{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T18:13:30Z","timestamp":1761156810055},"reference-count":18,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[1963,1,1]],"date-time":"1963-01-01T00:00:00Z","timestamp":-220924800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[1963,1]]},"abstract":"<jats:p><jats:sc>Puziss, Milton<\/jats:sc>(Fort Detrick, Frederick, Md.) and<jats:sc>George G. Wright<\/jats:sc>. Studies on immunity in anthrax. X. Gel-adsorbed protective antigen for immunization of man. J. Bacteriol.<jats:bold>85:<\/jats:bold>230\u2013236. 1963.\u2014Protective antigen in filtrates of anaerobic cultures of<jats:italic>Bacillus anthracis<\/jats:italic>was not readily precipitable by alum or other aluminum salts, but was adsorbed onto a preformed aluminum hydroxide gel under specific conditions. The adsorbed product was highly effective in immunizing rabbits, guinea pigs, and mice against challenge with virulent<jats:italic>B. anthracis<\/jats:italic>spores. Gel-adsorbed anaerobic antigens preserved with 1:10,000 thimerosal were unstable on storage at 4 C; replacement of this preservative with 1:40,000 benzethonium chloride produced a more stable product. Addition of 0.0009% formalin further increased the stability during accelerated aging at 37 C. The final product had high protective activity in animals and was well tolerated in man.<\/jats:p>","DOI":"10.1128\/jb.85.1.230-236.1963","type":"journal-article","created":{"date-parts":[[2020,1,3]],"date-time":"2020-01-03T21:24:56Z","timestamp":1578086696000},"page":"230-236","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":62,"title":["STUDIES ON IMMUNITY IN ANTHRAX X"],"prefix":"10.1128","volume":"85","author":[{"given":"Milton","family":"Puziss","sequence":"first","affiliation":[{"name":"U.S. Army Biological Laboratories, Fort Detrick, Frederick, Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"George G.","family":"Wright","sequence":"additional","affiliation":[{"name":"U.S. Army Biological Laboratories, Fort Detrick, Frederick, Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"p_1","doi-asserted-by":"crossref","first-page":"1274","DOI":"10.1128\/JB.83.6.1274-1280.1962","article-title":"Rapid lethal effect in rats of a third component found upon fractionating the toxin of Bacillus anthracis","volume":"83","author":"BEALL F. 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