{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T17:22:35Z","timestamp":1760980955657},"reference-count":46,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2006,2,1]],"date-time":"2006-02-01T00:00:00Z","timestamp":1138752000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2006,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The\ninner core of neisserial lipooligosaccharide (LOS) contains heptose\nresidues that can be decorated by phosphoethanolamine (PEA). PEA\nmodification of heptose II (HepII) can occur at the 3, 6, or 7\nposition(s). We used a genomic DNA sequence of\n            <jats:italic>lpt3<\/jats:italic>\n            , derived\nfrom\n            <jats:italic>Neisseria meningitidis<\/jats:italic>\n            MC58, to search the genomic\nsequence of\n            <jats:italic>N. gonorrhoeae<\/jats:italic>\n            FA1090 and identified a homolog of\n            <jats:italic>lpt3<\/jats:italic>\n            in\n            <jats:italic>N. gonorrhoeae<\/jats:italic>\n            . A PCR amplicon containing\n            <jats:italic>lpt3<\/jats:italic>\n            was amplified from F62\u0394LgtA, cloned, mutagenized,\nand inserted into the chromosome of\n            <jats:italic>N. gonorrhoeae<\/jats:italic>\n            strain\nF62\u0394LgtA, producing strain\nF62\u0394LgtAlpt3::Tn\n            <jats:italic>5<\/jats:italic>\n            . LOS isolated\nfrom this strain lost the ability to bind monoclonal antibody (MAb)\n2-1-L8. Complementation of this mutation by genetic removal of the\ntransposon insertion restored MAb 2-1-L8 binding. Mass spectrometry\nanalysis of LOS isolated from the F62\u0394LgtA indicated that this\nstrain contained two PEA modifications on its LOS.\nF62\u0394LgtAlpt3::Tn\n            <jats:italic>5<\/jats:italic>\n            lacked a PEA\nmodification on its LOS, a finding consistent with the hypothesis that\n            <jats:italic>lpt3<\/jats:italic>\n            encodes a protein mediating PEA addition onto gonococcal\nLOS. The DNA encoding\n            <jats:italic>lpt3<\/jats:italic>\n            was cloned into an expression\nvector and Lpt3 was purified. Purified Lpt3 was able to mediate the\naddition of PEA to LOS isolated from\nF62\u0394LgtAlpt3::Tn\n            <jats:italic>5<\/jats:italic>\n            .\n          <\/jats:p>","DOI":"10.1128\/jb.188.3.1039-1048.2006","type":"journal-article","created":{"date-parts":[[2006,1,20]],"date-time":"2006-01-20T17:27:29Z","timestamp":1137778049000},"page":"1039-1048","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":16,"title":["Biochemical Analysis of Lpt3, a Protein Responsible forPhosphoethanolamine Addition to Lipooligosaccharide ofPathogenic\n            <i>Neisseria<\/i>"],"prefix":"10.1128","volume":"188","author":[{"given":"Ellen T.","family":"O'Connor","sequence":"first","affiliation":[{"name":"Department\rof Cell Biology and Molecular Genetics, University of Maryland, College\rPark, Maryland 20742"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrzej","family":"Piekarowicz","sequence":"additional","affiliation":[{"name":"Institute of Microbiology,\rUniversity of Warsaw, 02-096 Warsaw, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karen V.","family":"Swanson","sequence":"additional","affiliation":[{"name":"Department\rof Cell Biology and Molecular Genetics, University of Maryland, College\rPark, Maryland 20742"},{"name":"Department of Laboratory\rMedicine and Veterans Affairs Medical Center, University of\rCalifornia San Francisco, San Francisco, California\r94121"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. McLeod","family":"Griffiss","sequence":"additional","affiliation":[{"name":"Department of Laboratory\rMedicine and Veterans Affairs Medical Center, University of\rCalifornia San Francisco, San Francisco, California\r94121"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel C.","family":"Stein","sequence":"additional","affiliation":[{"name":"Department\rof Cell Biology and Molecular Genetics, University of Maryland, College\rPark, Maryland 20742"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"10872","DOI":"10.1073\/pnas.95.18.10872","volume":"95","year":"1998","unstructured":"Banerjee, A., R. Wang, P. A. Rice, E. C. Gotschlich, and D. C. Stein. 