{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T15:41:09Z","timestamp":1762270869796},"reference-count":59,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2006,3,15]],"date-time":"2006-03-15T00:00:00Z","timestamp":1142380800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[2006,3,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The flagella of the soil bacterium\n            <jats:italic>Sinorhizobium meliloti<\/jats:italic>\n            differ from the enterobacterial paradigm in the complex filament structure and modulation of the flagellar rotary speed. The mode of motility control in\n            <jats:italic>S. meliloti<\/jats:italic>\n            has a molecular corollary in two novel periplasmic motility proteins, MotC and MotE, that are present in addition to the ubiquitous MotA\/MotB energizing proton channel. A fifth motility gene is located in the\n            <jats:italic>mot<\/jats:italic>\n            operon downstream of the\n            <jats:italic>motB<\/jats:italic>\n            and\n            <jats:italic>motC<\/jats:italic>\n            genes. Its gene product was originally designated MotD, a cytoplasmic motility protein having an unknown function. We report here reassignment of MotD as FliK, the regulator of flagellar hook length. The FliK gene is one of the few flagellar genes not annotated in the contiguous flagellar regulon of\n            <jats:italic>S. meliloti<\/jats:italic>\n            . Characteristic for its class, the 475-residue FliK protein contains a conserved, compactly folded Flg hook domain in its carboxy-terminal region. Deletion of\n            <jats:italic>fliK<\/jats:italic>\n            leads to formation of prolonged flagellar hooks (polyhooks) with missing filament structures. Extragenic suppressor mutations all mapped in the cytoplasmic region of the transmembrane export protein FlhB and restored assembly of a flagellar filament, and thus motility, in the presence of polyhooks. The structural properties of FliK are consistent with its function as a substrate specificity switch of the flagellar export apparatus for switching from rod\/hook-type substrates to filament-type substrates.\n          <\/jats:p>","DOI":"10.1128\/jb.188.6.2144-2153.2006","type":"journal-article","created":{"date-parts":[[2006,3,2]],"date-time":"2006-03-02T14:22:50Z","timestamp":1141309370000},"page":"2144-2153","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":25,"title":["MotD of\n            <i>Sinorhizobium meliloti<\/i>\n            and Related \u03b1-Proteobacteria Is the Flagellar-Hook-Length Regulator and Therefore Reassigned as FliK"],"prefix":"10.1128","volume":"188","author":[{"given":"Elke","family":"Eggenhofer","sequence":"first","affiliation":[{"name":"Institut fu\u0308r Biochemie, Genetik und Mikrobiologie, Universita\u0308t Regensburg, D-93040 Regensburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reinhard","family":"Rachel","sequence":"additional","affiliation":[{"name":"Institut fu\u0308r Biochemie, Genetik und Mikrobiologie, Universita\u0308t Regensburg, D-93040 Regensburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin","family":"Haslbeck","sequence":"additional","affiliation":[{"name":"Department Chemie, Technische Universita\u0308t Mu\u0308nchen, D-85747 Garching, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Birgit","family":"Scharf","sequence":"additional","affiliation":[{"name":"Institut fu\u0308r Biochemie, Genetik und Mikrobiologie, Universita\u0308t Regensburg, D-93040 Regensburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1046\/j.1365-2958.1996.344874.x","volume":"19","year":"1996","unstructured":"Aizawa, S. 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