{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T02:45:41Z","timestamp":1793155541916,"version":"4.5.0"},"reference-count":24,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2006,3,1]],"date-time":"2006-03-01T00:00:00Z","timestamp":1141171200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2006,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The\n            <jats:italic>Brucella<\/jats:italic>\n            cell envelope is characterized by the presence of phosphatidylcholine (PC), a common phospholipid in eukaryotes that is rare in prokaryotes. Studies on the composition of\n            <jats:italic>Brucella abortus<\/jats:italic>\n            2308 phospholipids revealed that the synthesis of PC depends on the presence of choline in the culture medium, suggesting that the methylation biosynthetic pathway is not functional. Phospholipid composition of\n            <jats:italic>pmtA<\/jats:italic>\n            and\n            <jats:italic>pcs<\/jats:italic>\n            mutants indicated that in\n            <jats:italic>Brucella<\/jats:italic>\n            , PC synthesis occurs exclusively via the phosphatidylcholine synthase pathway. Transformation of\n            <jats:italic>Escherichia coli<\/jats:italic>\n            with an expression vector containing the\n            <jats:italic>B. abortus pcs<\/jats:italic>\n            homologue was sufficient for PC synthesis upon induction with IPTG (isopropyl-\u03b2-\n            <jats:sc>d<\/jats:sc>\n            -thiogalactopyranoside), while no PC formation was detected when bacteria were transformed with a vector containing\n            <jats:italic>pmtA.<\/jats:italic>\n            These findings imply that\n            <jats:italic>Brucella<\/jats:italic>\n            depends on choline provided by the host cell to form PC. We could not detect any obvious associated phenotype in the PC-deficient strain under vegetative or intracellular growth conditions in macrophages. However, the\n            <jats:italic>pcs<\/jats:italic>\n            mutant strain displays a reproducible virulence defect in mice, which suggests that PC is necessary to sustain a chronic infection process.\n          <\/jats:p>","DOI":"10.1128\/jb.188.5.1929-1934.2006","type":"journal-article","created":{"date-parts":[[2006,2,16]],"date-time":"2006-02-16T02:38:02Z","timestamp":1140057482000},"page":"1929-1934","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":90,"title":["<i>Brucella abortus<\/i>\n            Synthesizes Phosphatidylcholine from Choline Provided by the Host"],"prefix":"10.1128","volume":"188","author":[{"given":"Diego J.","family":"Comerci","sequence":"first","affiliation":[{"name":"Instituto de Investigaciones Biotecnolo\u0301gicas, Universidad Nacional de General San Marti\u0301n, CONICET, San Marti\u0301n 1650, Buenos Aires, Argentina"},{"name":"Comisio\u0301n Nacional de Energi\u0301a Ato\u0301mica, Divisio\u0301n Agropecuaria, Centro Ato\u0301mico Ezeiza 1804, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Altabe","sequence":"additional","affiliation":[{"name":"Instituto de Biologi\u0301a Molecular y Celular de Rosario (IBR) and Departamento de Microbiologi\u0301a, Facultad de Ciencias Bioqui\u0301micas y Farmace\u0301uticas, Universidad Nacional de Rosario, Suipacha 531, S2002LRK Rosario, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diego","family":"de Mendoza","sequence":"additional","affiliation":[{"name":"Instituto de Biologi\u0301a Molecular y Celular de Rosario (IBR) and Departamento de Microbiologi\u0301a, Facultad de Ciencias Bioqui\u0301micas y Farmace\u0301uticas, Universidad Nacional de Rosario, Suipacha 531, S2002LRK Rosario, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodolfo A.","family":"Ugalde","sequence":"additional","affiliation":[{"name":"Comisio\u0301n Nacional de Energi\u0301a Ato\u0301mica, Divisio\u0301n Agropecuaria, Centro Ato\u0301mico Ezeiza 1804, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1139\/o59-099"},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","first-page":"965","DOI":"10.1099\/0022-1317-50-11-965","volume":"50","year":"2001","unstructured":"Casao, M. 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