1998. Identification of the gene (lgtG) encoding the lipooligosaccharide \u03b2 chain synthesizing glucosyl transferase from Neisseria gonorrhoeae.Proc. Natl. Acad. Sci. USA95:10872-10877.","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"1513","DOI":"10.1093\/nar\/7.6.1513","volume":"7","year":"1979","unstructured":"Birnboim, H. C., and J. Doly. 1979. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.Nucleic Acids Res.7:1513-1523.","journal-title":"Nucleic Acids Res."},{"key":"e_1_3_2_4_2","doi-asserted-by":"crossref","first-page":"1061","DOI":"10.1093\/glycob\/9.10.1061","volume":"9","year":"1999","unstructured":"Blixt, O., I. van Die, T. Norberg, and D. H. van den Eijnden. 1999 . High-level expression of the Neisseria meningitidis lgtA gene in Escherichia coli and characterization of the encoded N-acetylglucosaminyltransferase as a useful catalyst in the synthesis of GlcNAc \u03b21\u21923Gal and GalNAc\u03b2 1\u21923Gal linkages. Glycobiology9:1061-1071.","journal-title":"Glycobiology"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.179.3.982-986.1997"},{"key":"e_1_3_2_6_2","doi-asserted-by":"crossref","first-page":"1435","DOI":"10.1016\/S0008-6215(02)00161-1","volume":"337","year":"2002","unstructured":"Cox, A. D., J. Li, J. R. Brisson, E. R. Moxon, and J. C. Richards. 2002. Structural analysis of the lipopolysaccharide from Neisseria meningitidis strain BZ157 galE: localization of two phosphoethanolamine residues in the inner core oligosaccharide. Carbohydr. Res.337:1435-1444.","journal-title":"Carbohydr. Res."},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.177.24.7275-7279.1995"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.177.9.2321-2327.1995"},{"key":"e_1_3_2_9_2","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1073\/pnas.86.1.17","volume":"86","year":"1989","unstructured":"Gibson, B. W., J. W. Webb, R. Yamasaki, S. J. Fisher, A. L. Burlingame, R. E. Mandrell, H. Schneider, and J. M. Griffiss. 1989. Structure and heterogeneity of the oligosaccharides from the lipopolysaccharides of a pyocin-resistant Neisseria gonorrhoeae. Proc. Natl. Acad. Sci. USA86:17-21.","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"e_1_3_2_10_2","doi-asserted-by":"crossref","first-page":"7367","DOI":"10.1074\/jbc.273.13.7367","volume":"273","year":"1998","unstructured":"Goryshin, I. Y., and W. S. Reznikoff. 1998. Tn5 in vitro transposition. J. Biol. Chem.273:7367-7374.","journal-title":"J. Biol. Chem."},{"key":"e_1_3_2_11_2","doi-asserted-by":"crossref","first-page":"2181","DOI":"10.1084\/jem.180.6.2181","volume":"180","year":"1994","unstructured":"Gotschlich, E. C. 1994. Genetic locus for the biosynthesis of the variable portion of Neisseria gonorrhoeae lipooligosaccharide. J. Exp. Med.180:2181-2190.","journal-title":"J. Exp. Med."},{"key":"e_1_3_2_12_2","doi-asserted-by":"crossref","first-page":"9716","DOI":"10.1074\/jbc.275.13.9716","volume":"275","year":"2000","unstructured":"Griffiss, J. M., B. L. Brandt, N. B. Saunders, and W. Zollinger. 2000. Structural relationships and sialylation among meningococcal L1, L8 and L3,7 lipooligosaccharide serotypes. J. Biol. Chem.275:9716-9724.","journal-title":"J. Biol. Chem."},{"key":"e_1_3_2_13_2","first-page":"509","volume":"251","year":"1996","unstructured":"Gunn, J. S., and D. C. Stein. 1996. Use of a non-selectable transformation technique to construct a multiple restriction modification deficient mutant of Neisseria gonorrhoeae. Mol. Gen. Genet.251:509-517.","journal-title":"Mol. Gen. Genet."