{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T06:13:37Z","timestamp":1791094417816,"version":"4.1.0"},"reference-count":44,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2006,3,15]],"date-time":"2006-03-15T00:00:00Z","timestamp":1142380800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2006,3,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Group B streptococcus (GBS) remains a major cause of morbidity and mortality among newborn children. The bacterium is a commensal organism colonizing the rectum and the gastrointestinal and urogenital tracts of adults, but it can be transmitted to neonates by an ascending infection of the maternal genital tract or during parturition. We previously reported that a transposon insertion disrupting\n                    <jats:italic>rpoE<\/jats:italic>\n                    resulted in the decreased survival of the mutant in the neonatal rat sepsis model of GBS infection.\n                    <jats:italic>rpoE<\/jats:italic>\n                    encodes the \u03b4 protein, a subunit of RNA polymerase (RNAP) that has been characterized in\n                    <jats:italic>Bacillus<\/jats:italic>\n                    species. In this study, we confirm the association of the \u03b4 protein with purified GBS RNAP and show that it is expressed in strains representing all nine serotypes. Flow cytometric analysis of a reporter strain containing a transcriptional fusion of the\n                    <jats:italic>rpoE<\/jats:italic>\n                    promoter to\n                    <jats:italic>gfp<\/jats:italic>\n                    revealed that, in vitro, this gene is continuously expressed. Analysis of \u03b4 expression in the transposon mutant by quantitative Western blotting revealed a 10-fold reduction in relative abundance (which was linked to the attenuation in virulence that was observed for this mutant) compared to that for the wild-type strain. These data suggest that a minimum intracellular concentration of \u03b4 is necessary for this organism to cause disease.\n                  <\/jats:p>","DOI":"10.1128\/jb.188.6.2096-2105.2006","type":"journal-article","created":{"date-parts":[[2006,3,2]],"date-time":"2006-03-02T09:22:50Z","timestamp":1141291370000},"page":"2096-2105","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":30,"title":["Abundance of the \u03b4 Subunit of RNA Polymerase Is Linked to the Virulence of\n                    <i>Streptococcus agalactiae<\/i>"],"prefix":"10.1128","volume":"188","author":[{"given":"Ravin","family":"Seepersaud","sequence":"first","affiliation":[{"name":"Department of Pediatrics, Division of Infectious Disease, Children's Hospital and Regional Medical Center and University of Washington, Seattle, Washington, 98109"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rachel H. V.","family":"Needham","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Division of Infectious Disease, Children's Hospital and Regional Medical Center and University of Washington, Seattle, Washington, 98109"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cathy S.","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Division of Infectious Disease, Children's Hospital and Regional Medical Center and University of Washington, Seattle, Washington, 98109"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda L.","family":"Jones","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Division of Infectious Disease, Children's Hospital and Regional Medical Center and University of Washington, Seattle, Washington, 98109"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"2893","DOI":"10.1093\/nar\/10.9.2893","volume":"10","year":"1982","unstructured":"Achberger, E. 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