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However, to our knowledge, attempts to carry out gene transfer in pathogenic<jats:italic>Leptospira<\/jats:italic>spp. have failed so far. In this study, we show that the<jats:italic>Himar1 mariner<\/jats:italic>transposon permits random mutagenesis in the pathogen<jats:italic>L. interrogans<\/jats:italic>. We have identified genes that have been interrupted by<jats:italic>Himar1<\/jats:italic>insertion in 35<jats:italic>L. interrogans<\/jats:italic>mutants. This approach of transposon mutagenesis will be useful for understanding the spirochetal physiology and the pathogenic mechanisms of<jats:italic>Leptospira<\/jats:italic>, which remain largely unknown.<\/jats:p>","DOI":"10.1128\/jb.187.9.3255-3258.2005","type":"journal-article","created":{"date-parts":[[2005,4,18]],"date-time":"2005-04-18T21:47:59Z","timestamp":1113860879000},"page":"3255-3258","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":97,"title":["Random Insertional Mutagenesis of<i>Leptospira interrogans<\/i>, the Agent of Leptospirosis, Using a<i>mariner<\/i>Transposon"],"prefix":"10.1128","volume":"187","author":[{"given":"Pascale","family":"Bourhy","sequence":"first","affiliation":[{"name":"Laboratoire des Spiroche\u0300tes, Institut Pasteur, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"He\u0301le\u0300ne","family":"Louvel","sequence":"additional","affiliation":[{"name":"Laboratoire des Spiroche\u0300tes, Institut Pasteur, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Saint Girons","sequence":"additional","affiliation":[{"name":"Laboratoire des Spiroche\u0300tes, Institut Pasteur, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathieu","family":"Picardeau","sequence":"additional","affiliation":[{"name":"Laboratoire des Spiroche\u0300tes, Institut Pasteur, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"689","DOI":"10.1099\/mic.0.26065-0","volume":"149","year":"2003","unstructured":"Bauby, H., I. 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