},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.154.1.269-277.1983"},{"key":"e_1_3_2_15_2","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/0378-1119(90)90336-P","volume":"96","year":"1990","unstructured":"Inoue, H., H. Nojima, and H. Okayama. 1990. High efficiency transformation of Escherichia coli with plasmids.Gene96:23-28.","journal-title":"Gene"},{"key":"e_1_3_2_16_2","doi-asserted-by":"crossref","first-page":"19303","DOI":"10.1016\/S0021-9258(18)54998-X","volume":"266","year":"1991","unstructured":"John, C. M., J. M. Griffiss, M. A. Apicella, R. E. Mandrell, and B. W. Gibson. 1991 . The structural basis for pyocin resistance in Neisseria gonorrhoeae lipooligosaccharides.J. Biol. Chem.266:19303-19311.","journal-title":"J. Biol. Chem."},{"key":"e_1_3_2_17_2","doi-asserted-by":"crossref","first-page":"1017","DOI":"10.1074\/jbc.274.2.1017","volume":"274","year":"1999","unstructured":"John, C. M., H. Schneider, and J. M. Griffiss. 1999 . Neisseria gonorrhoeae that infect men have lipooligosaccharides with terminal N-acetyl-lactosamine repeats. J. Biol. Chem.274:1017-1025.","journal-title":"J. Biol. Chem."},{"key":"e_1_3_2_18_2","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/0882-4010(92)90022-G","volume":"13","year":"1992","unstructured":"Jones, D. M., R. Borrow, A. J. Fox, S. Gray, K. A. Cartwright, and J. T. Poolman. 1992. The lipooligosaccharide immunotype as a virulence determinant in Neisseria meningitidis. Microb. Pathog.13:219-224.","journal-title":"Microb. Pathog."},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.178.23.6677-6684.1996"},{"key":"e_1_3_2_20_2","first-page":"409","volume":"15","year":"2004","unstructured":"Kahler, C. M., A. Datta, Y. L. Tzeng, R. Carlson, and D. Stephens. 2004. Inner core assembly and structure of the lipooligosaccharide of Neisseria meningitidis: capacity of strain NMB to express all known immunotype epitopes.Glycobiology15:409-419.","journal-title":"Glycobiology"},{"key":"e_1_3_2_21_2","doi-asserted-by":"crossref","first-page":"1156","DOI":"10.1074\/jbc.M009019200","volume":"276","year":"2001","unstructured":"Kanipes, M. I., S. Lin, R. J. Cotter, and C. R. Raetz. 2001 . Ca2+-induced phosphoethanolamine transfer to the outer 3-deoxy-d-manno-octulosonic acid moiety of Escherichia coli lipopolysaccharide: a novel membrane enzyme dependent upon phosphatidylethanolamine.J. Biol. Chem.276:1156-1163.","journal-title":"J. Biol. Chem."},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.13.4124-4133.2004"},{"key":"e_1_3_2_23_2","doi-asserted-by":"crossref","first-page":"931","DOI":"10.1046\/j.1365-2958.2002.02754.x","volume":"43","year":"2002","unstructured":"Mackinnon, F. G., A. D. Cox, J. S. Plested, C. M. Tang, K. Makepeace, P. A. Coull, J. C. Wright, R. Chalmers, D. W. Hood, J. C. Richards, and E. R. Moxon. 2002. Identification of a gene (lpt-3) required for the addition of phosphoethanolamine to the lipopolysaccharide inner core of Neisseria meningitidis and its role in mediating susceptibility to bactericidal killing and opsonophagocytosis. Mol. Microbiol.43:931-943.","journal-title":"Mol. Microbiol."},{"key":"e_1_3_2_24_2","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1093\/glycob\/9.2.157","volume":"9","year":"1999","unstructured":"M\u00fchlecker, W., S. Gulati, D. P. McQuillen, S. Ram, P. A. Rice, and V. N. Reinhold. 1999. An essential saccharide binding domain for the MAb 2C7 established for Neisseria gonorrhoeae LOS by ES-MS and MSn. Glycobiology9:157-171.","journal-title":"Glycobiology"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.184.23.6410-6416.2002"},{"key":"e_1_3_2_26_2","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/S0022-1759(00)00142-3","volume":"237","year":"2000","unstructured":"Plested, J. S., M. A. Gidney, P. A. Coull, H. G. Griffiths, M. A. Herbert, A. G. Bird, J. C. Richards, and E. R. Moxon. 