{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,25]],"date-time":"2023-09-25T09:53:15Z","timestamp":1695635595661},"reference-count":15,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[1963,6,1]],"date-time":"1963-06-01T00:00:00Z","timestamp":-207878400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[1963,6]]},"abstract":"<jats:p><jats:sc>Riley, Jean<\/jats:sc>M. (U.S. Army Biological Laboratories, Fort Detrick, Frederick, Md.),<jats:sc>William C. Patrick, III, and William E. Campbell, Jr.<\/jats:sc>Estimation of titer of Venezuelan equine encephalomyelitis virus preparations from a single-dilution assay. J. Bacteriol.<jats:bold>85:<\/jats:bold>1256\u20131260. 1963.\u2014When suspensions of Venezuelan equine encephalomyelitis (VEE) virus were injected intracerebrally into groups of mice, a nearly linear relationship was observed between the concentration of the virus injected and the mean reciprocal time-to-death of the mice. A total of 91 VEE preparations were assayed in duplicate, and, by plotting the relationship between the reciprocal time-to-death for mice given the 10<jats:sup>\u22126<\/jats:sup>dilution of virus and the<jats:sc>micld<\/jats:sc><jats:sub>50<\/jats:sub>(mouse intracerebral challenge,<jats:sc>ld<\/jats:sc><jats:sub>50<\/jats:sub>response) values for the virus preparations, a reference curve was established. Using this reference curve, it was possible to estimate directly the<jats:sc>ld<\/jats:sc><jats:sub>50<\/jats:sub>values of virus suspensions of unknown concentration from the mean reciprocal time-to-death of a group of mice injected with a single dilution. In this work, the number of mice used was reduced by 62.5%, the titrations were complete in 3 to 5 days compared with the usual 10 to 14 days, three to four times as many assays could be done in a day, and no assays had to be repeated since end points were not missed. The precision of the single-dilution assay compared favorably with that of the<jats:sc>ld<\/jats:sc><jats:sub>50<\/jats:sub>titration.<\/jats:p>","DOI":"10.1128\/jb.85.6.1256-1260.1963","type":"journal-article","created":{"date-parts":[[2020,1,3]],"date-time":"2020-01-03T21:26:19Z","timestamp":1578086779000},"page":"1256-1260","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":2,"title":["ESTIMATION OF TITER OF VENEZUELAN EQUINE ENCEPHALOMYELITIS VIRUS PREPARATIONS FROM A SINGLE-DILUTION ASSAY"],"prefix":"10.1128","volume":"85","author":[{"given":"Jean M.","family":"Riley","sequence":"first","affiliation":[{"name":"United States Army Biological Laboratories, Fort Detrick, Frederick, Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"III","given":"William C.","family":"Patrick","sequence":"additional","affiliation":[{"name":"United States Army Biological Laboratories, Fort Detrick, Frederick, Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr.","given":"William E.","family":"Campbell","sequence":"additional","affiliation":[{"name":"United States Army Biological Laboratories, Fort Detrick, Frederick, Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"p_1","first-page":"130","article-title":"Some applications of a singledilution method of titrating neurotropic viruses in zero mortality (Do) units","volume":"41","author":"BAUER D. 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