{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T22:40:55Z","timestamp":1761864055811},"reference-count":8,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[1963,4,1]],"date-time":"1963-04-01T00:00:00Z","timestamp":-213148800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[1963,4]]},"abstract":"\n Di Salvo, Arthur<\/jats:sc>\n F. (Medical College of Georgia, Augusta)\n and J. Fred Denton<\/jats:sc>\n . Lipid content of four strains of\n Blastomyces dermatitidis<\/jats:italic>\n of different mouse virulence. J. Bacteriol.\n 85:<\/jats:bold>\n 927\u2013931. 1963.\u2014Four strains of\n Blastomyces dermatitidis<\/jats:italic>\n showing marked differences in virulence for mice were studied to determine whether there was any relationship between lipid content and virulence. Replicate samples of heat-killed yeast and mycelial cells, after rupturing by grinding, were extracted with a chloroform-methanol mixture (2:1) in a Soxhlet apparatus for 48 hr. Total extractable lipid was computed by evaporating samples of the extracts to dryness and weighing; the phospholipid fraction was determined by a modified photometric technique. The amount of lipid in the yeast phase of each strain was relatively constant and characteristic as determined by extraction of four samples of each. The extractable lipid increased from 6.8% in the avirulent strain Ga-1 to 12.3% in the highly virulent strain SL-1, the lipid content correlating directly with increased mouse virulence. There was no similar correlation of phospholipid content with mouse virulence. The mycelial phases of two strains contained more than double the lipid and phospholipid present in the corresponding yeast phases. Data presented suggest that the lipid fraction of the yeast phase of\n B. dermatitidis<\/jats:italic>\n , in addition to being related to the granulomatous reaction, is possibly related to virulence.\n <\/jats:p>","DOI":"10.1128\/jb.85.4.927-931.1963","type":"journal-article","created":{"date-parts":[[2020,1,3]],"date-time":"2020-01-03T21:25:46Z","timestamp":1578086746000},"page":"927-931","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":50,"title":["LIPID CONTENT OF FOUR STRAINS OF\n BLASTOMYCES DERMATITIDIS<\/i>\n OF DIFFERENT MOUSE VIRULENCE"],"prefix":"10.1128","volume":"85","author":[{"given":"Arthur F.","family":"Di Salvo","sequence":"first","affiliation":[{"name":"Department of Microbiology, Medical College of Georgia, Augusta, Georgia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. Fred","family":"Denton","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Medical College of Georgia, Augusta, Georgia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"p_1","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1128\/JB.80.4.565-566.1960","article-title":"Free lipids and phospholipid phosphorous of Histoplasma capsulatum and other pathogenic fungi","volume":"80","author":"AL-DOORY Y.","year":"1960","unstructured":"AL-DOORY , Y. 1960 . Free lipids and phospholipid phosphorous of Histoplasma capsulatum and other pathogenic fungi . J. Bacteriol. 80 : 565 - 566 .","journal-title":"J. Bacteriol."},{"key":"p_2","first-page":"463","article-title":"Experimental blastomycosis in mice","volume":"18","author":"BAKER R. D.","year":"1942","unstructured":"BAKER , R. D. 1942 . Experimental blastomycosis in mice . Am. J. Pathol. 18 : 463 - 478 .","journal-title":"Am. J. Pathol."},{"key":"p_3","first-page":"34","article-title":"North American blastomycosis","volume":"40","author":"CHICK E. W.","year":"1960","unstructured":"CHICK , E. W. , H. J. PETERS , J. F. DENTON , A ND W . D. BORING. 1960 . North American blastomycosis . Ergeb. Allgem. Pathol. Pathol. Anat. 40 : 34 - 98 .","journal-title":"Ergeb. Allgem. Pathol. Pathol. Anat."},{"key":"p_4","unstructured":"COCHRANE V. W. 1958. Physiology of the fungi. John Wiley & Sons Inc.. New York."},{"key":"p_5","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1097\/00000441-195809000-00005","article-title":"Chemical constituents of pine pollen and their possible relationship to sarcoidosis","volume":"236","author":"CUMMINGS M. M.","year":"1958","unstructured":"CUMMINGS , M. M. , AND P. C. HUDGINS. 1958 . Chemical constituents of pine pollen and their possible relationship to sarcoidosis . Am. J. Med. Sci. 236 : 311 - 317 .","journal-title":"Am. J. Med. Sci."},{"key":"p_6","doi-asserted-by":"crossref","first-page":"1126","DOI":"10.1126\/science.133.3459.1126","article-title":"Isolation of Blastomyces dermatitidis from soil","volume":"133","author":"DENTON J. F.","year":"1961","unstructured":"DENTON , J. F. , E. S. Mc DONOUGH , L. AJELLO , A ND R . J. AUSHERMAN. 1961 . Isolation of Blastomyces dermatitidis from soil . Science 133 : 1126 - 1127 .","journal-title":"Science"},{"key":"p_7","unstructured":"HAWK P. B. B. L. OSER AND W. H. SUMMERSON. 1947. Practical physiological chemistry 12th ed. The Blakiston Co. Philadelphia."},{"key":"p_8","unstructured":"LAMANNA C. AND M. F. MALLETTE. 1954. Use of glass beads for the mechanical rupture of 931"}],"container-title":["Journal of Bacteriology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jb.85.4.927-931.1963","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jb.85.4.927-931.1963","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T17:12:41Z","timestamp":1627578761000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/jb.85.4.927-931.1963"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1963,4]]},"references-count":8,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1963,4]]}},"alternative-id":["10.1128\/jb.85.4.927-931.1963"],"URL":"https:\/\/doi.org\/10.1128\/jb.85.4.927-931.1963","relation":{},"ISSN":["0021-9193","1098-5530"],"issn-type":[{"value":"0021-9193","type":"print"},{"value":"1098-5530","type":"electronic"}],"subject":[],"published":{"date-parts":[[1963,4]]},"assertion":[{"value":"2021-02-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}