2000 . Enzyme linked immunosorbent assay (ELISA) for the detection of serum antibodies to the inner core lipopolysaccharide of Neisseria meningitidis group B. J. Immunol. Methods237:73-84.","journal-title":"J. Immunol. Methods"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.10.5417-5426.1999"},{"key":"e_1_3_2_28_2","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1006\/mpat.1997.0191","volume":"24","year":"1998","unstructured":"Rahman, M., A. B. Jonsson, and T. Holme. 1998. Monoclonal antibodies to the epitope\u03b1 -Gal-(1-4)-\u03b2-Gal-(1- of Moraxella catarrhalis LPS react with a similar epitope in type IV pili of Neisseria meningitidis. Microb. Pathog.24:299-308.","journal-title":"Microb. Pathog."},{"key":"e_1_3_2_29_2","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1016\/S0008-6215(98)00012-3","volume":"307","year":"1998","unstructured":"Rahman, M. M., D. S. Stephens, C. M. Kahler, J. Glushka, and R. W. Carlson. 1998. The lipooligosaccharide (LOS) of Neisseria meningitidis serogroup B strain NMB contains L2, L3, and novel oligosaccharides, and lacks the lipid-A 4\u2032-phosphate substituent. Carbohydr. Res.307:311-324.","journal-title":"Carbohydr. Res."},{"key":"e_1_3_2_30_2","first-page":"50851","volume":"278","year":"2003","unstructured":"Ram, S., A. D. Cox, J. C. Wright, U. Vogel, S. Getzlaff, R. Boden, J. Li, J. S. Plested, S. Meri, S. Gulati, D. C. Stein, J. C. Richards, E. R. Moxon, and P. A. Rice. 2003. Neisserial lipooligosaccharide is a target for complement component C4b: inner core phosphoethanolamine residues define C4b linkage specificity.J. Biol. Chem.278:50851-50862.","journal-title":"J. Biol. Chem."},{"key":"e_1_3_2_31_2","doi-asserted-by":"crossref","first-page":"21202","DOI":"10.1074\/jbc.M500964200","volume":"280","year":"2005","unstructured":"Reynolds, C. M., S. R. Kalb, R. J. Cotter, and C. R. Raetz. 2005. A phosphoethanolamine transferase specific for the outer 3-deoxy-d-manno-octulosonic acid residue of Escherichia coli lipopolysaccharide. Identification of the eptB gene and Ca2+ hypersensitivity of an eptB deletion mutant. J. Biol. Chem.280:21202-21211.","journal-title":"J. Biol. Chem."},{"key":"e_1_3_2_32_2","first-page":"83","volume":"260","year":"2004","unstructured":"Reznikoff, W. S., I. Y. Goryshin, and J. J. Jendrisak. 2004. Tn5 as a molecular genetics tool: in vitro transposition and the coupling of in vitro technologies with in vivo transposition. Methods Mol. Biol.260:83-96.","journal-title":"Methods Mol. Biol."},{"key":"e_1_3_2_33_2","unstructured":"Sambrook J. E. F. Fritsch and T. Maniatis. 1989.\nMolecular cloning: a laboratory manual 2nd ed. Cold Spring Harbor\nLaboratory Press Cold Spring Harbor N.Y."},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.176.22.6869-6876.1994"},{"key":"e_1_3_2_35_2","doi-asserted-by":"crossref","first-page":"949","DOI":"10.1084\/jem.191.6.949","volume":"191","year":"2000","unstructured":"Song, W., L. Ma, W. Chen, and D. C. Stein. 2000. Role of lipooligosaccharide in opa-independent invasion of Neisseria gonorrhoeae into human epithelial cells. J. Exp. Med.191:949-959.","journal-title":"J. Exp. Med."},{"key":"e_1_3_2_36_2","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1139\/m91-056","volume":"37","year":"1991","unstructured":"Stein, D. C. 1991. Transformation of Neisseria gonorrhoeae: physical requirements of the transforming DNA.Can. J. Microbiol.37:345-349.","journal-title":"Can. J. Microbiol."},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.3.942-950.2001"},{"key":"e_1_3_2_38_2","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/0003-2697(82)90673-X","volume":"119","year":"1982","unstructured":"Tsai, C. M., and C. E. Frasch. 1982. A sensitive silver stain for detecting lipooligosaccharide in polyacrylamide gels. Anal. Biochem.119:115-119.","journal-title":"Anal. Biochem."},{"key":"e_1_3_2_39_2","doi-asserted-by":"crossref","first-page":"1007","DOI":"10.1016\/j.carres.2003.11.014","volume":"339","year":"2004","unstructured":"Tvaroska, I. 2004. Molecular modeling insights into the catalytic mechanism of the retaining galactosyltransferase LgtC.Carbohydr. Res.339:1007-1014.","journal-title":"Carbohydr. Res."