{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T17:13:37Z","timestamp":1783617217977,"version":"3.55.0"},"reference-count":31,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2006,2,1]],"date-time":"2006-02-01T00:00:00Z","timestamp":1138752000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2006,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            In\n            <jats:italic>Escherichia coli<\/jats:italic>\n            , nitrosative mutagenesis may occur during nitrate or nitrite respiration. The endogenous nitrosating agent N\n            <jats:sub>2<\/jats:sub>\n            O\n            <jats:sub>3<\/jats:sub>\n            (dinitrogen trioxide, nitrous anhydride) may be formed either by the condensation of nitrous acid or by the autooxidation of nitric oxide, both of which are metabolic by-products. The purpose of this study was to determine which of these two agents is more responsible for endogenous nitrosative mutagenesis. An\n            <jats:italic>nfi<\/jats:italic>\n            (endonuclease V) mutant was grown anaerobically with nitrate or nitrite, conditions under which it has a high frequency of A:T-to-G:C transition mutations because of a defect in the repair of hypoxanthine (nitrosatively deaminated adenine) in DNA. These mutations could be greatly reduced by two means: (i) introduction of an\n            <jats:italic>nirB<\/jats:italic>\n            mutation, which affects the inducible cytoplasmic nitrite reductase, the major source of nitric oxide during nitrate or nitrite metabolism, or (ii) flushing the anaerobic culture with argon (which should purge it of nitric oxide) before it was exposed to air. The results suggest that nitrosative mutagenesis occurs during a shift from nitrate\/nitrite-dependent respiration under hypoxic conditions to aerobic respiration, when accumulated nitric oxide reacts with oxygen to form endogenous nitrosating agents such as N\n            <jats:sub>2<\/jats:sub>\n            O\n            <jats:sub>3<\/jats:sub>\n            . In contrast, mutagenesis of nongrowing cells by nitrous acid was unaffected by an\n            <jats:italic>nirB<\/jats:italic>\n            mutation, suggesting that this mutagenesis is mediated by N\n            <jats:sub>2<\/jats:sub>\n            O\n            <jats:sub>3<\/jats:sub>\n            that is formed directly by the condensation of nitrous acid.\n          <\/jats:p>","DOI":"10.1128\/jb.188.3.829-833.2006","type":"journal-article","created":{"date-parts":[[2006,1,20]],"date-time":"2006-01-20T17:27:29Z","timestamp":1137778049000},"page":"829-833","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":60,"title":["Evidence for Mutagenesis by Nitric Oxide during Nitrate Metabolism in\n            <i>Escherichia coli<\/i>"],"prefix":"10.1128","volume":"188","author":[{"given":"Bernard","family":"Weiss","sequence":"first","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia 30322"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"1223","DOI":"10.1093\/nar\/27.5.1223","volume":"27","year":"1999","unstructured":"Aravind, L., D. 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