{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T19:03:28Z","timestamp":1789758208928,"version":"4.0.1"},"reference-count":31,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2006,1,15]],"date-time":"2006-01-15T00:00:00Z","timestamp":1137283200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2006,1,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Standard biochemical tests have revealed that hemin and menadione auxotrophic\n            <jats:italic>Staphylococcus aureus<\/jats:italic>\n            small-colony variants (SCVs) exhibit multiple phenotypic changes. To provide a more complete analysis of the SCV phenotype, two genetically defined mutants with a stable SCV phenotype were comprehensively tested. These mutants, generated via mutations in\n            <jats:italic>menD<\/jats:italic>\n            or\n            <jats:italic>hemB<\/jats:italic>\n            that yielded menadione and hemin auxotrophs, were subjected to phenotype microarray (PM) analysis of over 1,500 phenotypes (including utilization of different carbon, nitrogen, phosphate, and sulfur sources; growth stimulation or inhibition by amino acids and other nutrients, osmolytes, and metabolic inhibitors; and susceptibility to antibiotics). Compared to parent strain COL, the\n            <jats:italic>hemB<\/jats:italic>\n            mutant was defective in utilization of a variety of carbon sources, including Krebs cycle intermediates and compounds that ultimately generate ATP via electron transport. The phenotype of the\n            <jats:italic>menD<\/jats:italic>\n            mutant was similar to that of the\n            <jats:italic>hemB<\/jats:italic>\n            mutant, but the defects in carbon metabolism were more pronounced than those seen with the\n            <jats:italic>hemB<\/jats:italic>\n            mutant. In both mutant strains, hexose phosphates and other carbohydrates that provide ATP in the absence of electron transport stimulated growth. Other phenotypes of SCV mutants, such as hypersensitivity to sodium selenite, sodium tellurite, and sodium nitrite, were also uncovered by the PM analysis. Key results of the PM analysis were confirmed in independent growth studies and by using Etest strips for susceptibility testing. PM technology is a new and efficient technology for assessing cellular phenotypes in\n            <jats:italic>S. aureus<\/jats:italic>\n            .\n          <\/jats:p>","DOI":"10.1128\/jb.188.2.687-693.2006","type":"journal-article","created":{"date-parts":[[2005,12,29]],"date-time":"2005-12-29T20:12:50Z","timestamp":1135887170000},"page":"687-693","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":121,"title":["Phenotype Microarray Profiling of\n            <i>Staphylococcus aureus menD<\/i>\n            and\n            <i>hemB<\/i>\n            Mutants with the Small-Colony-Variant Phenotype"],"prefix":"10.1128","volume":"188","author":[{"given":"Christof","family":"von Eiff","sequence":"first","affiliation":[{"name":"Institute of Medical Microbiology, University of Muenster, Muenster, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"McNamara","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology &amp; Immunology; University of Wisconsin Medical School, Madison, Wisconsin"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karsten","family":"Becker","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology, University of Muenster, Muenster, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donna","family":"Bates","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology &amp; Immunology; University of Wisconsin Medical School, Madison, Wisconsin"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang-He","family":"Lei","sequence":"additional","affiliation":[{"name":"Biolog, Inc., Hayward, California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Ziman","sequence":"additional","affiliation":[{"name":"Biolog, Inc., Hayward, California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barry R.","family":"Bochner","sequence":"additional","affiliation":[{"name":"Biolog, Inc., Hayward, California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georg","family":"Peters","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology, University of Muenster, Muenster, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard A.","family":"Proctor","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology &amp; Immunology; University of Wisconsin Medical School, Madison, Wisconsin"},{"name":"Department of Medicine, University of Wisconsin Medical School, Madison, Wisconsin"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"1181","DOI":"10.1099\/00221287-143-4-1181","volume":"143","year":"1997","unstructured":"Avazeri, C., R. 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