi-asserted-by":"crossref","first-page":"10961","DOI":"10.1073\/pnas.94.20.10961","volume":"94","year":"1997","unstructured":"Bardarov, S., J. 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