{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T13:38:50Z","timestamp":1761745130579},"reference-count":13,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[1965,7,1]],"date-time":"1965-07-01T00:00:00Z","timestamp":-142128000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Bacteriol"],"published-print":{"date-parts":[[1965,7]]},"abstract":"<jats:p><jats:sc>Formal, Samuel<\/jats:sc>B. (Walter Reed Army Institute of Research, Washington, D.C.), E. H.<jats:sc>LaBrec, Amos Palmer, and Stanley Falkow<\/jats:sc>. Protection of monkeys against experimental shigellosis with attenuated vaccines. J. Bacteriol.<jats:bold>90:<\/jats:bold>63\u201368. 1965.\u2014Two<jats:italic>Shigella flexneri<\/jats:italic>2a strains of reduced virulence were used as oral vaccines to protect monkeys against experimental challenge. One strain, a spontaneous mutant, had lost its ability to cause disease and was unable to penetrate the intestinal epithelium and reach the lamina propria. The other strain was a hybrid obtained by mating virulent<jats:italic>S. flexneri<\/jats:italic>2a with<jats:italic>Escherichia coli<\/jats:italic>. This hybrid strain retained the capacity to penetrate the intestinal epithelium but was not able to maintain itself in the lamina propria. Five oral doses of the nonpenetrating mutant strain were required to render monkeys resistant to experimental challenge, but a single dose of the hybrid strain sufficed to protect the animals. There was some evidence that a degree of specificity was involved in the induced resistance, although neither vaccine evoked a consistent serum antibody or a detectable coproantibody response.<\/jats:p>","DOI":"10.1128\/jb.90.1.63-68.1965","type":"journal-article","created":{"date-parts":[[2020,1,3]],"date-time":"2020-01-03T20:50:36Z","timestamp":1578084636000},"page":"63-68","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":54,"title":["Protection of Monkeys Against Experimental Shigellosis with Attenuated Vaccines"],"prefix":"10.1128","volume":"90","author":[{"given":"Samuel B.","family":"Formal","sequence":"first","affiliation":[{"name":"Walter Reed Army Institute of Research, Washington, D.C."},{"name":"Division of Research Services, National Institutes of Health, Bethesda, Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E. H.","family":"LaBrec","sequence":"additional","affiliation":[{"name":"Walter Reed Army Institute of Research, Washington, D.C."},{"name":"Division of Research Services, National Institutes of Health, Bethesda, Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amos","family":"Palmer","sequence":"additional","affiliation":[{"name":"Walter Reed Army Institute of Research, Washington, D.C."},{"name":"Division of Research Services, National Institutes of Health, Bethesda, Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stanley","family":"Falkow","sequence":"additional","affiliation":[{"name":"Walter Reed Army Institute of Research, Washington, D.C."},{"name":"Division of Research Services, National Institutes of Health, Bethesda, Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"p_1","unstructured":"BATSON H. C. 1956. An introduction to statistics in medical sciences. Burgess Publishing Co. Minneapolis."},{"key":"p_2","unstructured":"CRUICKSHANK R. 1963. Acquired immunity: bacterial infections p. 119. In R. Cruickshank [ed.] Modern trends in immunology. Butterworth Inc. Washington D.C."},{"key":"p_3","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1002\/path.1700740218","article-title":"Fimbriae and adhesive properties in dysentery bacilli","volume":"74","author":"DUGUID J. P.","year":"1957","unstructured":"DUGUID , J. P. , AND R. R. GILLIES. 1957 . Fimbriae and adhesive properties in dysentery bacilli . J. Pathol. Bacteriol. 74 : 397 - 411 .","journal-title":"J. Pathol. Bacteriol."},{"key":"p_4","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1093\/infdis\/103.3.239","article-title":"A quantitative immunochemical measure of the primary interaction between I* BSA and antibody","volume":"103","author":"FARR R. S.","year":"1958","unstructured":"FARR , R. S. 1958 . 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