{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,4,4]],"date-time":"2022-04-04T12:56:29Z","timestamp":1649076989060},"reference-count":6,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[1962,1,1]],"date-time":"1962-01-01T00:00:00Z","timestamp":-252460800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[1962,1]]},"abstract":"<jats:p>\n            <jats:sc>Pan, Sylvia F.<\/jats:sc>\n            (University of Pittsburgh, Pittsburgh, Pa.),\n            <jats:sc>Robert B. Yee, and Horace M. Gezon<\/jats:sc>\n            . Studies on the metabolism of\n            <jats:italic>Shigella<\/jats:italic>\n            . IV. Nutritional requirements of\n            <jats:italic>Shigella flexneri<\/jats:italic>\n            serotype 3. J. Bacteriol.\n            <jats:bold>83:<\/jats:bold>\n            61\u201365. 1962.\u2014A study of the nutritional requirements of five strains of\n            <jats:italic>Shigella flexneri<\/jats:italic>\n            serotype 3 was carried out. Four of the strains could be cultured in a synthetic medium composed of a mineral salts glucose base, NH\n            <jats:sub>4<\/jats:sub>\n            H\n            <jats:sub>2<\/jats:sub>\n            PO\n            <jats:sub>4<\/jats:sub>\n            or an amino acid as the nitrogen source, and nicotinamide; the fifth one required the presence of both NH\n            <jats:sub>4<\/jats:sub>\n            H\n            <jats:sub>2<\/jats:sub>\n            PO\n            <jats:sub>4<\/jats:sub>\n            and an amino acid in the medium. Variation among the former strains in their nitrogen and vitamin requirements was observed. The least fastidious one produced maximal growth using the simple ammonium salt as its source of nitrogen and did not require the addition of nicotinamide. Two strains were moderately fastidious, producing better growth within 24 hr in the presence of\n            <jats:sc>l<\/jats:sc>\n            -aspartic acid instead of NH\n            <jats:sub>4<\/jats:sub>\n            H\n            <jats:sub>2<\/jats:sub>\n            PO\n            <jats:sub>4<\/jats:sub>\n            , and requiring nicotinamide as an accessory growth factor. The fourth strain was extremely fastidious, growing poorly or not at all in the synthetic medium. Incubation of large inocula of the fastidious strain in synthetic medium and over a prolonged period resulted in adaptation of the organism to the medium. Some of the adapted cultures grew readily in the presence of NH\n            <jats:sub>4<\/jats:sub>\n            H\n            <jats:sub>2<\/jats:sub>\n            PO\n            <jats:sub>4<\/jats:sub>\n            and did not require nicotinamide.\n          <\/jats:p>","DOI":"10.1128\/jb.83.1.61-65.1962","type":"journal-article","created":{"date-parts":[[2020,1,3]],"date-time":"2020-01-03T21:21:08Z","timestamp":1578086468000},"page":"61-65","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["STUDIES ON THE METABOLISM OF\n            <i>SHIGELLA<\/i>\n            IV.  3"],"prefix":"10.1128","volume":"83","author":[{"given":"Sylvia F.","family":"Pan","sequence":"first","affiliation":[{"name":"Graduate School of Public Health, University of Pittsburgh, Pittsburgh, Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert B.","family":"Yee","sequence":"additional","affiliation":[{"name":"Graduate School of Public Health, University of Pittsburgh, Pittsburgh, Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Horace M.","family":"Gezon","sequence":"additional","affiliation":[{"name":"Graduate School of Public Health, University of Pittsburgh, Pittsburgh, Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"p_1","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1093\/infdis\/65.2.163","article-title":"Nicotinamide and related compounds as essential growth substances for dysentery bacilli","volume":"65","author":"DORFMAN A., S.","year":"1939","unstructured":"DORFMAN , A., S. A. KOSER , H. R. REAMES , K. F. SWINGLE , A ND F . SAUNDERS. 1939 . Nicotinamide and related compounds as essential growth substances for dysentery bacilli . J. Infectious Diseases 65 : 163 - 182 .","journal-title":"J. Infectious Diseases"},{"key":"p_2","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1128\/jb.75.3.253-257.1958","article-title":"Nutrition of Shigella: growth of Shigella flexneri 3 in a simple chemically defined medium","volume":"75","author":"ERLANDSON A. L.","year":"1958","unstructured":"ERLANDSON , A. L. , JR ., AND W. H. MACKEY. 1958 . Nutrition of Shigella: growth of Shigella flexneri 3 in a simple chemically defined medium . J. Bacteriol. 75 : 253 - 257 .","journal-title":"J. Bacteriol."},{"key":"p_3","unstructured":"GYORGY P. 1951. Vitamin methods vol. 2. Academic Press Inc. New York."},{"key":"p_4","doi-asserted-by":"crossref","first-page":"311","DOI":"10.3181\/00379727-38-9832P","article-title":"Nicotinic acid as an essential growthsubstance for dysentery bacilli","volume":"38","author":"SER S. A.","year":"1938","unstructured":"Ko SER , S. A. , A. DORFMAN , A ND F . SAUNDERS. 1938 . Nicotinic acid as an essential growthsubstance for dysentery bacilli . Proc. Soc. Exptl. Biol. Med. 38 : 311 - 313 .","journal-title":"Proc. Soc. Exptl. Biol. Med."},{"key":"p_5","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1128\/jb.63.3.399-406.1952","article-title":"Replica plating and indirect selection of bacterial mutants","volume":"63","author":"LEDERBERG J.","year":"1952","unstructured":"LEDERBERG , J. , AND E. M. LEDERBERG. 1952 . Replica plating and indirect selection of bacterial mutants . J. Bacteriol. 63 : 399 - 406 .","journal-title":"J. Bacteriol."},{"key":"p_6","doi-asserted-by":"crossref","first-page":"24","DOI":"10.3181\/00379727-55-14442","article-title":"Nicotinic acid and pantothenic acid as essential growth factors for Shigella paradysenteriae (Flexner)","volume":"55","author":"WEIL A. J.","year":"1944","unstructured":"WEIL , A. J. , AND J. BLACK. 1944 . Nicotinic acid and pantothenic acid as essential growth factors for Shigella paradysenteriae (Flexner) . Proc. Soc. Exptl. Biol. Med. 55 : 24 - 26 .","journal-title":"Proc. Soc. Exptl. Biol. Med."}],"container-title":["Journal of Bacteriology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jb.83.1.61-65.1962","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jb.83.1.61-65.1962","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T17:07:00Z","timestamp":1627578420000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/jb.83.1.61-65.1962"}},"subtitle":["Nutritional Requirements of\n            <i>Shigella Flexneri<\/i>\n            Serotype"],"short-title":[],"issued":{"date-parts":[[1962,1]]},"references-count":6,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1962,1]]}},"alternative-id":["10.1128\/jb.83.1.61-65.1962"],"URL":"https:\/\/doi.org\/10.1128\/jb.83.1.61-65.1962","relation":{},"ISSN":["0021-9193","1098-5530"],"issn-type":[{"value":"0021-9193","type":"print"},{"value":"1098-5530","type":"electronic"}],"subject":[],"published":{"date-parts":[[1962,1]]},"assertion":[{"value":"2021-02-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}