},{"key":"e_1_3_2_40_2","first-page":"35053","year":"2004","unstructured":"Tzeng, Y. L., A. Datta, K. Ambrose, M. Lo, J. K. Davies, R. W. Carlson, D. S. Stephens, and C. M. Kahler. 2004. The MisR\/MisS two-component regulatory system influences inner core structure and immunotype of lipooligosaccharide in Neisseria meningitidis.J. Biol. Chem.279:35053-35062.","journal-title":"J. Biol. Chem."},{"key":"e_1_3_2_41_2","doi-asserted-by":"crossref","first-page":"19166","DOI":"10.1074\/jbc.271.32.19166","volume":"271","year":"1996","unstructured":"Wakarchuk, W., A. Martin, M. P. Jennings, E. R. Moxon, and J. C. Richards. 1996. Functional relationships of the genetic locus encoding the glycosyltransferase enzymes involved in expression of the lacto-N-neotetraose terminal lipopolysaccharide structure in Neisseria meningitidis. J. Biol. Chem.271:19166-19173.","journal-title":"J. Biol. Chem."},{"key":"e_1_3_2_42_2","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1093\/glycob\/cwh074","volume":"14","year":"2004","unstructured":"Wakarchuk, W., M. J. Schur, F. St Michael, J. Li, E. Eichler, and D. Whitfield. 2004. Characterization of the\u03b1 -1,2-N-acetylglucosaminyltransferase of Neisseria gonorrhoeae, a key control point in lipooligosaccharide biosynthesis. Glycobiology14:537-546.","journal-title":"Glycobiology"},{"key":"e_1_3_2_43_2","first-page":"83","volume":"5","year":"1965","unstructured":"Westphal, O., and K. Jann. 1965. Bacterial lipopolysaccharides: extraction with phenol-water and further applications of the procedure. Methods Carbohydr. Chem.5:83-91.","journal-title":"Methods Carbohydr. Chem."},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/am.13.2.171-174.1965"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.20.6970-6982.2004"},{"key":"e_1_3_2_46_2","doi-asserted-by":"crossref","first-page":"30345","DOI":"10.1016\/S0021-9258(18)43819-7","volume":"269","year":"1994","unstructured":"Yamasaki, R., D. E. Kerwood, H. Schneider, K. P. Quinn, J. M. Griffiss, and R. E. Mandrell. 1994 . The structure of lipooligosaccharide produced by Neisseria gonorrhoeae, strain 15253, isolated from a patient with disseminated infection: evidence for a new glycosylation pathway of the gonococcal lipooligosaccharide. J. Biol. Chem.269:30345-30351.","journal-title":"J. Biol. Chem."},{"key":"e_1_3_2_47_2","doi-asserted-by":"crossref","first-page":"1233","DOI":"10.1016\/0161-5890(91)90010-H","volume":"28","year":"1991","unstructured":"Yamasaki, R., W. Nasholds, H. Schneider, and M. A. Apicella. 1991 . Epitope expression and partial structural characterization of F62 lipooligosaccharide (LOS) of Neisseria gonorrhoeae: IgM monoclonal antibodies (3F11 and 1-1-M) recognize non-reducing termini of the LOS components. Mol. Immunol.28:1233-1242.","journal-title":"Mol. Immunol."}],"container-title":["Journal of Bacteriology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JB.188.3.1039-1048.2006","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JB.188.3.1039-1048.2006","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T17:31:39Z","timestamp":1627579899000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JB.188.3.1039-1048.2006"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,2]]},"references-count":46,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2006,2]]}},"alternative-id":["10.1128\/JB.188.3.1039-1048.2006"],"URL":"https:\/\/doi.org\/10.1128\/jb.188.3.1039-1048.2006","relation":{},"ISSN":["0021-9193","1098-5530"],"issn-type":[{"value":"0021-9193","type":"print"},{"value":"1098-5530","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,2]]},"assertion":[{"value":"2005-04-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2005-10-18","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-20","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}